『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=359322EID:359322, Map:0, LastModified:2019年9月7日(土) 21:12:15, Operator:[大家 隆弘], Avail:TRUE, Censor:0, Owner:[堤 理恵], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
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審査 (推奨):
カテゴリ (推奨):
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組織 (推奨):
著者 (必須): 1. (英) Yasuoka Susumu (日) (読)
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2. (英) Miki Mari (日) (読)
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3.堤 理恵 ([徳島大学.大学院医歯薬学研究部.医学域.栄養科学部門.医科栄養学系.代謝栄養学])
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4.吉岡 昌美
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5. (英) Bando Takashi (日) (読)
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6. (英) Michishige Fumiko (日) (読)
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題名 (必須): (英) Human airway trypsin-like protease (HAT) is released into saliva.  (日)    [継承]
副題 (任意):
要約 (任意): (英)   (日) We first discovered human airway trypsin-like protease (HAT) in human mucoid sputum. Precursor HAT (47 kDa), a cell surface type Ⅱ transmembrane serine protease, is proteolyzed to mature HAT (27 kDa). Hitherto, HAT has not been detected in other biological fluids except for human sputum. We aimed to clarify whether human saliva contains mature HAT. Trypsin-like protease was isolated from saliva of healthy volunteers by a method adopted for isolation of HAT from sputum using Boc-Phe-Ser-Arg-MCA as the substrate. Biochemical properties of purified protease were similar to those of recombinant HAT (rHAT). HAT concentration in saliva was measured by ELISA, and immunoreactive HAT:total protein ratio (ng/mg) in saliva samples from healthy subjects was similar to that in mucoid sputum. RT-PCR showed that HAT mRNA was expressed in human gingival epithelial cells but not in gingival fibroblasts. Both indirect immunofluorescence and western blotting using monoclonal antibody for α-smooth muscle actin (α-SMA;a myofibroblast marker) showed that HAT enhanced α-SMA fiber expression in gingival fibroblasts. These results indicate that both mucoid sputum and saliva from healthy subjects have similar concentrations of mature HAT, and HAT is related to certain physiological functions and pathological states of myofibroblasts in the oral cavity. J. Med. Invest. 65:258-267, August, 2018.   [継承]
キーワード (推奨): 1. (英) Actins (日) (読) [継承]
2. (英) Adult (日) (読) [継承]
3. (英) Cells, Cultured (日) (読) [継承]
4. (英) Epithelial Cells (日) (読) [継承]
5. (英) Female (日) (読) [継承]
6. (英) Fibroblasts (日) (読) [継承]
7. (英) Gingiva (日) (読) [継承]
8. (英) Healthy Volunteers (日) (読) [継承]
9. (英) Humans (日) (読) [継承]
10. (英) Middle Aged (日) (読) [継承]
11. (英) RNA, Messenger (日) (読) [継承]
12. (英) Saliva (日) (読) [継承]
13. (英) Serine Endopeptidases (日) (読) [継承]
14. (英) Sputum (日) (読) [継承]
15. (英) Young Adult (日) (読) [継承]
発行所 (推奨):
誌名 (必須): The Journal of Medical Investigation : JMI ([徳島大学.医学部])
(pISSN: 1343-1420, eISSN: 1349-6867)

ISSN (任意): 1349-6867
ISSN: 1343-1420 (pISSN: 1343-1420, eISSN: 1349-6867)
Title: The journal of medical investigation : JMI
Title(ISO): J Med Invest
Supplier: 徳島大学
Publisher: Tokushima Daigaku Igakubu
 (NLM Catalog  (Webcat Plus  (医中誌Web  (J-STAGE  (Scopus  (CrossRef (Scopus information is found. [need login])
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年月日 (必須): 西暦 2018年 0月 初日 (平成 30年 0月 初日) [継承]
URL (任意):
DOI (任意): 10.2152/jmi.65.258    (→Scopusで検索) [継承]
PMID (任意): 30282870    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) PublicationType: Journal Article  (日)    [継承]

標準的な表示

和文冊子 ● Susumu Yasuoka, Mari Miki, Rie Tsutsumi, Masami Yoshioka, Takashi Bando and Fumiko Michishige : Human airway trypsin-like protease (HAT) is released into saliva., The Journal of Medical Investigation : JMI, Vol.65, No.3.4, 258-267, 2018.
欧文冊子 ● Susumu Yasuoka, Mari Miki, Rie Tsutsumi, Masami Yoshioka, Takashi Bando and Fumiko Michishige : Human airway trypsin-like protease (HAT) is released into saliva., The Journal of Medical Investigation : JMI, Vol.65, No.3.4, 258-267, 2018.

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