| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○招待 (推奨): |
| ○審査 (推奨): |
| ○カテゴリ (推奨): | □ | 研究
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| ○学究種別 (推奨): |
| ○組織 (推奨): |
| ○著者 (必須): | 1. | (英) Satoru Fujimoto (日) (読)
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| 2. | (英) Shigeo S. Sugano (日) (読)
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| 3. | (英) Keiko Kuwata (日) (読)
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| 4. | 刑部 敬史 ([徳島大学.大学院社会産業理工学研究部.生物資源産業学域.生物生産系.生物資源生産科学分野]/[徳島大学.生物資源産業学部.生物資源産業学科.生物生産システムコース])
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| 5. | (英) Sachihiro Matsunaga (日) (読)
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| ○題名 (必須): | □ | (英) Visualization of specific repetitive genomic sequences with fluorescent TALEs in Arabidopsis thaliana. (日)
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| ○要約 (任意): | □ | (英) Live imaging of the dynamics of nuclear organization provides the opportunity to uncover the mechanisms responsible for four-dimensional genome architecture. Here, we describe the use of fluorescent protein (FP) fusions of transcription activator-like effectors (TALEs) to visualize endogenous genomic sequences in Arabidopsis thaliana. The ability to engineer sequence-specific TALEs permits the investigation of precise genomic sequences. We could detect TALE-FP signals associated with centromeric, telomeric, and rDNA repeats and the signal distribution was consistent with that observed by fluorescent in situ hybridization. TALE-FPs are advantageous because they permit the observation of intact tissues. We used our TALE-FP method to investigate the nuclei of several multicellular plant tissues including roots, hypocotyls, leaves, and flowers. Because TALE-FPs permit live-cell imaging, we successfully observed the temporal dynamics of centromeres and telomeres in plant organs. Fusing TALEs to multimeric FPs enhanced the signal intensity when observing telomeres. We found that the mobility of telomeres was different in sub-nuclear regions. Transgenic plants stably expressing TALE-FPs will provide new insights into chromatin organization and dynamics in multicellular organisms. (日)
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| ○キーワード (推奨): | 1. | (英) Arabidopsis (日) (読)
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| 2. | (英) Genome, Plant (日) (読)
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| 3. | (英) Green Fluorescent Proteins (日) (読)
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| 4. | (英) Immunoprecipitation (日) (読)
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| 5. | (英) In Situ Hybridization, Fluorescence (日) (読)
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| 6. | (英) Repetitive Sequences, Nucleic Acid (日) (読)
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| 7. | (英) Transcription Activator-Like Effectors (日) (読)
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| ○発行所 (推奨): |
| ○誌名 (必須): | □ | Journal of Experimental Botany (Society for Experimental Biology (Great Britain)/Federation of European Societies of Plant Physiology)
(pISSN: 0022-0957, eISSN: 1460-2431)
| ○ISSN (任意): | □ | 1460-2431
ISSN: 0022-0957
(pISSN: 0022-0957, eISSN: 1460-2431) Title: Journal of experimental botanyTitle(ISO): J Exp BotSupplier: Oxford University PressPublisher: Oxford University Press (NLM Catalog)
(Scopus)
(CrossRef)
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| ○巻 (必須): | □ | 67
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| ○号 (必須): | □ | 21
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| ○頁 (必須): | □ | 6101 6110
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| ○都市 (任意): |
| ○年月日 (必須): | □ | 西暦 2016年 11月 初日 (平成 28年 11月 初日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1093/jxb/erw371 (→Scopusで検索)
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| ○PMID (任意): | □ | 27811079 (→Scopusで検索)
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| ○Scopus (任意): | 1. | 2-s2.0-84994410891
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| ○備考 (任意): | 1. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
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| 2. | (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't (日)
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| 3. | (英) KeywordList.Keyword: Centromere (日)
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| 4. | (英) KeywordList.Keyword: chromatin (日)
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| 5. | (英) KeywordList.Keyword: fluorescent protein (日)
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| 6. | (英) KeywordList.Keyword: genome editing (日)
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| 7. | (英) KeywordList.Keyword: live cell imaging (日)
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| 8. | (英) KeywordList.Keyword: rDNA (日)
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| 9. | (英) KeywordList.Keyword: telomere (日)
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| 10. | (英) KeywordList.Keyword: transcription activator-like effector. (日)
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