| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○招待 (推奨): |
| ○審査 (推奨): |
| ○カテゴリ (推奨): |
| ○共著種別 (推奨): |
| ○学究種別 (推奨): |
| ○組織 (推奨): | 1. | 徳島大学.大学院ヘルスバイオサイエンス研究部.神経情報医学部門.情報統合医学講座 (2004年4月1日〜)
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| ○著者 (必須): | 1. | 中村 教泰
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| 2. | (英) Tsumoto Kouhei (日) (読)
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| 3. | 石村 和敬
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| 4. | (英) Kumagai Izumi (日) (読)
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| ○題名 (必須): | □ | (英) Detection of biotinylated proteins in polyacrylamide gels using an avidin-fluorescein conjugate. (日)
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| ○副題 (任意): |
| ○要約 (任意): | □ | (英) Biotinylated proteins are widely used as a molecular tool in biotechnological applications. In this paper, we demonstrated that biotinylated proteins after electrophoresis were detected directly in gels using an avidin-fluorescein conjugate with a fluorescence image analyzer. Upon analysis of the purified and chemically biotinylated protein, the sensitivity of this method was almost equal to that of silver staining. Chemically biotinylated proteins of Escherichia coli cell surfaces could also be specifically detected with our method. Furthermore, recombinant proteins fused with the biotin acceptor domain and biotinylated enzymatically in vivo were also detected in a lysate of E. coli specifically. The sensitivity and specificity of our method are high, and the procedure is simple. Therefore, our method would benefit detection of biotinylated proteins via gel electrophoresis and also various fields of study using avidin-biotin technology. (日)
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| ○キーワード (推奨): | 1. | (英) Avidin (日) (読)
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| 2. | (英) Bacterial Proteins (日) (読)
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| 3. | (英) Biotin (日) (読)
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| 4. | (英) Biotinylation (日) (読)
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| 5. | (英) Carbon-Nitrogen Ligases (日) (読)
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| 6. | (英) Cell Culture Techniques (日) (読)
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| 7. | (英) Centrifugation (日) (読)
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| 8. | (英) Electrophoresis, Polyacrylamide Gel (日) (読)
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| 9. | (英) Escherichia coli (日) (読)
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| 10. | (英) Escherichia coli Proteins (日) (読)
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| 11. | (英) Fluoresceins (日) (読)
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| 12. | (英) Glutathione Transferase (日) (読)
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| 13. | (英) Humans (日) (読)
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| 14. | (英) Immunoblotting (日) (読)
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| 15. | (英) Immunoglobulin Heavy Chains (日) (読)
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| 16. | (英) Immunoglobulin Variable Region (日) (読)
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| 17. | (英) Membrane Proteins (日) (読)
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| 18. | (英) Polymerase Chain Reaction (日) (読)
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| 19. | (英) Proteins (日) (読)
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| 20. | (英) Repressor Proteins (日) (読)
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| 21. | (英) Sensitivity and Specificity (日) (読)
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| 22. | (英) Silver Staining (日) (読)
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| 23. | (英) Transcription Factors (日) (読)
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| ○発行所 (推奨): |
| ○誌名 (必須): | □ | Analytical Biochemistry: Methods in the Biological Sciences ([Elsevier Science])
(pISSN: 0003-2697, eISSN: 1096-0309)
| ○ISSN (任意): | □ | 0003-2697
ISSN: 0003-2697
(pISSN: 0003-2697, eISSN: 1096-0309) Title: Analytical biochemistryTitle(ISO): Anal BiochemPublisher: Elsevier Inc. (NLM Catalog)
(Scopus)
(CrossRef)
(Scopus information is found. [need login]) | [継承] |
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| ○巻 (必須): | □ | 304
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| ○号 (必須): | □ | 2
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| ○頁 (必須): | □ | 231 235
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| ○都市 (任意): |
| ○年月日 (必須): | □ | 西暦 2002年 5月 15日 (平成 14年 5月 15日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1006/abio.2002.5595 (→Scopusで検索)
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| ○PMID (任意): | □ | 12009700 (→Scopusで検索)
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| ○NAID (任意): |
| ○WOS (任意): | □ | 000175640400012
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| ○指導教員 (推奨): |
| ○備考 (任意): | 1. | (英) Article.Affiliation: Department of Anatomy and Cell Biology, School of Medicine, Tokushima University, 3-18-15 Kuramoto, Tokushima 770-8503, Japan. (日)
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| 2. | (英) Article.PublicationTypeList.PublicationType: Comparative Study (日)
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| 3. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
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| 4. | (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't (日)
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