| ○種別 (必須): | □ | 学術論文 (紀要その他)
| [継承] |
| ○言語 (必須): | □ | 英語
| [継承] |
| ○招待 (推奨): |
| ○審査 (推奨): |
| ○カテゴリ (推奨): |
| ○共著種別 (推奨): |
| ○学究種別 (推奨): |
| ○組織 (推奨): |
| ○著者 (必須): | 1. | (英) Gao Luping (日) 高 露萍 (読) こう ろへい
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): | □ | ****
| [ユーザ] |
| [継承] |
| 2. | 森根 裕二 ([徳島大学.大学院医歯薬学研究部.医学域.医科学部門.外科系.消化器・移植外科学]/[徳島大学.病院.診療科.外科.消化器・移植外科])
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| 3. | 山田 眞一郎 ([徳島大学.病院.診療科.外科.消化器・移植外科])
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| 4. | 齋藤 裕 ([徳島大学.病院.先端医科医療開発研究プロジェクト]/[徳島大学.病院.診療科.外科.消化器・移植外科])
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| 5. | 池本 哲也 ([徳島大学.病院.中央診療施設等.安全管理部]/[徳島大学.病院.診療科.外科.消化器・移植外科])
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| 6. | 徳田 和憲 ([徳島大学.病院.診療科.第一外科])
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| 7. | 宮崎 克己 ([徳島大学.病院.診療科.第一外科])
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| 8. | (英) Okikawa Shouhei (日) (読)
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| 9. | 髙須 千絵 ([徳島大学.大学院医歯薬学研究部.医学域.医科学部門.外科系.消化器・移植外科学])
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| 10. | 島田 光生 ([徳島大学.大学院医歯薬学研究部.医学域.医科学部門.外科系.消化器・移植外科学])
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| ○題名 (必須): | □ | (英) The BAFF/NF B axis is crucial to interactions between sorafenib-resistant HCC cells and cancer-associated fibroblasts. (日)
| [継承] |
| ○副題 (任意): |
| ○要約 (任意): | □ | (英) The tumor microenvironment affects malignancy in hepatocellular carcinoma (HCC) cells, and cancer-associated fibroblasts (CAFs) play an important role in the microenvironment. As recent studies indicated a difference between CAFs isolated from chemoresistant and non-resistant cancer tissues, therefore we investigated the intracellular mechanism in resistant HCC co-cultured CAFs and interactions between these CAFs with cancer cells. We established a sorafenib-resistant (SR) Huh7 (human HCC) cell line, and characterized it with cytokine assays, then developed CAFs by co-culturing human hepatic stellate cells with resistant or parental Huh7 cells. The 2 types of CAFs were co-cultured with parental Huh7 cells, thereafter the cell viability of these Huh7 cells was checked under sorafenib treatment. The SR Huh7 (Huh7 ) cells expressed increased B-cell activating factor (BAFF), which promoted high expression of CAF-specific markers in Huh7 -co-cultured CAFs, showed activated BAFF, BAFF-R, and downstream of the NF B-Nrf2 pathway, and aggravated invasion, migration, and drug resistance in co-cultured Huh7 cells. When we knocked down BAFF expression in Huh7 cells, the previously increased malignancy and BAFF/NF B axis in Huh7 -co-cultured CAFs reversed, and enhanced chemoresistance in co-cultured Huh7 cells returned as well. In conclusion, the BAFF/NF B pathway was activated in CAFs co-cultured with cell-culture medium from resistant Huh7, which promoted chemoresistance, and increased the malignancy in co-cultured non-resistant Huh7 cells. This suggests that the BAFF/NF B axis in CAFs might be a potential therapeutic target in chemoresistance of HCC. (日)
| [継承] |
| ○キーワード (推奨): | 1. | (英) Antineoplastic Agents (日) (読)
| [継承] |
| 2. | (英) B-Cell Activating Factor (日) (読)
| [継承] |
| 3. | (英) Cancer-Associated Fibroblasts (日) (読)
| [継承] |
| 4. | (英) Carcinoma, Hepatocellular (日) (読)
| [継承] |
| 5. | (英) Cell Communication (日) (読)
| [継承] |
| 6. | (英) Cell Line, Tumor (日) (読)
| [継承] |
| 7. | (英) Cell Movement (日) (読)
| [継承] |
| 8. | (英) Cell Survival (日) (読)
| [継承] |
| 9. | (英) Coculture Techniques (日) (読)
| [継承] |
| 10. | (英) Drug Resistance, Neoplasm (日) (読)
| [継承] |
| 11. | (英) Gene Knockdown Techniques (日) (読)
| [継承] |
| 12. | (英) Humans (日) (読)
| [継承] |
| 13. | (英) Liver Neoplasms (日) (読)
| [継承] |
| 14. | (英) NF-kappa B (日) (読)
| [継承] |
| 15. | (英) Signal Transduction (日) (読)
| [継承] |
| 16. | (英) Sorafenib (日) (読)
| [継承] |
| 17. | (英) Transfection (日) (読)
| [継承] |
| ○発行所 (推奨): |
| ○誌名 (必須): | □ | Cancer Science ([日本癌学会])
(pISSN: 1347-9032, eISSN: 1349-7006)
| ○ISSN (任意): | □ | 1349-7006
ISSN: 1347-9032
(pISSN: 1347-9032, eISSN: 1349-7006) Title: Cancer scienceTitle(ISO): Cancer SciPublisher: Wiley-Blackwell (NLM Catalog)
(医中誌Web)
(Scopus)
(CrossRef)
(Scopus information is found. [need login]) | [継承] |
| [継承] |
| ○巻 (必須): | □ | 112
| [継承] |
| ○号 (必須): | □ | 9
| [継承] |
| ○頁 (必須): | □ | 3545 3554
| [継承] |
| ○都市 (任意): |
| ○年月日 (必須): | □ | 西暦 2021年 7月 16日 (令和 3年 7月 16日)
| [継承] |
| ○URL (任意): |
| ○DOI (任意): | □ | 10.1111/cas.15041 (→Scopusで検索)
| [継承] |
| ○PMID (任意): | □ | 34159680 (→Scopusで検索)
| [継承] |
| ○CRID (任意): |
| ○WOS (任意): |
| ○Scopus (任意): |
| ○評価値 (任意): |
| ○被引用数 (任意): |
| ○指導教員 (推奨): |
| ○備考 (任意): | 1. | (英) Article.ELocationID: 10.1111/cas.15041 (日)
| [継承] |
| 2. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
| [継承] |
| 3. | (英) KeywordList.Keyword: B-cell activating factor (日)
| [継承] |
| 4. | (英) KeywordList.Keyword: cancer-associated fibroblasts (日)
| [継承] |
| 5. | (英) KeywordList.Keyword: drug resistance (日)
| [継承] |
| 6. | (英) KeywordList.Keyword: hepatocellular carcinoma (日)
| [継承] |
| 7. | (英) KeywordList.Keyword: sorafenib (日)
| [継承] |