『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=88541EID:88541, Map:0, LastModified:2013年7月9日(火) 11:38:02, Operator:[三木 ちひろ], Avail:TRUE, Censor:0, Owner:[上原 久典], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨):
カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨): 1.徳島大学.大学院ヘルスバイオサイエンス研究部.再生修復医歯学部門.生体防御腫瘍医学講座 (2004年4月1日〜2015年3月31日) [継承]
著者 (必須): 1.上原 久典 ([徳島大学.病院.中央診療施設等.病理部])
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
2. (英) Kim Sun-Jin (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
3. (英) Karashima Takashi (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
4. (英) Shepherd L. David (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
5. (英) Fan Dominic (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
6. (英) Tsan Rachel (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
7. (英) Killion J. Jerald (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
8. (英) Logothetis Christopher (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
9. (英) Mathew Paul (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
10. (英) Fidler J Isaiah (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
題名 (必須): (英) Effects of blocking platelet-derived growth factor-receptor signaling in a mouse model of experimental prostate cancer bone metastases  (日)    [継承]
副題 (任意):
要約 (任意): (英) Expression of platelet-derived growth factor (PDGF) and activation (by autophosphorylation) of its receptor (PDGF-R), a tyrosine kinase, are associated with the growth of metastatic prostate tumor cells in the bone parenchyma. The tyrosine kinase inhibitor STI571 blocks the PDGF signaling pathway by inhibiting PDGF-R autophosphorylation. We examined the effects of STI571, given alone or with paclitaxel (Taxol), on tumor growth in a mouse model of prostate cancer metastasis. Human prostate cancer PC-3MM2 cells were injected into the tibias of male nude mice. Three days later the mice (20 per group) were randomly assigned to 5 weeks of treatment with oral and injected water (control), daily oral STI571, weekly injected paclitaxel, or STI571 plus paclitaxel. Lesions in bone and the surrounding muscles were then harvested and analyzed by histology, western blotting (for PDGF-R phosphorylation), immunohistochemistry (for expression of proangiogenic molecules), and double immunofluorescence (to identify endothelial cells and apoptotic tumor cells). Growth of bone lesions was monitored by digital radiography. Bone lesions from control mice were used to establish short-term cell cultures for analysis of PDGF-R phosphorylation. All statistical tests were two-sided. PC-3MM2 cells cultured from bone lesions and treated in vitro with STI571 had less phosphorylated PDGF-R than untreated cells. In control mice, bone lesions expressed high levels of PDGF and activated (i.e., phosphorylated) PDGF-R, whereas lesions in the adjacent musculature did not. Activated PDGF-R was present on the surface of endothelial cells within the bone lesions but not in endothelial cells of uninjected bone. Mice treated with STI571 or STI571 plus paclitaxel had a lower tumor incidence, smaller tumors, and less bone lysis and lymph node metastasis than mice treated with water or paclitaxel alone (P<.001 for all). Mice treated with STI571 or STI571 plus paclitaxel had less phosphorylated PDGF-R on tumor cells and tumor-associated endothelial cells, less tumor cell proliferation, statistically significantly more apoptotic tumor cells (all P<.001), and fewer tumor-associated endothelial cells (P<.001) than control mice. Endothelial cells appear to express phosphorylated PDGF-R when they are exposed to tumor cells that express PDGF. Using STI571 to inhibit PDGF-R phosphorylation may, especially in combination with paclitaxel, produce substantial therapeutic effects against prostate cancer bone metastasis.  (日)    [継承]
キーワード (推奨): 1. (英) Administration, Oral (日) (読) [継承]
2. (英) Animals (日) (読) [継承]
3. (英) Antineoplastic Agents (日) (読) [継承]
4. (英) Antineoplastic Agents, Phytogenic (日) (読) [継承]
5. (英) Antineoplastic Combined Chemotherapy Protocols (日) (読) [継承]
6.アポトーシス (apoptosis) [継承]
7. (英) Blotting, Western (日) (読) [継承]
8. (英) Bone Neoplasms (日) (読) [継承]
9.細胞分裂 (cell division) [継承]
10. (英) Disease Models, Animal (日) (読) [継承]
11. (英) Enzyme Inhibitors (日) (読) [継承]
12. (英) Fluorescent Antibody Technique (日) (読) [継承]
13. (英) Gene Expression Regulation, Neoplastic (日) (読) [継承]
14. (英) Humans (日) (読) [継承]
15.免疫組織化学 (immunohistochemistry) [継承]
16. (英) In Situ Nick-End Labeling (日) (読) [継承]
17. (英) Male (日) (読) [継承]
18. (英) Mice (日) (読) [継承]
19. (英) Mice, Nude (日) (読) [継承]
20. (英) Microcirculation (日) (読) [継承]
21. (英) Neoplasms, Experimental (日) (読) [継承]
22. (英) Paclitaxel (日) (読) [継承]
23.リン酸化 (phosphorylation) [継承]
24. (英) Piperazines (日) (読) [継承]
25. (英) Platelet-Derived Growth Factor (日) (読) [継承]
26. (英) Prostatic Neoplasms (日) (読) [継承]
27. (英) Protein-Tyrosine Kinases (日) (読) [継承]
28. (英) Pyrimidines (日) (読) [継承]
29. (英) Radiographic Image Enhancement (日) (読) [継承]
30. (英) Receptors, Platelet-Derived Growth Factor (日) (読) [継承]
31.シグナル伝達 (signal transduction) [継承]
32. (英) Tumor Cells, Cultured (日) (読) [継承]
発行所 (推奨):
誌名 (必須): Journal of the National Cancer Institute (National Cancer Institute (U.S.))
(pISSN: 0027-8874, eISSN: 1460-2105)

