| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○招待 (推奨): |
| ○審査 (推奨): |
| ○カテゴリ (推奨): | □ | 研究
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| ○共著種別 (推奨): | □ | 国内共著 (徳島大学内研究者と国内(学外)研究者との共同研究 (国外研究者を含まない))
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| ○学究種別 (推奨): |
| ○組織 (推奨): |
| ○著者 (必須): | 1. | (英) Nomura, T. (日) (読)
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| 2. | (英) Sakurai, T. (日) (読)
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| 3. | 刑部 祐里子
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| 4. | 刑部 敬史 ([徳島大学.大学院社会産業理工学研究部.生物資源産業学域.生物生産系.生物資源生産科学分野]/[徳島大学.生物資源産業学部.生物資源産業学科.生物生産システムコース])
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| 5. | (英) Sakakibara, H. (日) (読)
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| ○題名 (必須): | □ | (英) Efficient and heritable targeted mutagenesis in mosses using the CRISPR/Cas9 system (日)
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| ○要約 (任意): | □ | (英) Targeted genome modification by RNA-guided nucleases derived from the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated nuclease 9 (Cas9) system has seen rapid development in many organisms, including several plant species. In the present study, we succeeded in introducing the CRISPR/Cas9 system into the non-model organism Scopelophila cataractae, a moss that exhibits heavy metal tolerance, and the model organism Physcomitrella patens Utilizing the process by which moss plants regenerate from protoplasts, we conducted targeted mutagenesis by expression of single-chain guide RNA (sgRNA) and Cas9 in protoplasts. Using this method, the acquisition rate of strains exhibiting phenotypic changes associated with the target genes was approximately 45-69%, and strains with phenotypic changes exhibited various insertion and deletion mutations. In addition, we report that our method is capable of multiplex targeted mutagenesis (two independent genes) and also permits the efficient introduction of large deletions (∼3 kbp). These results demonstrate that the CRISPR/Cas9 system can be used to accelerate investigations of bryology and land plant evolution. (日)
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| ○キーワード (推奨): |
| ○発行所 (推奨): |
| ○誌名 (必須): | □ | Plant & Cell Physiology (日本植物生理学会)
(pISSN: 0032-0781, eISSN: 1471-9053)
| ○ISSN (任意): | □ | 1471-9053
ISSN: 0032-0781
(pISSN: 0032-0781, eISSN: 1471-9053) Title: Plant & cell physiologyTitle(ISO): Plant Cell PhysiolSupplier: 一般社団法人日本植物生理学会Publisher: Oxford University Press (NLM Catalog)
(CiNii NCID)
(Scopus)
(CrossRef)
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| ○巻 (必須): | □ | 57
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| ○号 (必須): | □ | 12
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| ○頁 (必須): | □ | 2600 2610
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| ○都市 (任意): |
| ○年月日 (必須): | □ | 西暦 2016年 10月 22日 (平成 28年 10月 22日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1093/pcp/pcw173 (→Scopusで検索)
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| ○PMID (任意): | □ | 27986915 (→Scopusで検索)
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| ○CRID (任意): |
| ○Scopus (任意): | 1. | 2-s2.0-85014254141
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| ○備考 (任意): | 1. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
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| 2. | (英) KeywordList.Keyword: Bryophyte (日)
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| 3. | (英) KeywordList.Keyword: CRISPR/Cas9 system (日)
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| 4. | (英) KeywordList.Keyword: Genome editing (日)
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| 5. | (英) KeywordList.Keyword: Physcomitrella patens (日)
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| 6. | (英) KeywordList.Keyword: RNA-guided nucleases (日)
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| 7. | (英) KeywordList.Keyword: Scopelophila cataractae (日)
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