| ○種別 (必須): | □ | 学術論文 (審査論文)
| [継承] |
| ○言語 (必須): | □ | 英語
| [継承] |
| ○招待 (推奨): |
| ○審査 (推奨): |
| ○カテゴリ (推奨): |
| ○共著種別 (推奨): | □ | 国内共著 (徳島大学内研究者と国内(学外)研究者との共同研究 (国外研究者を含まない))
| [継承] |
| ○学究種別 (推奨): |
| ○組織 (推奨): |
| ○著者 (必須): | 1. | 山本 圭 ([徳島大学.大学院社会産業理工学研究部.生物資源産業学域.応用生命系.応用生命科学分野]/[徳島大学.生物資源産業学部.生物資源産業学科.応用生命コース])
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| 2. | 三木 寿美 ([徳島大学.大学院医歯薬学研究部.薬学域.薬科学部門.創薬科学系.創薬理論化学]/[徳島大学.フォトニクス健康フロンティア研究院])
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| 3. | (英) Sato, H. (日) (読)
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| 4. | (英) Murase, R. (日) (読)
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| 5. | (英) Taketomi, Y. (日) (読)
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| 6. | (英) Murakami, M. (日) (読)
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| ○題名 (必須): | □ | (英) Secreted Phospholipase A2 Specificity on Natural Membrane Phospholipids (日)
| [継承] |
| ○副題 (任意): |
| ○要約 (任意): | □ | (英) The secreted phospholipase A2 (sPLA2) family contains 10 catalytically active isoforms. Current in vitro biochemical studies have shown that individual sPLA2s have distinct substrate selectivity in terms of the polar head groups or sn-2 fatty acids of their substrate phospholipids. Importantly, transgenic or knockout mice for distinct sPLA2s display nonoverlapping phenotypes, arguing that they do act on different phospholipid substrates and mobilize unique lipid metabolites in vivo. In an effort to comprehensively understand lipid metabolism driven by individual sPLA2s under pathophysiological conditions, we took advantages of mass spectrometric lipidomics technology to monitor the spatiotemporal changes in phospholipids (substrates) and products (fatty acids, lysophospholipids, and their metabolites) in tissues or cells of sPLA2-transgenic or knockout mice. The in vivo lipidomic data were compared with the in vitro activity of recombinant sPLA2s toward phospholipid mixtures extracted from the target tissues, cells, or extracellular membrane components on which sPLA2s may intrinsically act. These approaches reveal that the overall tendency in in vitro assays using natural membranes is recapitulated in several in vivo systems, often with even more selective patterns of hydrolysis. In this chapter, we will summarize current understanding of the in vivo substrate specificity of sPLA2s toward natural membrane phospholipids. (日)
| [継承] |
| ○キーワード (推奨): |
| ○発行所 (推奨): |
| ○誌名 (必須): | □ | Methods in Enzymology ([Academic Press])
(pISSN: 0076-6879, eISSN: 1557-7988)
| ○ISSN (任意): | □ | 1557-7988
ISSN: 0076-6879
(pISSN: 0076-6879, eISSN: 1557-7988) Title: Methods in enzymologyTitle(ISO): Methods EnzymolPublisher: Academic Press Inc. (NLM Catalog)
(Scopus)
(Scopus information is found. [need login]) | [継承] |
| [継承] |
| ○巻 (必須): | □ | 583
| [継承] |
| ○号 (必須): | □ |
| [継承] |
| ○頁 (必須): | □ | 101 117
| [継承] |
| ○都市 (任意): |
| ○年月日 (必須): | □ | 西暦 2017年 0月 初日 (平成 29年 0月 初日)
| [継承] |
| ○URL (任意): |
| ○DOI (任意): | □ | 10.1016/bs.mie.2016.09.007 (→Scopusで検索)
| [継承] |
| ○PMID (任意): | □ | 28063487 (→Scopusで検索)
| [継承] |
| ○CRID (任意): |
| ○Scopus (任意): | 1. | 2-s2.0-85006993831
| [継承] |
| ○researchmap (任意): |
| ○評価値 (任意): |
| ○被引用数 (任意): |
| ○指導教員 (推奨): |
| ○備考 (任意): | 1. | (英) Article.ELocationID: S0076-6879(16)30280-4 (日)
| [継承] |
| 2. | (英) Article.ELocationID: 10.1016/bs.mie.2016.09.007 (日)
| [継承] |
| 3. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
| [継承] |
| 4. | (英) KeywordList.Keyword: Arachidonic acid (日)
| [継承] |
| 5. | (英) KeywordList.Keyword: Docosahexaenoic acid (日)
| [継承] |
| 6. | (英) KeywordList.Keyword: Knockout mouse (日)
| [継承] |
| 7. | (英) KeywordList.Keyword: Lipidomics (日)
| [継承] |
| 8. | (英) KeywordList.Keyword: Lysophospholipid (日)
| [継承] |
| 9. | (英) KeywordList.Keyword: Mass spectrometry (日)
| [継承] |
| 10. | (英) KeywordList.Keyword: Phospholipid (日)
| [継承] |
| 11. | (英) KeywordList.Keyword: Polyunsaturated fatty acid (日)
| [継承] |
| 12. | (英) KeywordList.Keyword: Secreted phospholipase A(2) (日)
| [継承] |
| 13. | (英) KeywordList.Keyword: Transgenic mouse (日)
| [継承] |