| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○招待 (推奨): |
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| ○カテゴリ (推奨): |
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| ○学究種別 (推奨): |
| ○組織 (推奨): |
| ○著者 (必須): | 1. | (英) Hayashi Hideki (日) (読)
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| 2. | (英) Kubo Yoshinao (日) (読)
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| 3. | (英) Izumida Mai (日) (読)
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| 4. | (英) Takahashi Etsuhisa (日) (読)
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| 5. | 木戸 博 ([徳島大学.先端酵素学研究所.重点研究部門])
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| 6. | (英) Sato Ko (日) (読)
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| 7. | (英) Yamaya Mutsuo (日) (読)
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| 8. | (英) Nishimura Hidekazu (日) (読)
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| 9. | (英) Nakayama Kou (日) (読)
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| 10. | (英) Matsuyama Toshifumi (日) (読)
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| ○題名 (必須): | □ | (英) Enterokinase enhances influenza A virus infection by activating trypsinogen in human cell lines. (日)
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| ○副題 (任意): |
| ○要約 (任意): | □ | (英) Cleavage and activation of hemagglutinin (HA) by trypsin-like proteases in influenza A virus (IAV) are essential prerequisites for its successful infection and spread. In host cells, some transmembrane serine proteases such as TMPRSS2, TMPRSS4 and HAT, along with plasmin in the bloodstream, have been reported to cleave the HA precursor (HA) molecule into its active forms, HA and HA. Some trypsinogens can also enhance IAV proliferation in some cell types (e.g., rat cardiomyoblasts). However, the precise activation mechanism for this process is unclear, because the expression level of the physiological activator of the trypsinogens, the TMPRSS15 enterokinase, is expected to be very low in such cells, with the exception of duodenal cells. Here, we show that at least two variant enterokinases are expressed in various human cell lines, including A549 lung-derived cells. The exogenous expression of these enterokinases was able to enhance the proliferation of IAV in 293T human kidney cells, but the proliferation was reduced by knocking down the endogenous enterokinase in A549 cells. The enterokinase was able to enhance HA processing in the cells, which activated trypsinogen and in the IAV-infected cells also. Therefore, we conclude that enterokinase plays a role in IAV infection and proliferation by activating trypsinogen to process viral HA in human cell lines. (日)
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| ○キーワード (推奨): | 1. | (英) Cell Line (日) (読)
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| 2. | (英) Enzyme Activation (日) (読)
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| 3. | (英) Hemagglutinin Glycoproteins, Influenza Virus (日) (読)
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| 4. | (英) Host-Pathogen Interactions (日) (読)
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| 5. | (英) Humans (日) (読)
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| 6. | (英) Influenza A Virus, H1N1 Subtype (日) (読)
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| 7. | (英) Influenza, Human (日) (読)
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| 8. | (英) Protein Processing, Post-Translational (日) (読)
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| 9. | (英) Serine Endopeptidases (日) (読)
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| 10. | トリプシン (trypsin)
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| ○発行所 (推奨): |
| ○誌名 (必須): | □ | Frontiers in Cellular and Infection Microbiology (Frontiers Media)
(eISSN: 2235-2988)
| ○ISSN (任意): | □ | 2235-2988
ISSN: 2235-2988
(eISSN: 2235-2988) Title: Frontiers in cellular and infection microbiologyTitle(ISO): Front Cell Infect MicrobiolPublisher: Frontiers Media SA (NLM Catalog)
(Scopus)
(CrossRef)
(Scopus information is found. [need login]) | [継承] |
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| ○巻 (必須): | □ | 8
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| ○号 (必須): | □ |
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| ○頁 (必須): | □ | 91 91
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| ○都市 (任意): |
| ○年月日 (必須): | □ | 西暦 2018年 3月 23日 (平成 30年 3月 23日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.3389/fcimb.2018.00091 (→Scopusで検索)
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| ○PMID (任意): | □ | 29629340 (→Scopusで検索)
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| ○備考 (任意): | 1. | (英) Article.ELocationID: 10.3389/fcimb.2018.00091 (日)
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| 2. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
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| 3. | (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't (日)
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| 4. | (英) KeywordList.Keyword: enterokinase (日)
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| 5. | (英) KeywordList.Keyword: genome structure and function (日)
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| 6. | (英) KeywordList.Keyword: hemagglutinin processing (日)
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| 7. | (英) KeywordList.Keyword: influenza A virus (日)
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| 8. | (英) KeywordList.Keyword: transmembrane serine protease (日)
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