ISSN (任意): 0027-8874
ISSN: 0027-8874 (pISSN: 0027-8874, eISSN: 1460-2105)
Title: Journal of the National Cancer Institute
Title(ISO): J Natl Cancer Inst
Supplier: Oxford University Press
Publisher: Oxford University Press
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 95 [継承]
(必須): 6 [継承]
(必須): 458 470 [継承]
都市 (任意):
年月日 (必須): 西暦 2003年 3月 9日 (平成 15年 3月 9日) [継承]
URL (任意): http://jncicancerspectrum.oxfordjournals.org/cgi/reprint/jnci;95/6/458.pdf [継承]
DOI (任意): 10.1093/jnci/95.6.458    (→Scopusで検索) [継承]
PMID (任意): 12644539    (→Scopusで検索) [継承]
NAID (任意):
WOS (任意): 000181559700010 [継承]
Scopus (任意):
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指導教員 (推奨):
備考 (任意): 1.(英) Article.Affiliation: Department of Cancer Biology, The University of Texas M. D. Anderson Cancer Center, Houston 77030, USA.  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]
4.(英) Article.PublicationTypeList.PublicationType: Research Support, U.S. Gov't, P.H.S.  (日)    [継承]

標準的な表示

和文冊子 ● Hisanori Uehara, Sun-Jin Kim, Takashi Karashima, David L. Shepherd, Dominic Fan, Rachel Tsan, Jerald J. Killion, Christopher Logothetis, Paul Mathew and Isaiah J Fidler : Effects of blocking platelet-derived growth factor-receptor signaling in a mouse model of experimental prostate cancer bone metastases, Journal of the National Cancer Institute, Vol.95, No.6, 458-470, 2003.
欧文冊子 ● Hisanori Uehara, Sun-Jin Kim, Takashi Karashima, David L. Shepherd, Dominic Fan, Rachel Tsan, Jerald J. Killion, Christopher Logothetis, Paul Mathew and Isaiah J Fidler : Effects of blocking platelet-derived growth factor-receptor signaling in a mouse model of experimental prostate cancer bone metastases, Journal of the National Cancer Institute, Vol.95, No.6, 458-470, 2003.

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