| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○著者 (必須): | 1. | (英) Jamal Mostofa (日) (読)
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| 2. | 伊藤 明日香 ([徳島大学.大学院医歯薬学研究部.医学域.先端医学教育研究プロジェクト]/[徳島大学.大学院医歯薬学研究部.医学域.医科学部門.社会医学系.法医学])
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| 3. | (英) Miki Takanori (日) (読)
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| 4. | (英) Suzuki Shingo (日) (読)
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| 5. | (英) Ohta Ken-Ichi (日) (読)
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| 6. | (英) Kinoshita Hiroshi (日) (読)
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| ○題名 (必須): | □ | (英) Ethanol concentration induces production of 3,4-dihydroxyphenylacetic acid and homovanillic acid in mouse brain through activation of monoamine oxidase pathway. (日)
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| ○要約 (任意): | □ | (英) First, we aimed to investigate ex vivo the effects of ethanol (EtOH) on levels of norepinephrine (NE), dopamine (DA), serotonin (5-HT), and their metabolites in the frontal cortex, hippocampus, and striatum of Aldh2-knockout (Aldh2-KO) and wild-type (WT) mice. Animals were treated intraperitoneally with saline (control) or EtOH (1.0, 2.0, or 3.0g/kg). Brain samples were collected 60 and 120min after EtOH injection, and monoamines and their metabolites were measured by HPLC-ECD. We found in both WT and Aldh2-KO mice that 3.0g/kg EtOH increased the levels of 3,4-dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA) and decreased the level of 3-methoxytyramine (3-MT). A 2.0g/kg dose of EtOH also increased HVA, but there was not a consistent effect within the brain regions of Aldh2-KO and WT mice. There were inconsistent findings of genotype differences in the levels of DA, 5-HT, and their metabolites in the brain regions tested. None of the EtOH doses altered NE, DA, 5-HT, or 5-hydroxyindoleacetic acid contents in any of the brain regions studied. Second, we tested whether EtOH-induced increases in DOPAC and HVA are mediated by increased monoamine oxidase (MAO) or catechol-O-methyltransferase (COMT) activity. To test this, we used the MAO blocker clorgyline (2.0 and 4.0mg/kg) and the COMT blocker tolcapone (15 and 30mg/kg) alone or in combination with EtOH (3.0g/kg). Clorgyline alone increased 3-MT and decreased DOPAC and HVA levels, whereas tolcapone alone increased DOPAC and decreased 3-MT and HVA levels. Surprisingly, the combination of EtOH with clorgyline (4.0mg/kg) or tolcapone (30mg/kg) further decreased 3-MT and increased DOPAC and HVA levels, an effect that reversed the inhibitor-induced decreases in HVA. These results suggest that a high concentration of EtOH can accelerate DA metabolism, as evidenced by the increase in DOPAC and HVA, and this effect is likely a consequence of increased degradation of DA by MAO. (日)
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| ○キーワード (推奨): | 1. | (英) 3,4-Dihydroxyphenylacetic Acid (日) (読)
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| 2. | (英) Aldehyde Dehydrogenase, Mitochondrial (日) (読)
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| 3. | (英) Animals (日) (読)
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| 4. | (英) Brain (日) (読)
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| 5. | (英) Catechol O-Methyltransferase (日) (読)
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| 6. | (英) Clorgyline (日) (読)
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| 7. | (英) Ethanol (日) (読)
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| 8. | (英) Homovanillic Acid (日) (読)
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| 9. | (英) Mice (日) (読)
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| 10. | (英) Monoamine Oxidase (日) (読)
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| 11. | (英) Norepinephrine (日) (読)
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| 12. | (英) Serotonin (日) (読)
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| 13. | (英) Tolcapone (日) (読)
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| ○発行所 (推奨): |
| ○誌名 (必須): | □ | Neuroscience Letters ([Elsevier Science])
(pISSN: 0304-3940, eISSN: 1872-7972)
| ○ISSN (任意): | □ | 1872-7972
ISSN: 0304-3940
(pISSN: 0304-3940, eISSN: 1872-7972) Title: Neuroscience lettersTitle(ISO): Neurosci LettPublisher: Elsevier Ireland Ltd (NLM Catalog)
(Scopus)
(CrossRef)
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| ○巻 (必須): | □ | 782
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| ○号 (必須): |
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| ○年月日 (必須): | □ | 西暦 2022年 5月 19日 (令和 4年 5月 19日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1016/j.neulet.2022.136689 (→Scopusで検索)
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| ○PMID (任意): | □ | 35598694 (→Scopusで検索)
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| ○備考 (任意): | 1. | (英) PublicationType: Journal Article (日)
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| 2. | (英) PublicationType: Research Support, Non-U.S. Gov't (日)
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