| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○カテゴリ (推奨): |
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| ○組織 (推奨): |
| ○著者 (必須): | 1. | 原 英之
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| 2. | (英) Miyata Hironori (日) (読)
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| 3. | (英) Nandita Rani Das (日) Nandita Rani Das (読) なんでぃた らに だす
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| ○学籍番号 (推奨): | □ | ****
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| 4. | 千田 淳司
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| 5. | (英) Yoshimochi Tatenobu (日) 吉持 盾信 (読) よしもち たてのぶ
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| ○学籍番号 (推奨): | □ | ****
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| 6. | 内山 圭司
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| 7. | (英) Watanabe Hitomi (日) (読)
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| 8. | (英) Kondoh Gen (日) (読)
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| 9. | (英) Yokoyama Takashi (日) (読)
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| 10. | 坂口 末廣 ([徳島大学.先端酵素学研究所.基幹研究部門])
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| ○題名 (必須): | □ | (英) Prion Protein Devoid of the Octapeptide Repeat Region Delays BSE Pathogenesis in Mice. (日)
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| ○要約 (任意): | □ | (英) Conformational conversion of the cellular isoform of prion protein, PrP, into the abnormally folded, amyloidogenic isoform, PrP, is a key pathogenic event in prion diseases, including Creutzfeldt-Jakob disease in humans and scrapie and bovine spongiform encephalopathy (BSE) in animals. We previously reported that the octapeptide repeat (OR) region could be dispensable for converting PrP into PrP after infection with RML prions. We demonstrated that mice transgenically expressing mouse PrP with deletion of the OR region on the PrP knockout background, designated Tg(PrPΔOR)/ mice, did not show reduced susceptibility to RML scrapie prions, with abundant accumulation of PrPΔOR in their brains. We show here that Tg(PrPΔOR)/ mice were highly resistant to BSE prions, developing the disease with markedly elongated incubation times after infection with BSE prions. The conversion of PrPΔOR into PrPΔOR was markedly delayed in their brains. These results suggest that the OR region may have a crucial role in the conversion of PrP into PrP after infection with BSE prions. However, Tg(PrPΔOR)/ mice remained susceptible to RML and 22L scrapie prions, developing the disease without elongated incubation times after infection with RML and 22L prions. PrPΔOR accumulated only slightly less in the brains of RML- or 22L-infected Tg(PrPΔOR)/ mice than PrP in control wild-type mice. Taken together, these results indicate that the OR region of PrP could play a differential role in the pathogenesis of BSE prions and RML or 22L scrapie prions. Structure-function relationship studies of PrP conformational conversion into PrP are worthwhile to understand the mechanism of the conversion of PrP into PrP We show here that, by inoculating Tg(PrPΔOR)/ mice with the three different strains of RML, 22L, and BSE prions, the OR region could play a differential role in the conversion of PrP into PrP after infection with RML or 22L scrapie prions and BSE prions. PrPΔOR was efficiently converted into PrPΔOR after infection with RML and 22L prions. However, the conversion of PrPΔOR into PrPΔOR was markedly delayed after infection with BSE prions. Further investigation into the role of the OR region in the conversion of PrP into PrP after infection with BSE prions might be helpful for understanding the pathogenesis of BSE prions. (日)
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| ○キーワード (推奨): | 1. | (英) Animals (日) (読)
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| 2. | 脳 (brain)
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| 3. | (英) Cattle (日) (読)
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| 4. | (英) Disease Susceptibility (日) (読)
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| 5. | (英) Encephalopathy, Bovine Spongiform (日) (読)
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| 6. | (英) Humans (日) (読)
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| 7. | (英) Mice (日) (読)
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| 8. | トランスジェニックマウス (transgenic mice)
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| 9. | (英) Oligopeptides (日) (読)
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| 10. | (英) PrPC Proteins (日) (読)
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| 11. | (英) Prion Diseases (日) (読)
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| 12. | (英) Prions (日) (読)
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| 13. | (英) Sequence Deletion (日) (読)
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| ○発行所 (推奨): |
| ○誌名 (必須): | □ | Journal of Virology ([アメリカ微生物学会])
(pISSN: 0022-538X, eISSN: 1098-5514)
| ○ISSN (任意): | □ | 1098-5514
ISSN: 0022-538X
(pISSN: 0022-538X, eISSN: 1098-5514) Title: Journal of virologyTitle(ISO): J VirolPublisher: American Society for Microbiology (NLM Catalog)
(Scopus)
(CrossRef)
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| ○巻 (必須): | □ | 92
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| ○号 (必須): | □ | 1
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| ○頁 (必須): | □ | pii:e01368-17 pii:e01368-17
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| ○都市 (任意): |
| ○年月日 (必須): | □ | 西暦 2018年 0月 初日 (平成 30年 0月 初日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1128/JVI.01368-17 (→Scopusで検索)
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| ○PMID (任意): | □ | 29046443 (→Scopusで検索)
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| ○CRID (任意): | □ | 1050867133852477440
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| ○機関リポジトリ : | □ | 111465
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| ○備考 (任意): | 1. | (英) Article.ELocationID: e01368-17 (日)
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| 2. | (英) Article.ELocationID: 10.1128/JVI.01368-17 (日)
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| 3. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
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| 4. | (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't (日)
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| 5. | (英) KeywordList.Keyword: BSE (日)
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| 6. | (英) KeywordList.Keyword: bovine spongiform encephalopathy (日)
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| 7. | (英) KeywordList.Keyword: octapeptide repeat (日)
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| 8. | (英) KeywordList.Keyword: prion (日)
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| 9. | (英) KeywordList.Keyword: prion protein (日)
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| 10. | (英) KeywordList.Keyword: scrapie (日)
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