| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○カテゴリ (推奨): |
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| ○学究種別 (推奨): |
| ○組織 (推奨): |
| ○著者 (必須): | 1. | (英) Kim Hyejin (日) (読)
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| 2. | (英) Kimoto Takashi (日) (読)
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| 3. | (英) Sakai Satoko (日) (読)
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| 4. | (英) Takahashi Etsuhisa (日) (読)
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| 5. | 木戸 博 ([徳島大学.先端酵素学研究所.重点研究部門])
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| ○題名 (必須): | □ | (英) Adjuvanting influenza hemagglutinin vaccine with a human pulmonary surfactant-mimicking synthetic compound SF-10 induces local and systemic cell-mediated immunity in mice. (日)
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| ○副題 (任意): |
| ○要約 (任意): | □ | (英) We reported previously that intranasal instillation of a synthetic human pulmonary surfactant with a carboxy vinyl polymer as a viscosity improver, named SF-10, shows potent adjuvanticity for humoral immunity in mice and cynomolgus monkeys. SF-10 effectively induces influenza hemagglutinin vaccine (HAv)-specific IgA in nasal and lung washes and IgG in sera with their neutralizing activities. Since CD8+ T cell-mediated protection is an important requirement for adaptive immunity, we investigated in this study the effects of SF-10 with antigen on local and systemic cell-mediated immunity. Nasal instillation of ovalbumin, a model antigen, combined with SF-10 efficiently delivered antigen to mucosal dendritic and epithelial cells and promoted cross-presentation in antigen presenting cells, yielding a high percentage of ovalbumin-specific cytotoxic T lymphocytes in the nasal mucosa, compared with ovalbumin alone. Nasal immunization of HAv-SF-10 also induced HAv-specific cytotoxic T lymphocytes and upregulated granzyme B expression in splenic CD8+ T cells with their high cytotoxicity against target cells pulsed with HA peptide. Furthermore, nasal vaccination of HAv-SF-10 significantly induced higher cytotoxic T lymphocytes-mediated cytotoxicity in the lungs and cervical lymph nodes in the early phase of influenza virus infection compared with HAv alone. Protective immunity induced by HAv-SF-10 against lethal influenza virus infection was partially and predominantly suppressed after depletion of CD8+ and CD4+ T cells (induced by intraperitoneal injection of the corresponding antibodies), respectively, suggesting that CD4+ T cells predominantly and CD8+ T cells partially contribute to the protective immunity in the advanced stage of influenza virus infection. These results suggest that SF-10 promotes effective antigen delivery to antigen presenting cells, activates CD8+ T cells via cross-presentation, and induces cell-mediated immune responses against antigen. (日)
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| ○キーワード (推奨): | 1. | (英) Adjuvants, Immunologic (日) (読)
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| 2. | (英) Animals (日) (読)
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| 3. | (英) CD4-Positive T-Lymphocytes (日) (読)
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| 4. | (英) CD8-Positive T-Lymphocytes (日) (読)
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| 5. | 女性 (female)
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| 6. | (英) Hemagglutination, Viral (日) (読)
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| 7. | (英) Immunity, Cellular (日) (読)
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| 8. | (英) Influenza Vaccines (日) (読)
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| 9. | (英) Lymphocyte Depletion (日) (読)
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| 10. | (英) Mice (日) (読)
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| 11. | (英) Mice, Inbred BALB C (日) (読)
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| 12. | (英) Mice, Inbred C57BL (日) (読)
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| 13. | (英) Molecular Mimicry (日) (読)
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| 14. | (英) Nasal Mucosa (日) (読)
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| 15. | (英) Pulmonary Surfactants (日) (読)
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| ○発行所 (推奨): |
| ○誌名 (必須): | □ | PLoS ONE (Public Library of Science)
(eISSN: 1932-6203)
| ○ISSN (任意): | □ | 1932-6203
ISSN: 1932-6203
(eISSN: 1932-6203) Title: PloS oneTitle(ISO): PLoS OnePublisher: PLOS (NLM Catalog)
(Scopus)
(CrossRef)
(Scopus information is found. [need login]) | [継承] |
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| ○巻 (必須): | □ | 13
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| ○号 (必須): | □ | 1
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| ○頁 (必須): | □ | e0191133 e0191133
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| ○都市 (任意): |
| ○年月日 (必須): | □ | 西暦 2018年 1月 25日 (平成 30年 1月 25日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1371/journal.pone.0191133 (→Scopusで検索)
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| ○PMID (任意): | □ | 29370185 (→Scopusで検索)
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| ○CRID (任意): |
| ○Scopus (任意): | 1. | 2-s2.0-85041102689
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| ○備考 (任意): | 1. | (英) Article.ELocationID: 10.1371/journal.pone.0191133 (日)
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| 2. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
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| 3. | (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't (日)
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