| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○審査 (推奨): |
| ○カテゴリ (推奨): |
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| ○学究種別 (推奨): |
| ○組織 (推奨): |
| ○著者 (必須): | 1. | (英) Nuka Erika (日) 額 惠理香 (読) ぬか えりか
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| ○学籍番号 (推奨): | □ | ****
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| 2. | 大西 康太
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| 3. | 寺尾 純二
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| 4. | 河合 慶親
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| ○学籍番号 (推奨): |
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| ○題名 (必須): | □ | (英) ATP/P2X7 receptor signaling as a potential anti-inflammatory target of natural polyphenols. (日)
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| ○副題 (任意): |
| ○要約 (任意): | □ | (英) Innate immune cells, such as macrophages, respond to pathogen-associated molecular patterns, such as a lipopolysaccharide (LPS), to secrete various inflammatory mediators. Recent studies have suggested that damage-associated molecular patterns (DAMPs), released extracellularly from damaged or immune cells, also play a role in the activation of inflammatory responses. In this study, to prevent excess inflammation, we focused on DAMPs-mediated signaling that promotes LPS-stimulated inflammatory responses, especially adenosine 5'-triphosphate (ATP)-triggered signaling through the ionotropic purinergic receptor 7 (P2X7R), as a potential new anti-inflammatory target of natural polyphenols. We focused on the phenomenon that ATP accelerates the production of inflammatory mediators, such as nitric oxide, in LPS-stimulated J774.1 mouse macrophages. Using an siRNA-mediated knockdown and specific antagonist, it was found that the ATP-induced enhanced inflammatory responses were mediated through P2X7R. We then screened 42 polyphenols for inhibiting the ATP/P2X7R-induced calcium influx, and found that several polyphenols exhibited significant inhibitory effects. Especially, a flavonoid baicalein significantly inhibited ATP-induced inflammation, including interleukin-1β secretion, through inhibition of the ATP/P2X7R signaling. These findings suggest that ATP/P2X7R signaling plays an important role in excess inflammatory responses and could be a potential anti-inflammatory target of natural polyphenolic compounds. (日)
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| ○キーワード (推奨): | 1. | (英) Adenosine Triphosphate (日) (読)
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| 2. | (英) Animals (日) (読)
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| 3. | (英) Anti-Inflammatory Agents (日) (読)
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| 4. | (英) Biological Products (日) (読)
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| 5. | (英) Cell Line (日) (読)
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| 6. | (英) Drug Evaluation, Preclinical (日) (読)
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| 7. | (英) Flavanones (日) (読)
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| 8. | (英) Interleukin-1beta (日) (読)
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| 9. | (英) Lipopolysaccharides (日) (読)
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| 10. | (英) Macrophages (日) (読)
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| 11. | (英) Mice (日) (読)
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| 12. | (英) Nitric Oxide (日) (読)
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| 13. | (英) Polyphenols (日) (読)
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| 14. | (英) Receptors, Purinergic P2X7 (日) (読)
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| 15. | (英) Signal Transduction (日) (読)
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| ○発行所 (推奨): |
| ○誌名 (必須): | □ | PLoS ONE (Public Library of Science)
(eISSN: 1932-6203)
| ○ISSN (任意): | □ | 1932-6203
ISSN: 1932-6203
(eISSN: 1932-6203) Title: PloS oneTitle(ISO): PLoS OnePublisher: PLOS (NLM Catalog)
(Scopus)
(CrossRef)
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| ○巻 (必須): | □ | 13
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| ○号 (必須): | □ | 9
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| ○頁 (必須): | □ | e0204229 e0204229
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| ○都市 (任意): |
| ○年月日 (必須): | □ | 西暦 2018年 9月 24日 (平成 30年 9月 24日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1371/journal.pone.0204229 (→Scopusで検索)
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| ○PMID (任意): | □ | 30248132 (→Scopusで検索)
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| ○備考 (任意): | 1. | (英) Article.ELocationID: 10.1371/journal.pone.0204229 (日)
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| 2. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
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| 3. | (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't (日)
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| 4. | (英) CoiStatement: The authors have declared that no competing interests exist. (日)
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