『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=332554EID:332554, Map:0, LastModified:2017年8月29日(火) 13:36:32, Operator:[大家 隆弘], Avail:TRUE, Censor:0, Owner:[堤 理恵], Read:継承, Write:継承, Delete:継承.
種別 (必須):
言語 (必須): 英語 [継承]
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著者 (必須): 1. (英) Shetty Abhishek (日) (読)
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2. (英) Venkatesh Thejaswini (日) (読)
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3. (英) Suresh S. Padmanaban (日) (読)
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4.堤 理恵 ([徳島大学.大学院医歯薬学研究部.医学域.栄養科学部門.医科栄養学系.代謝栄養学])
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題名 (必須): (英) Exploration of acute genotoxic effects and antigenotoxic potential of gambogic acid using Allium cepa assay.  (日)    [継承]
副題 (任意):
要約 (任意): (英) The plant derived xanthanoid gambogic acid (GA) is well known for its anticancer activity. To date, biological actions of GA on plant system have not been reported. In the present study, we evaluated the potential acute genotoxic activity of GA, and its antigenotoxic potential against H2O2 induced genetic damage using Allium cepa root chromosomal aberration assay under hydroponic conditions. There was a significant decrease in the percentage of mitotic index/prophase index with the increase in clastogenicity percentage in a dose and time-dependent manner when Allium cepa bulbs were exposed to GA at 0.1 mM and 1 mM concentration for 1 h, 2 h, and 4 h. Total genomic DNA integrity analyzed by agarose gel electrophoresis and cell viability revealed pronounced DNA degradation and loss of viability when treated with 1 mM GA for 4 h. In situ histochemical localization by Schiff's staining and 3, 3-diaminobenzidine confirmed increased levels of lipid peroxide and H2O2 in GA treated roots respectively. Scanning electron microscopy and FT-IR suggested surface damage and biomolecular intervention of GA in root cells. In addition, possible antigenotoxic effect of GA at lower concentration was explored by employing standard assays using H2O2. We observed a higher percentage of nuclear lesions upon treatment with 3% H2O2 (97.21 ± 0.76) that reduced significantly after modulatory treatment with 0.01 mM GA (70.44 ± 4.42). The results suggest that GA is a Janus-faced compound as it demonstrates a genotoxic activity at higher doses and genoprotective action at lower precise doses.  (日)    [継承]
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誌名 (必須): Plant Physiology and Biochemistry : PPB (Société française de physiologie végétale/Federation of European Societies of Plant Physiology)
(pISSN: 0981-9428, eISSN: 1873-2690)

ISSN (任意): 1873-2690
ISSN: 0981-9428 (pISSN: 0981-9428, eISSN: 1873-2690)
Title: Plant physiology and biochemistry : PPB
Title(ISO): Plant Physiol Biochem
Publisher: Elsevier BV
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 118 [継承]
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(必須): 643 652 [継承]
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年月日 (必須): 西暦 2017年 8月 5日 (平成 29年 8月 5日) [継承]
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DOI (任意): 10.1016/j.plaphy.2017.08.005    (→Scopusで検索) [継承]
PMID (任意): 28806720    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) Article.ELocationID: S0981-9428(17)30256-5  (日)    [継承]
2.(英) Article.ELocationID: 10.1016/j.plaphy.2017.08.005  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
4.(英) KeywordList.Keyword: Clastogenic  (日)    [継承]
5.(英) KeywordList.Keyword: Gambogic acid  (日)    [継承]
6.(英) KeywordList.Keyword: Genotoxic  (日)    [継承]
7.(英) KeywordList.Keyword: Root tip  (日)    [継承]

標準的な表示

和文冊子 ● (種別) : Abhishek Shetty, Thejaswini Venkatesh, Padmanaban S. Suresh and Rie Tsutsumi : Exploration of acute genotoxic effects and antigenotoxic potential of gambogic acid using Allium cepa assay., Plant Physiology and Biochemistry : PPB, Vol.118, (号), 643-652, (発行所), (都市), Aug. 2017.
欧文冊子 ● (種別) : Abhishek Shetty, Thejaswini Venkatesh, Padmanaban S. Suresh and Rie Tsutsumi : Exploration of acute genotoxic effects and antigenotoxic potential of gambogic acid using Allium cepa assay., Plant Physiology and Biochemistry : PPB, Vol.118, (号), 643-652, (発行所), (都市), Aug. 2017.

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