| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○組織 (推奨): |
| ○著者 (必須): | 1. | (英) Akieda Kiri (日) 秋枝 紀凜 (読) あきえだ きり
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| ○学籍番号 (推奨): | □ | ****
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| 2. | (英) Takegawa Kazuto (日) 武川 和人 (読) たけがわ かずと
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| ○学籍番号 (推奨): | □ | ****
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| 3. | (英) Ito Takeshi (日) (読)
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| 4. | (英) Nagayama Gaku (日) 長山 岳 (読) ながやま がく
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| ○学籍番号 (推奨): | □ | ****
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| 5. | 山﨑 尚志 ([徳島大学.大学院医歯薬学研究部.薬学域.薬科学部門.創薬科学系.衛生薬学]/[徳島大学.薬学部.創製薬科学科.創薬学講座.衛生薬学]/[徳島大学.薬学研究科.創薬科学専攻])
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| 6. | 長﨑 裕加 (徳島大学医歯薬学研究部・医薬品情報学分野)
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| 7. | (英) Nishino Kohei (日) (読)
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| 8. | 小迫 英尊 ([徳島大学.先端酵素学研究所.基幹研究部門])
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| 9. | 篠原 康雄 ([徳島大学.先端酵素学研究所.基幹研究部門])
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| ○題名 (必須): | □ | (英) Unique Behavior of Bacterially Expressed Rat Carnitine Palmitoyltransferase 2 and Its Catalytic Activity (日)
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| ○要約 (任意): | □ | (英) Mammalian type 2 carnitine parmitoyltransferase (EC 2.3.1.21), abbreviated as CPT2, is an enzyme involved in the translocation of fatty acid into the mitochondrial matrix space, and catalyzes the reaction acylcarnitine + CoA = acyl-CoA + carnitine. When rat CPT2 was expressed in Escherichia coli, its behavior was dependent on the presence or absence of i) its mitochondrial localization sequence and ii) a short amino acid sequence thought to anchor it to the mitochondrial inner membrane: CPT2 containing both sequences behaved as a hydrophobic protein, while recombinant CPT2 lacking both regions behaved as a water soluble protein; if only one region was present, the resultant proteins were observed in both fractions. Because relatively few protein species could be obtained from bacterial lysates as insoluble pellets under the experimental conditions used, selective enrichment of recombinant CPT2 protein containing both hydrophobic sequences was easily achieved. Furthermore, when CPT2 enriched in insoluble fraction was resuspended in an appropriate medium, it showed catalytic activity typical of CPT2: it was completely suppressed by the CPT2 inhibitor, ST1326, but not by the CPT1 inhibitor, malonyl-CoA. Therefore, we conclude that the bacterial expression system is an effective tool for characterization studies of mammalian CPT2. (日)
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| ○キーワード (推奨): | 1. | (英) Animals (日) (読)
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| ○発行所 (推奨): |
| ○誌名 (必須): | □ | Biological & Pharmaceutical Bulletin ([日本薬学会])
(pISSN: 0918-6158, eISSN: 1347-5215)
| ○ISSN (任意): | □ | 1347-5215
ISSN: 0918-6158
(pISSN: 0918-6158, eISSN: 1347-5215) Title: Biological & pharmaceutical bulletinTitle(ISO): Biol Pharm BullSupplier: 公益社団法人日本薬学会Publisher: Pharmaceutical Society of Japan (NLM Catalog)
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| ○巻 (必須): | □ | 47
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| ○号 (必須): | □ | 1
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| ○頁 (必須): | □ | 23 27
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| ○都市 (任意): |
| ○年月日 (必須): | □ | 西暦 2024年 1月 1日 (令和 6年 1月 1日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1248/bpb.b23-00612 (→Scopusで検索)
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| ○PMID (任意): | □ | 38171776 (→Scopusで検索)
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| ○備考 (任意): | 1. | (英) Article.ELocationID: 10.1248/bpb.b23-00612 (日)
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| 2. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
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| 3. | (英) KeywordList.Keyword: ST1326 (日)
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| 4. | (英) KeywordList.Keyword: bacterial expression (日)
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| 5. | (英) KeywordList.Keyword: carnitine palmitoyltransferase (日)
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| 6. | (英) KeywordList.Keyword: mitochondria (日)
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| 7. | (英) KeywordList.Keyword: transport (日)
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