| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○招待 (推奨): |
| ○審査 (推奨): | □ | Peer Review
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| ○カテゴリ (推奨): | □ | 研究
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| ○共著種別 (推奨): | □ | 学内共著 (徳島大学内研究者との共同研究 (学外研究者を含まない))
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| ○学究種別 (推奨): | □ | 博士課程学生による研究報告
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| ○組織 (推奨): | 1. | 徳島大学.先端酵素学研究所 (2016年4月1日〜)
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| ○著者 (必須): | 1. | (英) Kim Hyeon Soo (日) Kim Hyeon Soo (読) きむ すひょん
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| ○学籍番号 (推奨): | □ | ****
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| 2. | 宍戸 裕二 ([徳島大学.研究支援・産官学連携センター])
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| 3. | (英) Sogabe Hirofumi (日) 曽我部 浩史 (読) そがべ ひろふみ
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| ○貢献度 (任意): |
| ○学籍番号 (推奨): | □ | ****
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| 4. | (英) Rachadech Wanitcha (日) Rachadech Wanitcha (読) らしゃで わにちゃ
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| ○学籍番号 (推奨): | □ | ****
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| 5. | 頼田 和子
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| 6. | 加藤 有介
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| 7. | 福井 清 ([徳島大学])
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| ○題名 (必須): | □ | (英) Age- and gender-dependent D-amino acid oxidase activity in mouse brain and peripheral tissues: implication for aging and neurodegeneration (日)
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| ○副題 (任意): |
| ○要約 (任意): | □ | (英) D-amino acid oxidase (DAO) is a flavoenzyme, catalysing oxidative deamination of D-amino acids to produce corresponding α-keto acids, ammonia and hydrogen peroxide. In our search for DAO activity among various tissues, we developed a sensitive assay based on hydrogen peroxide production involving enzyme-coupled colorimetric assay with peroxidase. We first optimized buffer components to extract DAO protein from mouse tissues. Here we show that DAO activity was detected in kidney, cerebellum, medulla oblongata, midbrain and spinal cord, but not in liver. In addition, we observed that DAO activity and expression were decreased in thoracic and lumbar regions of spinal cord in aged mice when compared with young mice, indicating that decreased DAO is involved in motoneuron degeneration during senescence. We also found gender difference in DAO activity in the kidney, suggesting that DAO activity is influenced by sexual dimorphism. We newly detected DAO activity in the epididymis, although undetected in testis. Furthermore, DAO activity was significantly higher in the caput region than corpus and cauda regions of epididymis, indicating that D-amino acids present in the testis are eliminated in epididymis. Taken together, age- and gender-dependent DAO activity in each organ may underlie the human pathophysiology regulated by D-amino acid metabolism. (日)
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| ○キーワード (推奨): |
| ○発行所 (推奨): | □ | 日本生化学会
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| ○誌名 (必須): | □ | The Journal of Biochemistry ([日本生化学会])
(pISSN: 0021-924X, eISSN: 1756-2651)
| ○ISSN (任意): | □ | 1756-2651
ISSN: 0021-924X
(pISSN: 0021-924X, eISSN: 1756-2651) Title: Journal of biochemistryTitle(ISO): J BiochemSupplier: Oxford University PressPublisher: Oxford University Press (NLM Catalog)
(医中誌Web)
(J-STAGE)
(Scopus)
(CrossRef)
(Scopus information is found. [need login]) | [継承] |
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| ○巻 (必須): | □ | 166
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| ○号 (必須): | □ | 2
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| ○頁 (必須): | □ | 187 196
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| ○都市 (任意): |
| ○年月日 (必須): | □ | 西暦 2019年 8月 1日 (令和 元年 8月 1日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1093/jb/mvz025 (→Scopusで検索)
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| ○PMID (任意): | □ | 30938755 (→Scopusで検索)
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| ○被引用数 (任意): |
| ○指導教員 (推奨): | 1. | 福井 清 ([徳島大学])
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| ○備考 (任意): | 1. | (英) Article.ELocationID: 10.1093/jb/mvz025 (日)
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| 2. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
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| 3. | (英) KeywordList.Keyword: D-amino acid oxidase (日)
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| 4. | (英) KeywordList.Keyword: age- and gender-dependence (日)
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| 5. | (英) KeywordList.Keyword: epididymis (日)
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| 6. | (英) KeywordList.Keyword: kidney (日)
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| 7. | (英) KeywordList.Keyword: spinal cord (日)
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