『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=343353EID:343353, Map:0, LastModified:2019年5月29日(水) 20:20:25, Operator:[大家 隆弘], Avail:TRUE, Censor:0, Owner:[鬼塚 正義], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
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審査 (推奨):
カテゴリ (推奨):
共著種別 (推奨): 国内共著 (徳島大学内研究者と国内(学外)研究者との共同研究 (国外研究者を含まない)) [継承]
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1.鬼塚 正義 ([徳島大学.大学院社会産業理工学研究部.生物資源産業学域.応用生命系.生体分子機能学分野]/[徳島大学.生物資源産業学部.生物資源産業学科.応用生命講座])
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2. (英) Kinoshita Yukie (日) 木下 幸恵 (読) きのした ゆきえ
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学籍番号 (推奨): **** [ユーザ]
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3. (英) Nishizawa Akitoshi (日) (読)
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4. (英) Tsutsui Tomomi (日) (読)
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5.大政 健史
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題名 (必須): (英) Enhanced IgG1 production by overexpression of nuclear factor kappa B inhibitor zeta (NFKBIZ) in Chinese hamster ovary cells  (日)    [継承]
副題 (任意):
要約 (任意): (英) Several engineering strategies have been employed to improve the production of therapeutic recombinant proteins in Chinese hamster ovary (CHO) cell lines. We have focused on unfolded protein response-based engineering and reported that ATF4 overexpression increases protein production. In this study, transcriptome analysis of ATF4-overexpressed CHO cells was performed using high-coverage expression profiling, to search for another key factor contributing to recombinant protein production. We observed the upregulated expression of transcription factor, nuclear factor (NF)-kappa-B inhibitor zeta (NFKBIZ or Iκbζ), in ATF4-overexpressed cells. A total of 1917 bp of CHO NFKBIZ cDNA was cloned, and two stable cell lines overexpressing NFKBIZ were constructed. We investigated the effects of NFKBIZ on IgG1 production in CHO cells. Although the two stable cell lines, NFKBIZ-A and -B, had the opposite phenotypes in cell growth, the specific IgG1 production rate of both cell lines was enhanced by 1.2-1.4-fold. In the NFKBIZ-A cell line, the synergistic effect between enhanced viable cell density and improved specific IgG1 production rate brought about a large increase in the final IgG1 titer. Luciferase-based NF-κB signaling assay results suggest that altered p50/p50 signaling seems to be due to the opposite phenotypes in cell growth. No difference was observed in the translational levels and intracellular assembly states of IgG1 between mock and two NFKBIZ cell lines, indicating that the secretion machinery of correctly folded IgG1 was enhanced in NFKBIZ-overexpressing cell lines.  (日)    [継承]
キーワード (推奨): 1. (英) High-coverage expression profiling (HiCEP) (日) (読) [継承]
2. (英) NF-kappa B inhibitor zeta (NFKBIZ) (日) (読) [継承]
3. (英) Antibody production (日) (読) [継承]
4. (英) Chinese hamster ovary (CHO) cells (日) (読) [継承]
発行所 (推奨):
誌名 (必須): Cytotechnology (Springer Netherlands)
(pISSN: 0920-9069, eISSN: 1573-0778)

ISSN (任意): 0920-9069
ISSN: 0920-9069 (pISSN: 0920-9069, eISSN: 1573-0778)
Title: Cytotechnology
Title(ISO): Cytotechnology
Supplier: Kluwer Online
Publisher: Springer Netherlands
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 70 [継承]
(必須): 2 [継承]
(必須): 675 685 [継承]
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年月日 (必須): 西暦 2018年 4月 初日 (平成 30年 4月 初日) [継承]
URL (任意):
DOI (任意): 10.1007/s10616-017-0170-8    (→Scopusで検索) [継承]
PMID (任意): 29188404    (→Scopusで検索) [継承]
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Scopus (任意): 2-s2.0-85035361000 [継承]
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備考 (任意): 1.(英) Article.ELocationID: 10.1007/s10616-017-0170-8  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) KeywordList.Keyword: Antibody production  (日)    [継承]
4.(英) KeywordList.Keyword: Chinese hamster ovary (CHO) cells  (日)    [継承]
5.(英) KeywordList.Keyword: High-coverage expression profiling (HiCEP)  (日)    [継承]
6.(英) KeywordList.Keyword: NF-kappa B inhibitor zeta (NFKBIZ)  (日)    [継承]

標準的な表示

和文冊子 ● Masayoshi Onitsuka, Yukie Kinoshita, Akitoshi Nishizawa, Tomomi Tsutsui and Takeshi Omasa : Enhanced IgG1 production by overexpression of nuclear factor kappa B inhibitor zeta (NFKBIZ) in Chinese hamster ovary cells, Cytotechnology, Vol.70, No.2, 675-685, 2018.
欧文冊子 ● Masayoshi Onitsuka, Yukie Kinoshita, Akitoshi Nishizawa, Tomomi Tsutsui and Takeshi Omasa : Enhanced IgG1 production by overexpression of nuclear factor kappa B inhibitor zeta (NFKBIZ) in Chinese hamster ovary cells, Cytotechnology, Vol.70, No.2, 675-685, 2018.

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