| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○組織 (推奨): |
| ○著者 (必須): | 1. | (英) Afroz S (日) SHAISTA AFROZ (読) しゃいすた あふろず
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| ○学籍番号 (推奨): | □ | ****
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| 2. | 新垣 理恵子
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| 3. | 岩浅 匠真
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| ○学籍番号 (推奨): | □ | ****
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| 4. | 大島 正充 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.臨床歯学系.顎機能咬合再建学])
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| 5. | 細木 眞紀 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.臨床歯学系.顎機能咬合再建学])
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| 6. | 井上 美穂 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.臨床歯学系.顎機能咬合再建学])
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| 7. | 馬場 麻人 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.基礎歯学系.口腔顎顔面形態学])
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| 8. | (英) Okayama Yoshihiro (日) (読)
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| 9. | 松香 芳三 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.臨床歯学系.顎機能咬合再建学])
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| ○題名 (必須): | □ | (英) CGRP induces differential regulation of cytokines from satellite glial cells in trigeminal ganglia and orofacial nociception (日)
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| ○要約 (任意): | □ | (英) Neuron-glia interactions contribute to pain initiation and sustainment. Intra-ganglionic (IG) secretion of calcitonin gene-related peptide (CGRP) in the trigeminal ganglion (TG) modulates pain transmission through neuron-glia signaling, contributing to various orofacial pain conditions. The present study aimed to investigate the role of satellite glial cells (SGC) in TG in causing cytokine-related orofacial nociception in response to IG administration of CGRP. For that purpose, CGRP alone (10 μL of 10 M), Minocycline (5 μL containing 10 μg) followed by CGRP with one hour gap (Min + CGRP) were administered directly inside the TG in independent experiments. Rats were evaluated for thermal hyperalgesia at 6 and 24 h post-injection using an operant orofacial pain assessment device (OPAD) at three temperatures (37, 45 and 10 °C). Quantitative real-time PCR was performed to evaluate the mRNA expression of IL-1β, IL-6, TNF-α, IL-1 receptor antagonist (IL-1RA), sodium channel 1.7 (NaV 1.7, for assessment of neuronal activation) and glial fibrillary acidic protein (GFAP, a marker of glial activation). The cytokines released in culture media from purified glial cells were evaluated using antibody cytokine array. IG CGRP caused heat hyperalgesia between 6-24 h (paired- test, < 0.05). Between 1 to 6 h the mRNA and protein expressions of GFAP was increased in parallel with an increase in the mRNA expression of pro-inflammatory cytokines IL-1β and anti-inflammatory cytokine IL-1RA and NaV1.7 (one-way ANOVA followed by Dunnett's post hoc test, < 0.05). To investigate whether glial inhibition is useful to prevent nociception symptoms, Minocycline (glial inhibitor) was administered IG 1 h before CGRP injection. Minocycline reversed CGRP-induced thermal nociception, glial activity, and down-regulated IL-1β and IL-6 cytokines significantly at 6 h (-test, < 0.05). Purified glial cells in culture showed an increase in release of 20 cytokines after stimulation with CGRP. Our findings demonstrate that SGCs in the sensory ganglia contribute to the occurrence of pain via cytokine expression and that glial inhibition can effectively control the development of nociception. (日)
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| ○キーワード (推奨): |
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| ○誌名 (必須): | □ | International Journal of Molecular Sciences (Molecular Diversity Preservation International/Multidisciplinary Digital Publishing Institute)
(pISSN: 1661-6596, eISSN: 1422-0067)
| ○ISSN (任意): | □ | 1422-0067
ISSN: 1661-6596
(pISSN: 1661-6596, eISSN: 1422-0067) Title: International journal of molecular sciencesTitle(ISO): Int J Mol SciPublisher: Multidisciplinary Digital Publishing Institute (MDPI) (NLM Catalog)
(Scopus)
(CrossRef)
(Scopus information is found. [need login]) | [継承] |
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| ○巻 (必須): | □ | 20
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| ○号 (必須): | □ | 3
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| ○頁 (必須): | □ | 711 711
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| ○都市 (任意): |
| ○年月日 (必須): | □ | 西暦 2019年 2月 1日 (平成 31年 2月 1日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.3390/ijms20030711 (→Scopusで検索)
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| ○PMID (任意): | □ | 30736422 (→Scopusで検索)
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| ○Scopus (任意): | □ | 2-s2.0-85061270594
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| ○備考 (任意): | 1. | (英) Article.ELocationID: E711 (日)
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| 2. | (英) Article.ELocationID: 10.3390/ijms20030711 (日)
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| 3. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
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| 4. | (英) KeywordList.Keyword: calcitonin gene related peptide (日)
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| 5. | (英) KeywordList.Keyword: cytokine (日)
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| 6. | (英) KeywordList.Keyword: satellite glial cells (日)
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| 7. | (英) KeywordList.Keyword: thermal hyperalgesia (日)
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| 8. | (英) KeywordList.Keyword: trigeminal ganglion (日)
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