『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
ID: Pass:

登録内容 (EID=99698)

EID=99698EID:99698, Map:0, LastModified:2016年9月9日(金) 16:21:54, Operator:[三木 ちひろ], Avail:TRUE, Censor:0, Owner:[[学科長]/[徳島大学.歯学部.歯学科]], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨):
カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1.内田 大亮
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
2. (英) Begum Mila Nasima (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
3. (英) Almofti Ammar (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
4. (英) Kawamata Hitoshi (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
5.吉田 秀夫
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
6.佐藤 光信
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
題名 (必須): (英) Frequent Down-regulation of 14-3-3 sigma protein and Hypermethylation of 14-3-3 sigma gene in Salivary Gland Adenoid Cystic Carcinoma  (日)    [継承]
副題 (任意):
要約 (任意): (英) 14-3-3 sigma:, a target gene of the p53 tumour suppressor protein, has been shown to regulate the cell cycle at the G2/M checkpoint. Recent studies have demonstrated that 14-3-3 sigma is downregulated by hypermethylation of the CpG island in several types of cancer. In this study, we investigated the expression and methylation status of 14-3-3 sigma in human salivary gland adenoid cystic carcinoma (ACC) and mucoepidermoid carcinoma (MEC). Immunohistochemical analysis revealed that the positive expression rate of 14-3-3 sigma in ACC (one out of 14) was markedly lower than that in MEC (ten out of 10). Since most of the ACCs carried the wild-type p53 protein, downregulation of 14-3-3 sigma in ACC may not be due to the dysfunction of p53 pathway. Microdissection-methylation-specific PCR revealed that frequent hypermethylation of the 14-3-3 sigma gene was observed in ACC when compared to that in MEC. In cultured-ACC cells, we confirmed the downregulation of 14-3-3 sigma via hemimethylation of the gene by sequencing analysis after sodium bisulphite treatment. Furthermore, re-expression of 14-3-3 sigma in the ACC cells was induced by the treatment with DNA demethylating agent, 5-aza-2'-deoxycytidine. Irradiation apparently induced the enhanced expression of 14-3-3 sigma and G2/M arrest in normal salivary gland cells; however, in the ACC cells, neither induction of 14-3-3 sigma nor G2/M arrest was induced by irradiation. These results suggest that downregulation of 14-3-3 sigma might play critical roles in the neoplastic development and radiosensitivity of ACC.  (日)    [継承]
キーワード (推奨): 1. (英) 14-3-3 Proteins (日) (読) [継承]
2. (英) Base Sequence (日) (読) [継承]
3. (英) Carcinoma, Adenoid Cystic (日) (読) [継承]
4. (英) DNA Methylation (日) (読) [継承]
5. (英) DNA Primers (日) (読) [継承]
6. (英) Exonucleases (日) (読) [継承]
7. (英) Genes, p53 (日) (読) [継承]
8. (英) Humans (日) (読) [継承]
9.免疫組織化学 (immunohistochemistry) [継承]
10. (英) Mutation (日) (読) [継承]
11. (英) Neoplasm Proteins (日) (読) [継承]
12. (英) Polymerase Chain Reaction (日) (読) [継承]
13. (英) Salivary Gland Neoplasms (日) (読) [継承]
14. (英) Tumor Markers, Biological (日) (読) [継承]
発行所 (推奨):
誌名 (必須): British Journal of Cancer (British Empire Cancer Campaign/Cancer Research UK)
(pISSN: 0007-0920, eISSN: 1532-1827)

ISSN (任意): 0007-0920
ISSN: 0007-0920 (pISSN: 0007-0920, eISSN: 1532-1827)
Title: British journal of cancer
Title(ISO): Br. J. Cancer
Publisher: Nature Publishing Group
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
[継承]
[継承]
(必須): 91 [継承]
(必須): 6 [継承]
(必須): 1131 1138 [継承]
都市 (任意):
年月日 (必須): 西暦 2004年 8月 3日 (平成 16年 8月 3日) [継承]
URL (任意):
DOI (任意): 10.1038/sj.bjc.6602004    (→Scopusで検索) [継承]
PMID (任意): 15292943    (→Scopusで検索) [継承]
NAID (任意):
WOS (任意): 000223885200020 [継承]
Scopus (任意): 2-s2.0-5044234175 [継承]
評価値 (任意):
被引用数 (任意):
指導教員 (推奨):
備考 (任意): 1.(英) Article.Affiliation: Second Department of Oral and Maxillofacial Surgery, Tokushima University School of Dentistry, 3-18-15 Kuramoto, Tokushima 770-8504, Japan. daisuke@dent.tokushima-u.ac.jp  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]
4.(英) OtherID: PMC2747720  (日)    [継承]

標準的な表示

和文冊子 ● Daisuke Uchida, Nasima Mila Begum, Ammar Almofti, Hitoshi Kawamata, Hideo Yoshida and Mitsunobu Sato : Frequent Down-regulation of 14-3-3 sigma protein and Hypermethylation of 14-3-3 sigma gene in Salivary Gland Adenoid Cystic Carcinoma, British Journal of Cancer, Vol.91, No.6, 1131-1138, 2004.
欧文冊子 ● Daisuke Uchida, Nasima Mila Begum, Ammar Almofti, Hitoshi Kawamata, Hideo Yoshida and Mitsunobu Sato : Frequent Down-regulation of 14-3-3 sigma protein and Hypermethylation of 14-3-3 sigma gene in Salivary Gland Adenoid Cystic Carcinoma, British Journal of Cancer, Vol.91, No.6, 1131-1138, 2004.

関連情報

Number of session users = 0, LA = 0.54, Max(EID) = 361611, Max(EOID) = 967818.