| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○著者 (必須): | 1. | (英) Nishio Katsutoshi (日) 西尾 克俊 (読) にしお かつとし
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| ○学籍番号 (推奨): | □ | ****
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| 2. | (英) Yamazaki Mado (日) (読)
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| 3. | (英) Taniguchi Masayasu (日) (読)
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| 4. | (英) Besshi Kazuhiko (日) (読)
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| 5. | (英) Morita Fumio (日) (読)
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| 6. | (英) Kunihara Toshiki (日) 槶原 俊樹 (読) くにはら としき
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| ○学籍番号 (推奨): | □ | ****
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| 7. | 谷原 史倫 ([徳島大学.バイオイノベーション研究所])
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| 8. | 竹本 龍也 ([徳島大学.先端酵素学研究所.基幹研究部門])
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| 9. | 音井 威重
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| ○題名 (必須): | □ | (英) Sensitivity of the meiotic stage to hyperthermia during in vitro maturation of porcine oocytes. (日)
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| ○要約 (任意): | □ | (英) The present study was conducted to clarify whether the meiotic stage of porcine oocytes has the highest sensitivity to hyperthermia during in vitro maturation by evaluating meiotic competence and DNA damage. Oocytes were exposed to 41 °C for 12 h at various intervals during 48 h of maturation culture. When the oocytes were exposed to 41 °C from 12 to 24 h of the maturation culture, the proportion of oocytes reaching metaphase II (MII) decreased as compared to the control oocytes cultured at 38.5 °C (P < 0.05). Moreover, the proportions of DNA fragmentation in all oocytes exposed to 41 °C in each culture period after 12 h from the start of maturation culture were significantly higher (P < 0.05) than for the control oocytes. When the meiotic stage of oocytes cultured at 38.5 °C between 12 and 24 h was examined, the majority of oocytes remained at the germinal vesicle (GV) stage at 12 h and approximately half of the oocytes reached metaphase I (MI) at 24 h. These results indicate that the meiotic stage of porcine oocytes having the highest sensitivity to hyperthermia during in vitro maturation is a transition period from the GV stage to the MI stage. (日)
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| ○キーワード (推奨): | 1. | (英) Animals (日) (読)
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| 2. | (英) DNA Damage (日) (読)
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| 3. | (英) Hot Temperature (日) (読)
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| 4. | (英) In Vitro Oocyte Maturation Techniques (日) (読)
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| 5. | (英) Meiosis (日) (読)
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| 6. | (英) Oocytes (日) (読)
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| 7. | (英) Swine (日) (読)
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| ○発行所 (推奨): |
| ○誌名 (必須): | □ | Acta Veterinaria Hungarica (Magyar Tudományos Akadémia)
(pISSN: 0236-6290)
| ○ISSN (任意): | □ | 0236-6290
ISSN: 0236-6290
(pISSN: 0236-6290, eISSN: 1588-2705) Title: Acta veterinaria HungaricaTitle(ISO): Acta Vet HungPublisher: Akademiai Kiado ZRt. (NLM Catalog)
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(CrossRef)
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| ○巻 (必須): | □ | 65
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| ○号 (必須): | □ | 1
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| ○頁 (必須): | □ | 115 123
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| ○都市 (任意): |
| ○年月日 (必須): | □ | 西暦 2017年 3月 初日 (平成 29年 3月 初日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1556/004.2017.012 (→Scopusで検索)
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| ○PMID (任意): | □ | 28244334 (→Scopusで検索)
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| ○備考 (任意): | 1. | (英) PublicationType: Journal Article (日)
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| 2. | (英) PublicationType: Research Support, Non-U.S. Gov't (日)
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