『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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登録内容 (EID=317627)

EID=317627EID:317627, Map:0, LastModified:2018年12月13日(木) 19:32:05, Operator:[大家 隆弘], Avail:TRUE, Censor:0, Owner:[小暮 健太朗], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨):
カテゴリ (推奨):
共著種別 (推奨): 国内共著 (徳島大学内研究者と国内(学外)研究者との共同研究 (国外研究者を含まない)) [継承]
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1. (英) Hasan Mahadi (日) (読)
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[継承]
2.田良島 典子 ([徳島大学.大学院医歯薬学研究部.薬学域.薬科学部門.統合医薬創製科学系.生物有機化学])
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[継承]
3. (英) Fujikawa Koki (日) (読)
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[継承]
4. (英) Ohgita Takashi (日) (読)
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[継承]
5. (英) Hama Susumu (日) (読)
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[継承]
6.田中 保 ([徳島大学.大学院社会産業理工学研究部.生物資源産業学域.食料科学系.食料科学分野])
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学籍番号 (推奨):
[継承]
7.斎藤 博幸
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学籍番号 (推奨):
[継承]
8.南川 典昭 ([徳島大学.大学院医歯薬学研究部.薬学域.薬科学部門.統合医薬創製科学系.生物有機化学])
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学籍番号 (推奨):
[継承]
9.小暮 健太朗 ([徳島大学.大学院医歯薬学研究部.薬学域.薬科学部門.創薬科学系.衛生薬学])
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学籍番号 (推奨):
[継承]
題名 (必須): (英) The novel functional nucleic acid iRed effectively regulates target genes following cytoplasmic delivery by faint electric treatment  (日)    [継承]
副題 (任意):
要約 (任意): (英) An intelligent shRNA expression device (iRed) contains the minimum essential components needed for shRNA production in cells, and could be a novel tool to regulate target genes. However, general delivery carriers consisting of cationic polymers/lipids could impede function of a newly generated shRNA via electrostatic interaction in the cytoplasm. Recently, we found that faint electric treatment (fET) of cells enhanced delivery of siRNA and functional nucleic acids into the cytoplasm in the absence of delivery carriers. Here, we examined fET of cells stably expressing luciferase in the presence of iRed encoding anti-luciferase shRNA. Transfection of lipofectamine 2000 (LFN)/iRed lipoplexes showed an RNAi effect, but fET-mediated iRed transfection did not, likely because of the endosomal localization of iRed after delivery. However, fET in the presence of lysosomotropic agent chloroquine significantly improved the RNAi effect of iRed/fET to levels that were higher than those for the LFN/iRed lipoplexes. Furthermore, the amount of lipid droplets in adipocytes significantly decreased following fET with iRed against resistin in the presence of chloroquine. Thus, iRed could be a useful tool to regulate target genes following fET-mediated cytoplasmic delivery with endosomal escape devices.  (日)    [継承]
キーワード (推奨):
発行所 (推奨):
誌名 (必須): Science and Technology of Advanced Materials (IOP Publishing)
(pISSN: 1468-6996, eISSN: 1878-5514)

ISSN (任意): 1468-6996
ISSN: 1468-6996 (pISSN: 1468-6996, eISSN: 1878-5514)
Title: Science and technology of advanced materials
Title(ISO): Sci Technol Adv Mater
Publisher: Taylor & Francis
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 17 [継承]
(必須): 17 [継承]
(必須): 554 562 [継承]
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年月日 (必須): 西暦 2016年 8月 4日 (平成 28年 8月 4日) [継承]
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DOI (任意): 10.1080/14686996.2016.1221726    (→Scopusで検索) [継承]
PMID (任意): 27877903    (→Scopusで検索) [継承]
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Scopus (任意): 2-s2.0-85019070861 [継承]
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備考 (任意): 1.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
2.(英) KeywordList.Keyword: 211 Scaffold / Tissue engineering / Drug delivery  (日)    [継承]
3.(英) KeywordList.Keyword: 30 Bio-inspired and biomedical materials  (日)    [継承]
4.(英) KeywordList.Keyword: 4'-thioDNA  (日)    [継承]
5.(英) KeywordList.Keyword: 600 Others: Nucleic acids  (日)    [継承]
6.(英) KeywordList.Keyword: RNAi effect  (日)    [継承]
7.(英) KeywordList.Keyword: cytoplasmic delivery  (日)    [継承]
8.(英) KeywordList.Keyword: faint electric treatment  (日)    [継承]
9.(英) KeywordList.Keyword: iRed  (日)    [継承]

標準的な表示

和文冊子 ● Mahadi Hasan, Noriko Saito-Tarashima, Koki Fujikawa, Takashi Ohgita, Susumu Hama, Tamotsu Tanaka, Hiroyuki Saito, Noriaki Minakawa and Kentaro Kogure : The novel functional nucleic acid iRed effectively regulates target genes following cytoplasmic delivery by faint electric treatment, Science and Technology of Advanced Materials, Vol.17, No.17, 554-562, 2016.
欧文冊子 ● Mahadi Hasan, Noriko Saito-Tarashima, Koki Fujikawa, Takashi Ohgita, Susumu Hama, Tamotsu Tanaka, Hiroyuki Saito, Noriaki Minakawa and Kentaro Kogure : The novel functional nucleic acid iRed effectively regulates target genes following cytoplasmic delivery by faint electric treatment, Science and Technology of Advanced Materials, Vol.17, No.17, 554-562, 2016.

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