『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨): Peer Review [継承]
カテゴリ (推奨): 研究 [継承]
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨): 1.徳島大学.大学院ヘルスバイオサイエンス研究部.生体システム栄養科学部門.摂食機能制御学講座 (2004年4月1日〜2015年3月31日) [継承]
2.徳島大学.歯学部.口腔保健学科.口腔保健基礎学講座 (2007年4月1日〜) [継承]
著者 (必須): 1.岡村 裕彦
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2.森本 景之
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[継承]
3. (英) Fujita Michi (日) (読)
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[継承]
4. (英) Nasu Fumio (日) (読)
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[継承]
5. (英) Sasaki Eiko (日) (読)
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[継承]
6.羽地 達次
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[継承]
題名 (必須): (英) Suppression of Egr-1 expression in human oral squamous carcinoma cells by okadaic acid.  (日)    [継承]
副題 (任意):
要約 (任意): (英) We examined the expression of early growth response-1 (Egr-1) gene in human oral squamous carcinoma cell lines SCCKN and SCC-25 cells and human osteoblastic cell lines Saos-2 and MG63 cells treated with okadaic acid, a potent inhibitor of protein phosphatases type 1 and type 2A. Western blot analysis revealed that Egr-1 was strongly expressed in SCCKN cells and that okadaic acid decreased the expression of Egr-1 protein in these cells. However, Egr-1 was expressed at lower levels in SCC-25, Saos-2, and MG63 cells and transiently increased with the okadaic acid treatment. Suppression of Egr-1 protein expression in okadaic acid-treated SCCKN cells stemmed from the suppression of the Egr-1 mRNA level, as determined by the RT-RCR method. Formaldehyde-fixed and alcohol-permeabilized cultured SCCKN cells were reacted with the anti-Egr-1 antibody using immunohistochemical methods. Intense fluorescence was observed in the nuclei of the control SCCKN cells interacted with anti-Egr-1 antibody. However, only a weak reaction was observed in the nuclei in SCCKN cells treated with okadaic acid. A gel mobility shift assay showed that treatment of SCCKN cells with okadaic acid suppressed Egr-1 binding to the DIG-labeled Egr-1 consensus oligonucleotide probe. The present results indicate that the alteration of phosphorylation states in SCCKN cells regulates Egr-1 binding to its consensus sequence and its expression at the transcriptional level.  (日)    [継承]
キーワード (推奨): 1. (英) Carcinogens (日) (読) [継承]
2. (英) Carcinoma, Squamous Cell (日) (読) [継承]
3. (英) DNA-Binding Proteins (日) (読) [継承]
4. (英) Down-Regulation (日) (読) [継承]
5. (英) Early Growth Response Protein 1 (日) (読) [継承]
6. (英) Electrophoresis, Agar Gel (日) (読) [継承]
7. (英) Gene Expression Regulation (日) (読) [継承]
8. (英) Humans (日) (読) [継承]
9. (英) Immediate-Early Proteins (日) (読) [継承]
10.免疫組織化学 (immunohistochemistry) [継承]
11. (英) Mouth Neoplasms (日) (読) [継承]
12. (英) Neoplasm Proteins (日) (読) [継承]
13. (英) Okadaic Acid (日) (読) [継承]
14. (英) Reverse Transcriptase Polymerase Chain Reaction (日) (読) [継承]
15. (英) Suppression, Genetic (日) (読) [継承]
16. (英) Transcription Factors (日) (読) [継承]
17. (英) Tumor Cells, Cultured (日) (読) [継承]
発行所 (推奨):
誌名 (必須): Oral Oncology (European Association of Oral Medicine/International Association of Oral Pathologists/International Academy of Oral Oncology)
(pISSN: 1368-8375, eISSN: 1879-0593)

ISSN (任意): 1368-8375
ISSN: 1368-8375 (pISSN: 1368-8375, eISSN: 1879-0593)
Title: Oral oncology
Title(ISO): Oral Oncol
Publisher: Elsevier Ltd.
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 779 784 [継承]
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年月日 (必須): 西暦 2002年 12月 初日 (平成 14年 12月 初日) [継承]
URL (任意):
DOI (任意): 10.1016/S1368-8375(02)00039-8    (→Scopusで検索) [継承]
PMID (任意): 12570057    (→Scopusで検索) [継承]
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Scopus (任意): 2-s2.0-0037004193 [継承]
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備考 (任意): 1.(英) Article.Affiliation: Department of Histology and Oral Histology, School of Dentistry, The University of Tokushima, 3-18-15, Kuramoto, Tokushima 770-8504, Japan.  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Hirohiko Okamura, Hiroyuki Morimoto, Michi Fujita, Fumio Nasu, Eiko Sasaki and Tatsuji Haneji : Suppression of Egr-1 expression in human oral squamous carcinoma cells by okadaic acid., Oral Oncology, Vol.38, No.8, 779-784, 2002.
欧文冊子 ● Hirohiko Okamura, Hiroyuki Morimoto, Michi Fujita, Fumio Nasu, Eiko Sasaki and Tatsuji Haneji : Suppression of Egr-1 expression in human oral squamous carcinoma cells by okadaic acid., Oral Oncology, Vol.38, No.8, 779-784, 2002.

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