| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○審査 (推奨): |
| ○カテゴリ (推奨): |
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| ○学究種別 (推奨): |
| ○組織 (推奨): |
| ○著者 (必須): | 1. | (英) Nishitani Chikako (日) (読)
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| 2. | (英) Hirai Narumi (日) (読)
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| 3. | (英) Komori Sadao (日) (読)
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| 4. | (英) Wada Masato (日) (読)
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| 5. | (英) Okada Kazuma (日) (読)
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| 6. | 刑部 敬史 ([徳島大学.大学院社会産業理工学研究部.生物資源産業学域.生物生産系.生物資源生産科学分野]/[徳島大学.生物資源産業学部.生物資源産業学科.生物生産システムコース])
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| 7. | (英) Yamamoto Toshiya (日) (読)
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| 8. | 刑部 祐里子
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| ○題名 (必須): | □ | (英) Efficient Genome Editing in Apple Using a CRISPR/Cas9 system. (日)
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| ○要約 (任意): | □ | (英) Genome editing is a powerful technique for genome modification in molecular research and crop breeding, and has the great advantage of imparting novel desired traits to genetic resources. However, the genome editing of fruit tree plantlets remains to be established. In this study, we describe induction of a targeted gene mutation in the endogenous apple phytoene desaturase (PDS) gene using the CRISPR/Cas9 system. Four guide RNAs (gRNAs) were designed and stably transformed with Cas9 separately in apple. Clear and partial albino phenotypes were observed in 31.8% of regenerated plantlets for one gRNA, and bi-allelic mutations in apple PDS were confirmed by DNA sequencing. In addition, an 18-bp gRNA also induced a targeted mutation. These CRIPSR/Cas9 induced-mutations in the apple genome suggest activation of the NHEJ pathway, but with some involvement also of the HR pathway. Our results demonstrate that genome editing can be practically applied to modify the apple genome. (日)
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| ○キーワード (推奨): |
| ○発行所 (推奨): |
| ○誌名 (必須): | □ | Scientific Reports ([Nature Publishing Group])
(eISSN: 2045-2322)
| ○ISSN (任意): | □ | 2045-2322
ISSN: 2045-2322
(eISSN: 2045-2322) Title: Scientific reportsTitle(ISO): Sci RepPublisher: Nature Portfolio (NLM Catalog)
(Scopus)
(CrossRef)
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| ○巻 (必須): | □ | 6
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| ○号 (必須): |
| ○頁 (必須): | □ | 31481 31481
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| ○都市 (任意): |
| ○年月日 (必須): | □ | 西暦 2016年 8月 17日 (平成 28年 8月 17日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1038/srep31481 (→Scopusで検索)
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| ○PMID (任意): | □ | 27530958 (→Scopusで検索)
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| ○機関リポジトリ : | □ | 110153
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| ○備考 (任意): | 1. | (英) Article.ELocationID: 10.1038/srep31481 (日)
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| 2. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
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