『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
ID: Pass:

登録内容 (EID=93788)

EID=93788EID:93788, Map:0, LastModified:2013年6月17日(月) 16:36:17, Operator:[大家 隆弘], Avail:TRUE, Censor:0, Owner:[[研究部長]/[徳島大学.大学院ヘルスバイオサイエンス研究部]], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨): Peer Review [継承]
カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨): 1.徳島大学.大学院ヘルスバイオサイエンス研究部.発達予防医歯学部門.健康長寿歯科学講座 (2004年4月1日〜) [継承]
2.日本大学.薬学部臨床薬剤学講座 [継承]
著者 (必須): 1. (英) Kugawa Fumihiko (日) 九川 文彦 (読) くがわ ふみひこ
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
2.上野 明道
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
3. (英) Kawasaki Miho (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
4. (英) Aoki Masatada (日) 青木 正忠 (読) あおき まさただ
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
題名 (必須): (英) Evaluation of cell death caused by CDF (cyclophosphamide, doxorubicin, 5-fluorouracil) multi-drug administration in the human breast cancer cell line MCF-7.  (日)    [継承]
副題 (任意):
要約 (任意): (英) We evaluated the features of cell death induced by CDF (cyclophosphamide [CPA], doxorubicin [DOX], 5-fluorouracil [5-FU]) multi-drug administration in vitro using the human breast cancer cell line MCF-7. Used individually, DOX and 5-FU induced 60% cell death in MCF-7 cells, at 5 microg/ml and 25 microg/ml, respectively, by the 4th day following drug treatment. CPA was the least cytotoxic of the 3 drugs, causing only 20% cell death, even at the high concentration of 500 microg/ml. Treating cells with a mixture of all three anticancer drugs resulted in 60% cell death, on the second and third day following drug treatment. The nature of the cytotoxicity of CPA, DOX, and 5-FU was investigated, because these drugs are sometimes used to induce apoptosis. Biochemical analysis showed faint DNA fragmentation in the case of DOX or all three drugs, but not for treatment with CPA or 5-FU. In contrast, the morphological apoptotic feature of a condensed nucleus was observed only for CPA and 5-FU. Flow cytometric data agreed with the morphological results in that the FACS cytogram for DOX and for all three drugs was different from that for CPA or 5-FU given alone. These observations suggested that the cell death induced by these anticancer drugs in the human breast cancer cell line MCF-7 is a mixture of apoptotic and non-apoptotic, but it becomes completely non-apoptotic in the case of multi-drug administration.  (日)    [継承]
キーワード (推奨): 1. (英) Breast Neoplasms (日) (読) [継承]
2. (英) Cell Death (日) (読) [継承]
3. (英) Cell Line, Tumor (日) (読) [継承]
4. (英) Cell Survival (日) (読) [継承]
5. (英) Cyclophosphamide (日) (読) [継承]
6. (英) Doxorubicin (日) (読) [継承]
7. (英) Drug Synergism (日) (読) [継承]
8. (英) Flow Cytometry (日) (読) [継承]
9. (英) Fluorouracil (日) (読) [継承]
10. (英) Humans (日) (読) [継承]
11. (英) Time Factors (日) (読) [継承]
発行所 (推奨): 日本薬学会 [継承]
誌名 (必須): Biological & Pharmaceutical Bulletin ([日本薬学会])
(pISSN: 0918-6158, eISSN: 1347-5215)

ISSN (任意): 0918-6158
ISSN: 0918-6158 (pISSN: 0918-6158, eISSN: 1347-5215)
Title: Biological & pharmaceutical bulletin
Title(ISO): Biol Pharm Bull
Supplier: 公益社団法人日本薬学会
Publisher: Pharmaceutical Society of Japan
 (NLM Catalog  (Webcat Plus  (医中誌Web  (J-STAGE  (Scopus  (CrossRef (Scopus information is found. [need login])
[継承]
[継承]
(必須): 27 [継承]
(必須): 3 [継承]
(必須): 392 398 [継承]
都市 (任意): 東京 (Tokyo/[日本国]) [継承]
年月日 (必須): 西暦 2004年 3月 1日 (平成 16年 3月 1日) [継承]
URL (任意):
DOI (任意): 10.1248/bpb.27.392    (→Scopusで検索) [継承]
PMID (任意): 14993808    (→Scopusで検索) [継承]
NAID (任意):
WOS (任意):
Scopus (任意):
評価値 (任意):
被引用数 (任意):
指導教員 (推奨):
備考 (任意): 1.(英) Article.Affiliation: Department of Biological Pharmaceutical Sciences, College of Pharmacy, Nihon-University, Narashino-dai, Funabashi, Chiba, Japan. jfkugawa@pha.nihon-u.ac.jp  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Fumihiko Kugawa, Akemichi Ueno, Miho Kawasaki and Masatada Aoki : Evaluation of cell death caused by CDF (cyclophosphamide, doxorubicin, 5-fluorouracil) multi-drug administration in the human breast cancer cell line MCF-7., Biological & Pharmaceutical Bulletin, Vol.27, No.3, 392-398, 2004.
欧文冊子 ● Fumihiko Kugawa, Akemichi Ueno, Miho Kawasaki and Masatada Aoki : Evaluation of cell death caused by CDF (cyclophosphamide, doxorubicin, 5-fluorouracil) multi-drug administration in the human breast cancer cell line MCF-7., Biological & Pharmaceutical Bulletin, Vol.27, No.3, 392-398, 2004.

関連情報

Number of session users = 3, LA = 0.69, Max(EID) = 382710, Max(EOID) = 1022120.