| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○招待 (推奨): |
| ○審査 (推奨): |
| ○カテゴリ (推奨): |
| ○共著種別 (推奨): | □ | 国内共著 (徳島大学内研究者と国内(学外)研究者との共同研究 (国外研究者を含まない))
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| ○学究種別 (推奨): |
| ○組織 (推奨): |
| ○著者 (必須): | 1. | (英) Murai Masatoshi (日) (読)
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| 2. | (英) Okuda Ayaka (日) (読)
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| 3. | 山本 武範
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| 4. | 篠原 康雄 ([徳島大学.先端酵素学研究所.基幹研究部門])
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| 5. | (英) Miyoshi Hideto (日) (読)
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| ○題名 (必須): | □ | (英) Synthetic Ubiquinones Specifically Bind to Mitochondrial Voltage-Dependent Anion Channel 1 (VDAC1) in Saccharomyces cerevisiae Mitochondria. (日)
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| ○要約 (任意): | □ | (英) The role of voltage-dependent anion channel (VDAC) as a metabolic gate of the mitochondrial outer membrane has been firmly established; however, its involvement in the regulation of mitochondrial permeability transition (PT) remains extremely controversial. Although some low-molecular-weight chemicals have been proposed to modulate the regulatory role of VDAC in the induction of PT, direct binding between these chemicals and VDAC has not yet been demonstrated. In the present study, we investigated whether the ubiquinone molecule directly binds to VDAC in Saccharomyces cerevisiae mitochondria through a photoaffinity labeling technique using two photoreactive ubiquinones (PUQ-1 and PUQ-2). The results of the labeling experiments demonstrated that PUQ-1 and PUQ-2 specifically bind to VDAC1 and that the labeled position is located in the C-terminal region Phe221-Lys234, connecting the 15th and 16th -strand sheets. Mutations introduced in this region (R224A, Y225A, D228A, and Y225A/D228A) hardly affected the binding affinity of PUQ-1. PUQ-1 and PUQ-2 both significantly suppressed the Ca(2+)-induced mitochondrial PT (monitored by mitochondrial swelling) at the one digit µM level. Thus, the results of the present study provided, for the first time to our knowledge, direct evidence indicating that the ubiquinone molecule specifically binds to VDAC1 through its quinone-head ring. (日)
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| ○キーワード (推奨): |
| ○発行所 (推奨): |
| ○誌名 (必須): | □ | Biochemistry ([アメリカ化学会])
(pISSN: 0006-2960, eISSN: 1520-4995)
| ○ISSN (任意): | □ | 1520-4995
ISSN: 0006-2960
(pISSN: 0006-2960, eISSN: 1520-4995) Title: BiochemistryTitle(ISO): BiochemistryPublisher: American Chemical Society (NLM Catalog)
(Scopus)
(CrossRef)
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| ○巻 (必須): | □ | 56
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| ○号 (必須): | □ | 4
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| ○頁 (必須): | □ | 570 581
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| ○年月日 (必須): | □ | 西暦 2017年 1月 4日 (平成 29年 1月 4日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1021/acs.biochem.6b01011 (→Scopusで検索)
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| ○PMID (任意): | □ | 28051849 (→Scopusで検索)
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| ○CRID (任意): |
| ○Scopus (任意): | 1. | 2-s2.0-85011005509
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| ○備考 (任意): | 1. | (英) PublicationType: Journal Article (日)
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