| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○招待 (推奨): |
| ○審査 (推奨): | □ | Peer Review
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| ○カテゴリ (推奨): | □ | 研究
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| ○共著種別 (推奨): | □ | 国内共著 (徳島大学内研究者と国内(学外)研究者との共同研究 (国外研究者を含まない))
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| ○学究種別 (推奨): |
| ○組織 (推奨): |
| ○著者 (必須): | 1. | 堀尾 修平
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| ○題名 (必須): | □ | (英) Distribution of corticotropin-releasing factor neurons in the mouse brain: a study using corticotropin-releasing factor-modified yellow fluorescent protein knock-in mouse. (日)
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| ○要約 (任意): | □ | (英) We examined the morphological features of corticotropin-releasing factor (CRF) neurons in a mouse line in which modified yellow fluorescent protein (Venus) was expressed under the CRF promoter. We previously generated the CRF-Venus knock-in mouse, in which Venus is inserted into the CRF gene locus by homologous recombination. In the present study, the neomycin phosphotransferase gene (Neo), driven by the pgk-1 promoter, was deleted from the CRF-Venus mouse genome, and a CRF-Venus∆Neo mouse was generated. Venus expression is much more prominent in the CRF-Venus∆Neo mouse when compared to the CRF-Venus mouse. In addition, most Venus-expressing neurons co-express CRF mRNA. Venus-expressing neurons constitute a discrete population of neuroendocrine neurons in the paraventricular nucleus of the hypothalamus (PVH) that project to the median eminence. Venus-expressing neurons were also found in brain regions outside the neuroendocrine PVH, including the olfactory bulb, the piriform cortex (Pir), the extended amygdala, the hippocampus, the neocortices, Barrington's nucleus, the midbrain/pontine dorsal tegmentum, the periaqueductal gray, and the inferior olivary nucleus (IO). Venus-expressing perikarya co-expressing CRF mRNA could be observed clearly even in regions where CRF-immunoreactive perikarya could hardly be identified. We demonstrated that the CRF neurons contain glutamate in the Pir and IO, while they contain gamma-aminobutyric acid in the neocortex, the bed nucleus of the stria terminalis, the hippocampus, and the amygdala. A population of CRF neurons was demonstrated to be cholinergic in the midbrain tegmentum. The CRF-Venus∆Neo mouse may be useful for studying the structural and functional properties of CRF neurons in the mouse brain. (日)
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| ○キーワード (推奨): |
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| ○誌名 (必須): | □ | Brain Structure & Function (Springer)
(pISSN: 1863-2653, eISSN: 1863-2661)
| ○ISSN (任意): | □ | 1863-2661
ISSN: 1863-2653
(pISSN: 1863-2653, eISSN: 1863-2661) Title: Brain structure & functionTitle(ISO): Brain Struct FunctPublisher: Springer (NLM Catalog)
(Scopus)
(CrossRef)
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| ○巻 (必須): | □ | 222
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| ○号 (必須): | □ | 4
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| ○頁 (必須): | □ | 1705 1732
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| ○都市 (任意): |
| ○年月日 (必須): | □ | 西暦 2016年 9月 16日 (平成 28年 9月 16日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1007/s00429-016-1303-0 (→Scopusで検索)
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| ○PMID (任意): | □ | 27638512 (→Scopusで検索)
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| ○Scopus (任意): | 1. | 2-s2.0-84988373916
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| ○備考 (任意): | 1. | (英) Article.ELocationID: 10.1007/s00429-016-1303-0 (日)
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| 2. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
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| 3. | (英) KeywordList.Keyword: Glucocorticoids (日)
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| 4. | (英) KeywordList.Keyword: Homologous recombination (日)
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| 5. | (英) KeywordList.Keyword: Immunofluorescence (日)
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| 6. | (英) KeywordList.Keyword: In situ hybridization (日)
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| 7. | (英) KeywordList.Keyword: Stress (日)
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