| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○招待 (推奨): |
| ○審査 (推奨): |
| ○カテゴリ (推奨): |
| ○共著種別 (推奨): | □ | 国内共著 (徳島大学内研究者と国内(学外)研究者との共同研究 (国外研究者を含まない))
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| ○学究種別 (推奨): |
| ○組織 (推奨): |
| ○著者 (必須): | 1. | (英) Nakamura Kazuya (日) 中村 和也 (読) なかむら かずや
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| ○学籍番号 (推奨): | □ | ****
| [ユーザ] |
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| 2. | (英) Aihara Keisuke (日) (読)
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| 3. | 石田 竜弘 ([徳島大学.大学院医歯薬学研究部.薬学域.薬科学部門.生命薬学系.薬物動態制御学]/[徳島大学.大学院医歯薬学研究部.薬学域.連携研究部門(薬学域).寄附講座系(薬学域).がん細胞と代謝学]/[徳島大学.大学産業院])
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| ○題名 (必須): | □ | (英) Importance of process parameters influencing the mean diameters of siRNA-containing lipid nanoparticles (LNPs) on the in vitro activity of prepared LNPs (日)
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| ○副題 (任意): |
| ○要約 (任意): | □ | (英) Genetic drugs have the potential to treat a variety of diseases. Recently, lipid nanoparticles (LNPs) have attracted much attention among drug delivery systems for genetic drugs. LNPs have been practically used in small interfering RNA (siRNA) drugs and mRNA vaccines. Although LNPs are generally prepared by mixing nucleic acids in acidic aqueous buffer and lipid excipients in alcohol (i.e., ethanol), it is not well understood which process parameters in the LNPs formation affect the physicochemical properties and the functionality of LNPs. In this study, we used siRNA-containing LNPs as a model, and evaluated the effect that aqueous solution parameters (buffering agent type, salt concentration, and pH) and mixing parameters (ratio, speed, and temperature) exert on the physicochemical properties and in vitro gene-knockdown activity of LNPs. Among such parameters, the type of buffering agent, salt concentration (ionic strength), pH in acidic aqueous buffer, as well as the mixing ratio and speed significantly affected the mean particle diameter and in vitro gene-knockdown activity of LNPs. A strong correlation between the mean particle diameters and their in vitro gene-knockdown activities was observed. These observations suggest that the process parameters influencing the mean LNPs diameter are likely to be important in the formation of LNPs and also that these correlate with in vitro gene-knockdown activity. Because LNP systems are being further developed for future clinical applications of genetic drugs, information regarding the LNPs manufacturing process is of utmost importance. The results observed in this study will be useful for the manufacturing of optimal LNPs. (日)
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| ○キーワード (推奨): | 1. | (英) Lipids (日) (読)
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| 2. | (英) Liposomes (日) (読)
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| 3. | (英) Nanoparticles (日) (読)
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| 4. | (英) RNA, Small Interfering (日) (読)
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| ○発行所 (推奨): |
| ○誌名 (必須): | □ | Biological & Pharmaceutical Bulletin ([日本薬学会])
(pISSN: 0918-6158, eISSN: 1347-5215)
| ○ISSN (任意): | □ | 1347-5215
ISSN: 0918-6158
(pISSN: 0918-6158, eISSN: 1347-5215) Title: Biological & pharmaceutical bulletinTitle(ISO): Biol Pharm BullSupplier: 公益社団法人日本薬学会Publisher: Pharmaceutical Society of Japan (NLM Catalog)
(CiNii NCID)
(医中誌Web)
(J-STAGE)
(Scopus)
(CrossRef)
(Scopus information is found. [need login]) | [継承] |
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| ○巻 (必須): | □ | 45
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| ○号 (必須): | □ | 4
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| ○頁 (必須): | □ | 497 507
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| ○都市 (任意): |
| ○年月日 (必須): | □ | 西暦 2022年 4月 1日 (令和 4年 4月 1日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1248/bpb.b21-01016 (→Scopusで検索)
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| ○PMID (任意): | □ | 35370275 (→Scopusで検索)
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| ○備考 (任意): | 1. | (英) Article.ELocationID: 10.1248/bpb.b21-01016 (日)
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| 2. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
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| 3. | (英) KeywordList.Keyword: lipid nanoparticle (日)
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| 4. | (英) KeywordList.Keyword: mRNA (日)
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| 5. | (英) KeywordList.Keyword: manufacture (日)
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| 6. | (英) KeywordList.Keyword: process parameter (日)
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| 7. | (英) KeywordList.Keyword: small interfering RNA (siRNA) (日)
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