| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○招待 (推奨): |
| ○審査 (推奨): | □ | Peer Review
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| ○カテゴリ (推奨): | □ | 研究
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| ○共著種別 (推奨): |
| ○学究種別 (推奨): |
| ○組織 (推奨): | 1. | 徳島大学.大学院社会産業理工学研究部.生物資源産業学域.応用生命系.応用生物資源学分野 (2017年4月1日〜2022年3月31日)
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| ○著者 (必須): | 1. | 白井 昭博 ([徳島大学.大学院社会産業理工学研究部.生物資源産業学域.応用生命系.応用生物資源学分野]/[徳島大学.生物資源産業学部.生物資源産業学科.応用生命コース])
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| 2. | (英) Kunimi Haruka (日) (読)
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| ○学籍番号 (推奨): | □ | ****
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| 3. | 土屋 浩一郎 ([徳島大学.大学院医歯薬学研究部.薬学域.薬科学部門.生命薬学系.医薬品機能生化学]/[徳島大学.医学部.医学科.病態情報医学講座])
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| ○題名 (必須): | □ | (英) Antifungal action of the combination of ferulic acid and ultraviolet-A irradiation against Saccharomyces cerevisiae (日)
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| ○要約 (任意): | □ | (英) Aims To examine the antifungal action of photocombination treatment with ferulic acid (FA) and ultraviolet-A (UV-A) light (wavelength, 365 nm) by investigating associated changes in cellular functions of Saccharomyces cerevisiae. Methods and Results When pre-incubation of yeast cells with FA was extended from 0.5 to 10 min, its photofungicidal activity increased. Flow cytometry analysis of stained live and dead cells revealed that 10-min UV-A exposure combined with FA (1 mg ml-1) induced a ~ 99.9% decrease in cell viability although maintaining cell membrane integrity when compared with pre-exposure samples. When morphological and biochemical analysis were performed, treated cells exhibited an intact cell surface and oxidative DNA damage similar to control cells. Photocombination treatment induced cellular proteins oxidation, as shown by 2.3-fold increasing in immunostaining levels of ~49-kDa carbonylated proteins compared with pre-irradiation samples. Pyruvate kinase 1 (PK1) was identified by proteomics analysis as a candidate protein whose levels was affected by photocombination treatment. Moreover, intracellular ATP levels decreased following FA treatment both in darkness and with UV-A irradiation, thus suggesting a possible FA-induced delay in cell growth. Conclusions FA functions within the cytoplasmic membrane; addition of UV-A exposure induces increased oxidative modifications of cytosolic proteins such as PK1, which functions in ATP generation, without causing detectable genotoxicity, thus triggering inactivation of yeast cells. Significance and Impact of Study Microbial contamination is a serious problem that diminishes the quality of fruits and vegetables. Combining light exposure with food-grade phenolic acids such as FA is a promising disinfection technology for applications in agriculture and food processing. However, the mode of photofungicidal action of FA with UV-A light remains unclear. This study is the first to elucidate the mechanism using S. cerevisiae. Moreover, proteomics analyses identified a specific cytosolic protein, PK1, which is oxidatively modified by photocombination treatment. (日)
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| ○キーワード (推奨): | 1. | (英) Antifungal action (日) (読)
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| 2. | (英) Ferulic acid (日) (読)
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| 3. | (英) Ultraviolet-A (日) (読)
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| 4. | (英) Synergism (日) (読)
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| 5. | (英) Oxidative stress (日) (読)
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| 6. | (英) Pyruvate kinase 1 (日) (読)
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| ○発行所 (推奨): | □ | John Wiley & Sons, Inc.
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| ○誌名 (必須): | □ | Journal of Applied Microbiology (Society for Applied Bacteriology/Society for Applied Microbiology)
(pISSN: 1364-5072, eISSN: 1365-2672)
| ○ISSN (任意): | □ | 1365-2672
ISSN: 1364-5072
(pISSN: 1364-5072, eISSN: 1365-2672) Title: Journal of applied microbiologyTitle(ISO): J Appl MicrobiolSupplier: Society for Applied MicrobiologyPublisher: Oxford University Press (NLM Catalog)
(Wiley)
(Scopus)
(CrossRef)
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| ○巻 (必須): | □ | 132
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| ○号 (必須): | □ | 4
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| ○頁 (必須): | □ | 2957 2967
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| ○年月日 (必須): | □ | 西暦 2022年 4月 初日 (令和 4年 4月 初日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1111/jam.15407 (→Scopusで検索)
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| ○PMID (任意): | □ | 34894031 (→Scopusで検索)
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| ○備考 (任意): | 1. | (英) Article.ELocationID: 10.1016/j.jphotobiol.2013.11.027 (日)
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| 2. | (英) Article.ELocationID: S1011-1344(13)00279-0 (日)
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| 3. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
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| 4. | (英) KeywordList.Keyword: Bactericidal action (日)
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| 5. | (英) KeywordList.Keyword: Disinfection (日)
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| 6. | (英) KeywordList.Keyword: Gemini-quaternary ammonium salt (日)
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| 7. | (英) KeywordList.Keyword: Reactive oxygen species (日)
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| 8. | (英) KeywordList.Keyword: Ultraviolet-A (日)
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