| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○招待 (推奨): |
| ○審査 (推奨): | □ | Peer Review
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| ○カテゴリ (推奨): | □ | 研究
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| ○共著種別 (推奨): | □ | 国内共著 (徳島大学内研究者と国内(学外)研究者との共同研究 (国外研究者を含まない))
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| ○学究種別 (推奨): |
| ○組織 (推奨): | 1. | 徳島大学.フォトニクス健康フロンティア研究院 (2025年5月1日〜)
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| ○著者 (必須): | 1. | 小野 公嗣
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| 2. | 宍戸 裕二 ([徳島大学.フォトニクス健康フロンティア研究院])
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| 3. | (英) Yamagishi Toshiyuki (日) (読)
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| 4. | 福井 清 ([徳島大学.事務局.研究・産学連携部])
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| ○題名 (必須): | □ | (英) Variability in intracellular localization of D-amino acid oxidase in choroid plexus epithelial cells. (日)
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| ○要約 (任意): | □ | (英) D-amino acid oxidase (DAO; also abbreviated as DAAO) in the brain is primarily expressed in glial cells of the cerebellum and brainstem. In glial cells, DAO metabolizes D-serine, which acts as a co-agonist and regulator of glutamate receptors. We previously reported that DAO is also expressed in epithelial cells of the choroid plexus (CP); however, the subcellular localization and functional significance of DAO in the CP remain unclear. In this study, we performed histological analyses to investigate the subcellular localization of DAO in the CP. We first confirmed DAO expression in the mouse CP. Immunostaining revealed vesicle-like DAO signals in the cell bodies of choroid plexus epithelial cells (CPECs). Super-resolution microscopy was then used to evaluate colocalization with intracellular organelle markers. DAO signals partially colocalized with ATP-binding cassette subfamily D member 3 (PMP70) and peroxisomal targeting signal 1 receptor (PEX5). In contrast, they colocalized with Golgin subfamily A member 2 (GM130), trans-Golgi network integral membrane protein 2 (TGN46), adaptor protein complex AP-2 (AP-2), early endosome antigen 1 (EEA1), Ras-related proteins Rab5a, Rab11, CD63 antigen (CD63), tumor susceptibility gene 101 protein (TSG101), lysosome-associated membrane glycoprotein 1 (LAMP-1), LAMP-2, beclin-1 and microtubule-associated protein 1 light chain 3 (LC3). These findings suggest that, in the CP, DAO is transported from the Golgi apparatus to endosomes and subsequently distributed to multiple vesicular compartments. The presence of DAO in peroxisomes, autophagosomes, lysosomes, and exosomes indicated diverse intracellular localization within CPECs. This distribution may enable efficient metabolism of blood-derived D-serine in CPECs. (日)
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| ○キーワード (推奨): | 1. | (英) autophagosome (日) (読)
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| 2. | (英) choroid plexus epithelial cells (日) (読)
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| 3. | D-アミノ酸酸化酵素 (D-amino acid oxidase)
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| 4. | (英) endosome lysosome peroxisome (日) (読)
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| 5. | (英) trans-Golgi network (日) (読)
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| ○発行所 (推奨): | □ | (英) FEBS press (日) (読)
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| ○誌名 (必須): | □ | The FEBS Journal ([Federation of European Biochemical Societies])
(pISSN: 1742-464X, eISSN: 1742-4658)
| ○ISSN (任意): | □ | 1742-4658
ISSN: 1742-464X
(pISSN: 1742-464X, eISSN: 1742-4658) Title: The FEBS journalTitle(ISO): FEBS JSupplier: Federation of European Biochemical SocietiesPublisher: Wiley Publishing (NLM Catalog)
(Wiley)
(Scopus)
(CrossRef)
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| ○年月日 (必須): | □ | 西暦 2026年 2月 17日 (令和 8年 2月 17日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1111/febs.70459 (→Scopusで検索)
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| ○PMID (任意): | □ | 41700757 (→Scopusで検索)
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| ○備考 (任意): | 1. | (英) PublicationType: Journal Article (日)
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