| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○招待 (推奨): |
| ○審査 (推奨): |
| ○カテゴリ (推奨): |
| ○共著種別 (推奨): | □ | 国内共著 (徳島大学内研究者と国内(学外)研究者との共同研究 (国外研究者を含まない))
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| ○学究種別 (推奨): |
| ○組織 (推奨): |
| ○著者 (必須): | 1. | 榊原 正太郎
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| 2. | 坂根 亜由子 ([徳島大学.フォトニクス健康フロンティア研究院])
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| 3. | 佐々木 卓也 ([徳島大学.事務局.研究・産学連携部])
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| 4. | (英) Shinohara Masakazu (日) (読)
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| 5. | (英) Maruo Tomohiko (日) (読)
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| 6. | 宮田 宗明 ([徳島大学.先端酵素学研究所.重点研究部門])
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| 7. | 水谷 清人 ([徳島大学.先端酵素学研究所.重点研究部門])
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| 8. | (英) Takai Yoshimi (日) (読)
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| ○題名 (必須): | □ | (英) Identification of lysophosphatidic acid in serum as a factor that promotes epithelial apical junctional complex organization (日)
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| ○副題 (任意): |
| ○要約 (任意): | □ | (英) The apical junctional complex (AJC) consists of adherens junctions (AJs) and tight junctions and regulates epithelial integrity and remodeling. However, it is unclear how AJC organization is regulated based on environmental cues. We found here using cultured EpH4 mouse mammary epithelial cells that fetal bovine serum (FBS) in a culture medium showed an activity to promote AJC organization and that FBS showed an activity to promote tight junction formation even in the absence of AJ proteins, such as E-cadherin, αE-catenin, and afadin. Furthermore, we purified the individual factor responsible for these functions from FBS and identified this molecule as lysophosphatidic acid (LPA). In validation experiments, purified LPA elicited the same activity as FBS. In addition, we found that the AJC organization-promoting activity of LPA was mediated through the LPA receptor 1/5 via diacylglycerol-novel PKC and Rho-ROCK pathway activation in a mutually independent, but complementary, manner. We demonstrated that the Rho-ROCK pathway activation-mediated AJC organization was independent of myosin II-induced actomyosin contraction, although this signaling pathway was previously shown to induce myosin II activation. These findings are in contrast to the literature, as previous results suggested an AJC organization-disrupting activity of LPA. The present results indicate that LPA in serum has an AJC organization-promoting activity in a manner dependent on or independent of AJ proteins. (日)
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| ○キーワード (推奨): | 1. | (英) adherens junctions (日) (読)
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| 2. | (英) afadin (日) (読)
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| 3. | (英) apical junctional complex (日) (読)
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| 4. | (英) cadherin (日) (読)
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| 5. | (英) catenin (日) (読)
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| 6. | (英) LPA (日) (読)
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| 7. | (英) tight junctions (日) (読)
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| ○発行所 (推奨): |
| ○誌名 (必須): | □ | The Journal of Biological Chemistry ([The American Society for Biochemistry and Molecular Biology])
(pISSN: 0021-9258, eISSN: 1083-351X)
| ○ISSN (任意): | □ | 0021-9258
ISSN: 0021-9258
(pISSN: 0021-9258, eISSN: 1083-351X) Title: The Journal of biological chemistryTitle(ISO): J Biol ChemPublisher: American Society for Biochemistry and Molecular Biology (NLM Catalog)
(Scopus)
(CrossRef)
(Scopus information is found. [need login]) | [継承] |
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| ○巻 (必須): | □ | 298
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| ○号 (必須): | □ | 10
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| ○頁 (必須): | □ | 102426 102426
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| ○都市 (任意): |
| ○年月日 (必須): | □ | 西暦 2022年 8月 27日 (令和 4年 8月 27日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1016/j.jbc.2022.102426 (→Scopusで検索)
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| ○PMID (任意): | □ | 36030821 (→Scopusで検索)
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| ○CRID (任意): |
| ○Scopus (任意): | 1. | 2-s2.0-85138462310
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| ○備考 (任意): | 1. | (英) Derived from Scopus.AUID(7202243549) (日)
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| 2. | (英) Modified using PubMed.PMID(36030821) (日)
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