| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○著者 (必須): | 1. | (英) Nishiguchi Akihiro (日) (読)
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| 2. | 村上 明一 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.基礎歯学系.口腔微生物学])
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| 3. | (英) Azuma Takachika (日) (読)
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| 4. | (英) Oda Masayuki (日) (読)
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| ○題名 (必須): | □ | (英) A Trade-off Between Thermostability and Binding Affinity of Anti-(4-hydroxy-3-nitrophenyl)Acetyl Antibodies During the Course of Affinity Maturation. (日)
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| ○要約 (任意): | □ | (英) Somatic hypermutation (SHM) is one of the driving forces that increases antibody (Ab) affinity. We studied the effects of SHM on thermostability and affinity using three single-chain Fv fragments (scFvs) of anti-(4-hydroxy-3-nitrophenyl)acetyl Abs, namely 9TG, 9T7, and E11. 9TG has a germline structure that lacks SHM and is an ancestor of 9T7 with 11 mutations. E11, which has 21 mutations, is a mature Ab and has its own ancestor. The thermostabilities and antigen-Ab interactions were analyzed by circular dichroism (CD), differential scanning calorimetry (DSC), and isothermal titration calorimetry (ITC). Far-UV CD spectra showed that all scFvs were folded into a structure referred to as immunoglobulin-fold and were unfolded by heating at different melting temperatures. Comparison of thermodynamic parameters obtained from DSC and ITC revealed that the magnitude of stabilization free energy at 37 °C was in the order, 9TG > 9T7 > E11, while that of the free energy of interaction with antigen was 9TG < 9T7 < E11, suggesting that Abs make a trade-off between stability and affinity during affinity maturation. (日)
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| ○キーワード (推奨): | 1. | (英) Antibodies (日) (読)
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| 2. | (英) Antibody Affinity (日) (読)
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| 3. | (英) Circular Dichroism (日) (読)
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| 4. | (英) Thermodynamics (日) (読)
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| ○発行所 (推奨): |
| ○誌名 (必須): | □ | The Protein Journal ([Springer])
(pISSN: 1572-3887, eISSN: 1875-8355)
| ○ISSN (任意): | □ | 1875-8355
ISSN: 1572-3887
(pISSN: 1572-3887, eISSN: 1875-8355) Title: The protein journalTitle(ISO): Protein JSupplier: Kluwer OnlinePublisher: Springer (NLM Catalog)
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| ○巻 (必須): | □ | 41
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| ○号 (必須): | □ | 2
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| ○頁 (必須): | □ | 293 303
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| ○年月日 (必須): | □ | 西暦 2022年 4月 30日 (令和 4年 4月 30日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1007/s10930-022-10053-w (→Scopusで検索)
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| ○PMID (任意): | □ | 35501455 (→Scopusで検索)
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| ○備考 (任意): | 1. | (英) PublicationType: Journal Article (日)
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| 2. | (英) PublicationType: Research Support, Non-U.S. Gov't (日)
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