| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○招待 (推奨): |
| ○審査 (推奨): |
| ○カテゴリ (推奨): |
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| ○学究種別 (推奨): |
| ○組織 (推奨): |
| ○著者 (必須): | 1. | (英) Kamada Rui (日) (読)
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| 2. | (英) Tano Fumi (日) (読)
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| 3. | (英) Kudoh Fuki (日) (読)
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| 4. | (英) Kimura Nozomi (日) (読)
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| 5. | (英) Chuman Yoshiro (日) (読)
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| 6. | (英) Osawa Ayumi (日) (読)
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| 7. | 難波 康祐 ([徳島大学.大学院医歯薬学研究部.薬学域.薬科学部門.総合薬学教育学系.総合薬学研究推進学])
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| 8. | (英) Tanino Keiji (日) (読)
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| 9. | (英) Sakaguchi Kazuyasu (日) (読)
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| ○題名 (必須): | □ | (英) Effective Cellular Morphology Analysis for Differentiation Processes by a Fluorescent 1,3a,6a-Triazapentalene Derivative Probe in Live Cells (日)
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| ○副題 (任意): |
| ○要約 (任意): | □ | (英) Nuclear and cytoplasmic morphological changes provide important information about cell differentiation processes, cell functions, and signal responses. There is a strong desire to develop a rapid and simple method for visualizing cytoplasmic and nuclear morphology. Here, we developed a novel and rapid method for probing cellular morphological changes of live cell differentiation process by a fluorescent probe, TAP-4PH, a 1,3a,6a-triazapentalene derivative. TAP-4PH showed high fluorescence in cytoplasmic area, and visualized cytoplasmic and nuclear morphological changes of live cells during differentiation. We demonstrated that TAP-4PH visualized dendritic axon and spine formation in neuronal differentiation, and nuclear structural changes during neutrophilic differentiation. We also showed that the utility of TAP-4PH for visualization of cytoplasmic and nuclear morphologies of various type of live cells. Our visualizing method has no toxicity and no influence on the cellular differentiation and function. The cell morphology can be rapidly observed after addition of TAP-4PH and can continue to be observed in the presence of TAP-4PH in cell culture medium. Moreover, TAP-4PH can be easily removed after observation by washing for subsequent biological assay. Taken together, these results demonstrate that our visualization method is a powerful tool to probe differentiation processes before subsequent biological assay in live cells. (日)
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| ○キーワード (推奨): | 1. | (英) 3T3-L1 Cells (日) (読)
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| 2. | (英) A549 Cells (日) (読)
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| 3. | (英) Animals (日) (読)
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| 4. | (英) Bridged Bicyclo Compounds, Heterocyclic (日) (読)
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| 5. | (英) Cell Cycle (日) (読)
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| 6. | (英) Cell Differentiation (日) (読)
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| 7. | (英) Cell Line, Tumor (日) (読)
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| 8. | (英) Cell Nucleus (日) (読)
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| 9. | (英) Cytoplasm (日) (読)
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| 10. | (英) Fluorescent Dyes (日) (読)
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| 11. | (英) HEK293 Cells (日) (読)
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| 12. | (英) Humans (日) (読)
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| 13. | (英) Mice (日) (読)
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| 14. | (英) Microscopy, Fluorescence (日) (読)
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| 15. | (英) PC12 Cells (日) (読)
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| 16. | (英) Phagocytosis (日) (読)
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| 17. | (英) Rats (日) (読)
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| 18. | (英) Spectrometry, Fluorescence (日) (読)
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| ○発行所 (推奨): |
| ○誌名 (必須): | □ | PLoS ONE (Public Library of Science)
(eISSN: 1932-6203)
| ○ISSN (任意): | □ | 1932-6203
ISSN: 1932-6203
(eISSN: 1932-6203) Title: PloS oneTitle(ISO): PLoS OnePublisher: PLOS (NLM Catalog)
(Scopus)
(CrossRef)
(Scopus information is found. [need login]) | [継承] |
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| ○巻 (必須): | □ | 11
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| ○号 (必須): | □ | 8
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| ○頁 (必須): | □ | e0160625 e0160625
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| ○都市 (任意): |
| ○年月日 (必須): | □ | 西暦 2016年 8月 4日 (平成 28年 8月 4日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1371/journal.pone.0160625 (→Scopusで検索)
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| ○PMID (任意): | □ | 27490470 (→Scopusで検索)
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| ○機関リポジトリ : | □ | 112331
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| ○備考 (任意): | 1. | (英) Article.ELocationID: 10.1371/journal.pone.0160625 (日)
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| 2. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
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| 3. | (英) OtherID: PMC4973928 (日)
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