| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○組織 (推奨): |
| ○著者 (必須): | 1. | 宮脇 慎吾
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| 2. | (英) Okada yohei (日) (読)
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| 3. | (英) Okano Hideyuki (日) (読)
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| 4. | (英) Miura Kyoko (日) (読)
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| ○題名 (必須): | □ | (英) Teratoma Formation Assay for Assessing Pluripotency and Tumorigenicity of Pluripotent Stem Cells (日)
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| ○要約 (任意): | □ | (英) Pluripotent stem cells such as induced pluripotent stem cells (iPSCs) and embryonic stem cells (ESCs) form teratomas when transplanted into immunodeficient mice. As teratomas contain all three germ layers (endoderm, mesoderm, ectoderm), teratoma formation assay is widely used as an index of pluripotency (Evans and Kaufman, 1981; Hentze , 2009 ; Gropp , 2012 ). On the other hand, teratoma-forming tumorigenicity also represents a major risk factor impeding potential clinical applications of pluripotent stem cells ( Miura , 2009 ; Okano , 2013 ). Recently, we reported that iPSCs derived from naked mole-rat lack teratoma-forming tumorigenicity when engrafted into the testes of non-obese diabetic/severe combined immunodeficient (NOD/SCID) mice due to an ES cell-expressed Ras (ERAS) and Alternative reading frame (ARF)-dependent tumor-suppression mechanism specific to this species ( Miyawaki , 2016 ). Here, we describe a method for transplanting pluripotent stem cells into the testes of NOD/SCID mice to generate teratomas for assessing the pluripotency and tumorigenicity. (日)
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| ○キーワード (推奨): |
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| ○誌名 (必須): | □ | Bio-protocol (Bio-protocol LLC)
(eISSN: 2331-8325)
| ○ISSN (任意): | □ | 2331-8325
ISSN: 2331-8325
(eISSN: 2331-8325) Title: Bio-protocolTitle(ISO): Bio ProtocPublisher: Bio-protocol LLC (NLM Catalog)
(Scopus)
(CrossRef)
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| ○巻 (必須): | □ | 7
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| ○号 (必須): | □ | 16
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| ○頁 (必須): | □ | e2518 e2518
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| ○都市 (任意): |
| ○年月日 (必須): | □ | 西暦 2017年 8月 20日 (平成 29年 8月 20日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.21769/BioProtoc.2518 (→Scopusで検索)
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| ○PMID (任意): | □ | 34541178 (→Scopusで検索)
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| ○備考 (任意): | 1. | (英) Article.ELocationID: e2518 (日)
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| 2. | (英) Article.ELocationID: 10.21769/BioProtoc.2518 (日)
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| 3. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
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| 4. | (英) KeywordList.Keyword: NOD/SCID mice (日)
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| 5. | (英) KeywordList.Keyword: Pluripotent stem cells (日)
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| 6. | (英) KeywordList.Keyword: Teratoma (日)
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| 7. | (英) KeywordList.Keyword: Testis (日)
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| 8. | (英) KeywordList.Keyword: Tumorigenicity (日)
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