| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○招待 (推奨): |
| ○審査 (推奨): | □ | Peer Review
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| ○カテゴリ (推奨): |
| ○共著種別 (推奨): | □ | 国内共著 (徳島大学内研究者と国内(学外)研究者との共同研究 (国外研究者を含まない))
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| ○学究種別 (推奨): |
| ○組織 (推奨): |
| ○著者 (必須): | 1. | 米田 晋太朗
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| ○学籍番号 (推奨): | □ | ****
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| 2. | 福田 達也
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| 3. | 大園 瑞音
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| 4. | 小暮 健太朗 ([徳島大学.大学院医歯薬学研究部.薬学域.薬科学部門.創薬科学系.衛生薬学])
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| ○学籍番号 (推奨): |
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| ○題名 (必須): | □ | (英) Enhancement of cerebroprotective effects of lipid nanoparticles encapsulating FK506 on cerebral ischemia/reperfusion injury by particle size regulation (日)
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| ○副題 (任意): |
| ○要約 (任意): | □ | (英) Delivery of cerebroprotective agents using liposomes has been demonstrated to be useful for treating cerebral ischemia/reperfusion (I/R) injury. We previously reported that intravenous administration of liposomes with diameters of 100 nm showed higher accumulation in the I/R region compared with larger liposomes (>200 nm) by passage through the disintegrated blood-brain barrier, suggesting a size-dependence for liposome-mediated drug delivery. Based on these findings, we hypothesized that regulation of liposomal particle size (<100 nm) may enhance the therapeutic efficacy of encapsulated drugs on cerebral I/R injury. Herein, we prepared lipid nanoparticles (LNP) with particle sizes <100 nm by the microfluidics method and compared their therapeutic potential with LNP exhibiting sizes >100 nm in cerebral I/R model rats. Intravenously administered smaller LNP (ca. 60 nm) exhibited wider accumulation and diffusivity in the brain parenchyma of the I/R region compared with larger LNP (>100 nm). Importantly, treatment with LNP encapsulating the cerebroprotective agent FK506 (FK-LNP) with particle sizes <100 nm showed greater cerebroprotective effects than FK-LNP with sizes >100 nm, and also significantly ameliorated brain injury. These results suggest that particle size regulation of LNP to sizes <100 nm can enhance the therapeutic effect of encapsulated drugs for treatment of cerebral I/R injury, and that FK-LNP could be a promising cerebroprotective agent. (日)
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| ○キーワード (推奨): | 1. | (英) Animals (日) (読)
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| 2. | (英) Brain Ischemia (日) (読)
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| 3. | (英) Liposomes (日) (読)
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| 4. | (英) Nanoparticles (日) (読)
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| 5. | (英) Neuroprotective Agents (日) (読)
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| 6. | (英) Particle Size (日) (読)
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| 7. | (英) Rats (日) (読)
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| 8. | (英) Rats, Wistar (日) (読)
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| 9. | (英) Reperfusion Injury (日) (読)
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| 10. | (英) Tacrolimus (日) (読)
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| ○発行所 (推奨): |
| ○誌名 (必須): | □ | Biochemical and Biophysical Research Communications ([Elsevier])
(pISSN: 0006-291X, eISSN: 1090-2104)
| ○ISSN (任意): | □ | 1090-2104
ISSN: 0006-291X
(pISSN: 0006-291X, eISSN: 1090-2104) Title: Biochemical and biophysical research communicationsTitle(ISO): Biochem Biophys Res CommunPublisher: Elsevier B.V. (NLM Catalog)
(Scopus)
(CrossRef)
(Scopus information is found. [need login]) | [継承] |
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| ○巻 (必須): | □ | 611
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| ○号 (必須): | □ |
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| ○頁 (必須): | □ | 53 59
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| ○都市 (任意): |
| ○年月日 (必須): | □ | 西暦 2022年 6月 30日 (令和 4年 6月 30日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1016/j.bbrc.2022.04.080 (→Scopusで検索)
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| ○PMID (任意): | □ | 35477093 (→Scopusで検索)
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| ○備考 (任意): | 1. | (英) Article.ELocationID: S0006-291X(22)00615-5 (日)
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| 2. | (英) Article.ELocationID: 10.1016/j.bbrc.2022.04.080 (日)
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| 3. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
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| 4. | (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't (日)
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| 5. | (英) KeywordList.Keyword: Blood-brain barrier (日)
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| 6. | (英) KeywordList.Keyword: Cerebral ischemia/reperfusion injury (日)
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| 7. | (英) KeywordList.Keyword: FK506 (日)
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| 8. | (英) KeywordList.Keyword: Lipid nanoparticles (日)
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| 9. | (英) KeywordList.Keyword: Microfluidics (日)
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| 10. | (英) KeywordList.Keyword: Particle size regulation (日)
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| 11. | (英) CoiStatement: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Tatsuya Fukuta reports financial support was provided by Takahashi Industrial and Economic Research Foundation. (日)
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