| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○招待 (推奨): |
| ○審査 (推奨): | □ | Peer Review
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| ○カテゴリ (推奨): | □ | 研究
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| ○共著種別 (推奨): |
| ○学究種別 (推奨): |
| ○組織 (推奨): | 1. | 徳島大学.大学院社会産業理工学研究部.生物資源産業学域.応用生命系.応用生物資源学分野 (2017年4月1日〜2022年3月31日)
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| ○著者 (必須): | 1. | 白井 昭博 ([徳島大学.大学院社会産業理工学研究部.生物資源産業学域.応用生命系.応用生物資源学分野]/[徳島大学.生物資源産業学部.生物資源産業学科.応用生命コース])
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| 2. | (英) Kawasaka Kaito (日) (読)
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| ○学籍番号 (推奨): | □ | ****
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| 3. | 土屋 浩一郎 ([徳島大学.大学院医歯薬学研究部.薬学域.薬科学部門.生命薬学系.医薬品機能生化学]/[徳島大学.医学部.医学科.病態情報医学講座])
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| ○題名 (必須): | □ | (英) Antimicrobial action of phenolic acids combined with violet 405-nm light for disinfecting pathogenic and spoilage fungi (日)
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| ○副題 (任意): |
| ○要約 (任意): | □ | (英) The aim of this study is to investigate the fungicidal spectrum of six phenolic-cinnamic and -benzoic acid derivatives using four fungi, Aspergillus niger, Cladosporium cladosporioides, Trichophyton mentagrophytes and Candida albicans, in a photocombination system with violet 405-nm light. This is the first study to examine the fungicidal mechanism involving oxidative damage using the conidium of A. niger, as well as an assessment of cellular function and chemical characteristics. The results of the screening assay indicated that ferulic acid (FA) and vanillic acid (VA), which possess 4-hydroxyl and 3-methoxy groups in their phenolic acid structures, produced synergistic activity with 405-nm light irradiation. FA and VA (5.0 mM) significantly decreased the viability of A. niger by 2.4 to 2.6-logs under 90-min irradiation. The synergistic effects were attenuated by the addition of the radical scavenger dimethyl sulfoxide. Generation of reactive oxygen species (ROS), such as hydrogen peroxide and hydroxyl radicals, were confirmed in the phenolic acid solutions tested after irradiation with colorimetric and electron spin resonance analyses. Adsorption of FA and VA to conidia was greater than other tested phenolic acids, and produced 1.55- and 1.85-fold elevation of intracellular ROS levels, as determined using an oxidant-sensitive probe with flow cytometry analysis. However, cell wall or membrane damage was not the main mechanism by which the combination-induced fungal death was mediated. Intracellular ATP was drastically diminished (5% of control levels) following combined treatment with FA and light exposure, even under a condition that produced negligible decreases in viability, thereby resulting in pronounced growth delay. These results suggest that the first stage in the photofungicidal mechanism is oxidative damage to mitochondria or the cellular catabolism system associated with ATP synthesis, which is a result of the photoreaction of phenolic acids adsorbed and internalized by conidia. This photo-technology in combination with food-grade phenolic acids can aid in developing alternative approaches for disinfection of pathogenic and spoilage fungi in the fields of agriculture, food processing and medical care. (日)
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| ○キーワード (推奨): | 1. | (英) Synergistic antifungal activity (日) (読)
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| 2. | (英) Fungicidal action (日) (読)
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| 3. | (英) Phenolic acid (日) (読)
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| 4. | (英) Violet 405-nm light (日) (読)
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| 5. | (英) Oxidative stress (日) (読)
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| 6. | (英) Growth delay (日) (読)
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| ○発行所 (推奨): | □ | Elsevier (->組織[Elsevier Science])
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| ○誌名 (必須): | □ | Journal of Photochemistry and Photobiology B: Biology ([Elsevier])
(pISSN: 1011-1344, eISSN: 1873-2682)
| ○ISSN (任意): | □ | 1873-2682
ISSN: 1011-1344
(pISSN: 1011-1344, eISSN: 1873-2682) Title: Journal of photochemistry and photobiology. B, BiologyTitle(ISO): J Photochem Photobiol BPublisher: Elsevier B.V. (NLM Catalog)
(Scopus)
(CrossRef)
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| ○巻 (必須): | □ | 229
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| ○号 (必須): | □ |
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| ○頁 (必須): | □ | 112411 112411
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| ○都市 (任意): |
| ○年月日 (必須): | □ | 西暦 2022年 4月 5日 (令和 4年 4月 5日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1016/j.jphotobiol.2022.112411 (→Scopusで検索)
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| ○PMID (任意): | □ | 35219030 (→Scopusで検索)
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| ○CRID (任意): |
| ○Scopus (任意): | 1. | 2-s2.0-85125124978
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| ○備考 (任意): | 1. | (英) Article.ELocationID: 10.1016/j.jphotobiol.2013.11.027 (日)
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| 2. | (英) Article.ELocationID: S1011-1344(13)00279-0 (日)
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| 3. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
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| 4. | (英) KeywordList.Keyword: Bactericidal action (日)
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| 5. | (英) KeywordList.Keyword: Disinfection (日)
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| 6. | (英) KeywordList.Keyword: Gemini-quaternary ammonium salt (日)
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| 7. | (英) KeywordList.Keyword: Reactive oxygen species (日)
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| 8. | (英) KeywordList.Keyword: Ultraviolet-A (日)
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