| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○招待 (推奨): |
| ○審査 (推奨): | □ | Peer Review
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| ○カテゴリ (推奨): | □ | 研究
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| ○共著種別 (推奨): | □ | 国際共著 (徳島大学内研究者と国外研究機関所属研究者との共同研究)
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| ○学究種別 (推奨): |
| ○組織 (推奨): |
| ○著者 (必須): | 1. | 増田 真志 ([徳島大学.大学院医歯薬学研究部.医学域.栄養科学部門.医科栄養学系.臨床食管理学])
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| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
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| 2. | (英) Yoshida-Shimizu Risa (日) (読)
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| 3. | (英) Mori Yuki (日) 森 優樹 (読) もり ゆうき
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| ○貢献度 (任意): |
| ○学籍番号 (推奨): | □ | ****
| [ユーザ] |
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| 4. | 大西 康太
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| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| 5. | 足立 雄一郎 ([徳島大学.大学院医歯薬学研究部.医学域.栄養科学部門.医科栄養学系.臨床食管理学])
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| ○貢献度 (任意): |
| ○学籍番号 (推奨): | □ | ****
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| 6. | (英) Sakai Maiko (日) 坂井 麻衣子 (読) さかい まいこ
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| ○貢献度 (任意): |
| ○学籍番号 (推奨): | □ | ****
| [ユーザ] |
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| 7. | (英) Kabutoya Serina (日) 甲谷 芹奈 (読) かぶとや せりな
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): | □ | ****
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| [継承] |
| 8. | 大南 博和
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| 9. | 大南 博和
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| 10. | 山本 浩範
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| 11. | (英) Miyazaki Makoto (日) (読)
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| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| 12. | 竹谷 豊 ([徳島大学.大学院医歯薬学研究部.医学域.栄養科学部門.医科栄養学系.臨床食管理学])
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| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| ○題名 (必須): | □ | (英) Sulforaphane induces lipophagy through the activation of AMPK-mTOR-ULK1 pathway signaling in adipocytes. (日)
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| ○副題 (任意): |
| ○要約 (任意): | □ | (英) Lipophagy, a form of selective autophagy, degrades lipid droplet (LD) in adipose tissue and the liver. The chemotherapeutic isothiocyanate sulforaphane (SFN) contributes to lipolysis through the activation of hormone-sensitive lipase and the browning of white adipocytes. However, the details concerning the regulation of lipolysis in adipocytes by SFN-mediated autophagy remain unclear. In this study, we investigated the effects of SFN on autophagy in the epididymal fat of mice fed a high-fat diet (HFD) or control-fat diet and on the molecular mechanisms of autophagy in differentiated 3T3-L1 cells. Western blotting revealed that the protein expression of lipidated LC3 (LC3-II), an autophagic substrate, was induced after 3T3-L1 adipocytes treatment with SFN. In addition, SFN increased the LC3-II protein expression in the epididymal fat of mice fed an HFD. Immunofluorescence showed that the SFN-induced LC3 expression was co-localized with LDs in 3T3-L1 adipocytes and with perilipin, the most abundant adipocyte-specific protein, in adipocytes of mice fed an HFD. Next, we confirmed that SFN activates autophagy flux in differentiated 3T3-L1 cells using the mCherry-EGFP-LC3 and GFP-LC3-RFP-LC3 probe. Furthermore, we examined the induction mechanisms of autophagy by SFN in 3T3-L1 adipocytes using western blotting. ATG5 knockdown partially blocked the SFN-induced release of fatty acids from LDs in mature 3T3-L1 adipocytes. SFN time-dependently elicited the phosphorylation of AMPK, the dephosphorylation of mTOR, and the phosphorylation of ULK1 in differentiated 3T3-L1 cells. Taken together, these results suggest that SFN may provoke lipophagy through AMPK-mTOR-ULK1 pathway signaling, resulting in partial lipolysis of adipocytes. (日)
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| ○キーワード (推奨): | 1. | (英) 3T3-L1 Cells (日) (読)
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| 2. | (英) AMP-Activated Protein Kinases (日) (読)
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| 3. | (英) Adipocytes, White (日) (読)
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| 4. | (英) Animals (日) (読)
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| 5. | オートファジー (autophagy)
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| 6. | (英) Autophagy-Related Protein-1 Homolog (日) (読)
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| 7. | (英) Isothiocyanates (日) (読)
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| 8. | (英) Lipolysis (日) (読)
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| 9. | (英) Mice (日) (読)
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| 10. | シグナル伝達 (signal transduction)
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| 11. | (英) Sulfoxides (日) (読)
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| 12. | (英) TOR Serine-Threonine Kinases (日) (読)
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| ○発行所 (推奨): |
| ○誌名 (必須): | □ | The Journal of Nutritional Biochemistry ([Elsevier])
(pISSN: 0955-2863, eISSN: 1873-4847)
| ○ISSN (任意): | □ | 1873-4847
ISSN: 0955-2863
(pISSN: 0955-2863, eISSN: 1873-4847) Title: The Journal of nutritional biochemistryTitle(ISO): J Nutr BiochemPublisher: Elsevier Inc. (NLM Catalog)
(Scopus)
(CrossRef)
(Scopus information is found. [need login]) | [継承] |
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| ○巻 (必須): | □ | 106
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| ○号 (必須): |
| ○頁 (必須): | □ | 109017 109017
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| ○都市 (任意): |
| ○年月日 (必須): | □ | 西暦 2022年 4月 21日 (令和 4年 4月 21日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1016/j.jnutbio.2022.109017 (→Scopusで検索)
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| ○PMID (任意): | □ | 35461903 (→Scopusで検索)
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| ○researchmap (任意): |
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| ○指導教員 (推奨): |
| ○備考 (任意): | 1. | (英) Article.ELocationID: S0955-2863(22)00088-2 (日)
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| 2. | (英) Article.ELocationID: 10.1016/j.jnutbio.2022.109017 (日)
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| 3. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
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| 4. | (英) KeywordList.Keyword: Adipose (日)
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| 5. | (英) KeywordList.Keyword: Autophagy (日)
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| 6. | (英) KeywordList.Keyword: Cell biology (日)
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| 7. | (英) KeywordList.Keyword: Dietary factors (日)
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| 8. | (英) KeywordList.Keyword: Lipid droplets (日)
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| 9. | (英) KeywordList.Keyword: Obesity (日)
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