| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○著者 (必須): | 1. | 亀村 典生
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| 2. | (英) Murakami Sara (日) 村上 彩良 (読) むらかみ さら
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| ○学籍番号 (推奨): | □ | ****
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| 3. | (英) Komatsu Hiroaki (日) 小松 弘明 (読) こまつ ひろあき
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| ○学籍番号 (推奨): | □ | ****
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| 4. | (英) Sawanoi Masahiro (日) 澤野井 政宏 (読) さわのい まさひろ
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| ○学籍番号 (推奨): | □ | ****
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| 5. | (英) Miyamoto Kenji (日) 宮本 賢治 (読) みやもと けんじ
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| ○学籍番号 (推奨): | □ | ****
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| 6. | (英) Ishidoh Kazumi (日) (読)
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| 7. | 岸本 幸治 ([徳島大学.大学院社会産業理工学研究部.生物資源産業学域.食料科学系.食料科学分野]/[徳島大学.生物資源産業学部.生物資源産業学科.応用生命コース]/[徳島大学.大学院社会産業理工学研究部.生物資源産業学域.応用生命系.応用生物資源学分野]/大阪大学蛋白研究所 共同研究員)
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| 8. | 辻 明彦
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| 9. | 湯浅 恵造
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| ○題名 (必須): | □ | (英) Type II cGMP-dependent protein kinase negatively regulates fibroblast growth factor signaling by phosphorylating Raf-1 at serine 43 in rat chondrosarcoma cells. (日)
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| ○要約 (任意): | □ | (英) Although type II cGMP-dependent protein kinase (PKGII) is a major downstream effector of cGMP in chondrocytes and attenuates the FGF receptor 3/ERK signaling pathway, its direct target proteins have not been fully explored. In the present study, we attempted to identify PKGII-targeted proteins, which are associated with the inhibition of FGF-induced MAPK activation. Although FGF2 stimulation induced the phosphorylation of ERK1/2, MEK1/2, and Raf-1 at Ser-338 in rat chondrosarcoma cells, pretreatment with a cell-permeable cGMP analog strongly inhibited their phosphorylation. On the other hand, Ser-43 of Raf-1 was phosphorylated by cGMP in a dose-dependent manner. Therefore, we examined the direct phosphorylation of Raf-1 by PKGII. Wild-type PKGII phosphorylated Raf-1 at Ser-43 in a cGMP-dependent manner, but a PKGII D412A/R415A mutant, which has a low affinity for cGMP, did not. Finally, we found that a phospho-mimic mutant, Raf-1 S43D, suppressed FGF2-induced MAPK pathway. These results suggest that PKGII counters FGF-induced MEK/ERK activation through the phosphorylation of Raf-1 at Ser-43 in chondrocytes. (日)
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| ○誌名 (必須): | □ | Biochemical and Biophysical Research Communications ([Elsevier])
(pISSN: 0006-291X, eISSN: 1090-2104)
| ○ISSN (任意): | □ | 1090-2104
ISSN: 0006-291X
(pISSN: 0006-291X, eISSN: 1090-2104) Title: Biochemical and biophysical research communicationsTitle(ISO): Biochem Biophys Res CommunPublisher: Elsevier B.V. (NLM Catalog)
(Scopus)
(CrossRef)
(Scopus information is found. [need login]) | [継承] |
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| ○巻 (必須): | □ | 483
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| ○号 (必須): | □ | 1
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| ○頁 (必須): | □ | 82 87
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| ○都市 (任意): |
| ○年月日 (必須): | □ | 西暦 2017年 1月 29日 (平成 29年 1月 29日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1016/j.bbrc.2017.01.001 (→Scopusで検索)
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| ○PMID (任意): | □ | 28057484 (→Scopusで検索)
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| ○WOS : | □ | 000397259000013
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| ○Scopus (任意): | 1. | 2-s2.0-85008689720
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| ○機関リポジトリ : | □ | 111887
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| ○備考 (任意): | 1. | (英) Article.ELocationID: S0006-291X(17)30001-3 (日)
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| 2. | (英) Article.ELocationID: 10.1016/j.bbrc.2017.01.001 (日)
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| 3. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
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| 4. | (英) KeywordList.Keyword: Chondrocytes (日)
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| 5. | (英) KeywordList.Keyword: FGF (日)
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| 6. | (英) KeywordList.Keyword: PKGII (日)
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| 7. | (英) KeywordList.Keyword: Raf-1 (日)
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