| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○カテゴリ (推奨): |
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| ○組織 (推奨): |
| ○著者 (必須): | 1. | (英) Itakura Shoko (日) (読)
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| 2. | (英) Hama Susumu (日) (読)
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| 3. | (英) Matsui Ryo (日) (読)
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| 4. | 小暮 健太朗 ([徳島大学.大学院医歯薬学研究部.薬学域.薬科学部門.創薬科学系.衛生薬学])
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| ○題名 (必須): | □ | (英) Effective cytoplasmic release of siRNA from liposomal carriers by controlling the electrostatic interaction of siRNA with a charge-invertible peptide, in response to cytoplasmic pH (日)
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| ○要約 (任意): | □ | (英) Condensing siRNA with cationic polymers is a major strategy used in the development of siRNA carriers that can avoid degradation by nucleases and achieve effective delivery of siRNA into the cytoplasm. However, ineffective release of siRNA from such condensed forms into the cytoplasm is a limiting step for induction of RNAi effects, and can be attributed to tight condensation of siRNA with the cationic polymers, due to potent electrostatic interactions. Here, we report that siRNA condensed with a slightly acidic pH-sensitive peptide (SAPSP), whose total charge is inverted from positive to negative in response to cytoplasmic pH, is effectively released via electrostatic repulsion of siRNA with negatively charged SAPSP at cytoplasmic pH (7.4). The condensed complex of siRNA and positively-charged SAPSP at acidic pH (siRNA/SAPSP) was found to result in almost complete release of siRNA upon charge inversion of SAPSP at pH 7.4, with the resultant negatively-charged SAPSP having no undesirable interactions with endogenous mRNA. Moreover, liposomes encapsulating siRNA/SAPSP demonstrated knockdown efficiencies comparable to those of commercially available siRNA carriers. Taken together, SAPSP may be very useful as a siRNA condenser, as it facilitates effective cytoplasmic release of siRNA, and subsequent induction of specific RNAi effects. (日)
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| ○誌名 (必須): | □ | Nanoscale (Royal Society of Chemistry (Great Britain)/Guo jia na mi ke xue zhong xin (China))
(pISSN: 2040-3364, eISSN: 2040-3372)
| ○ISSN (任意): | □ | 2040-3372
ISSN: 2040-3364
(pISSN: 2040-3364, eISSN: 2040-3372) Title: NanoscaleTitle(ISO): NanoscalePublisher: Royal Society of Chemistry (NLM Catalog)
(Scopus)
(CrossRef)
(Scopus information is found. [need login]) | [継承] |
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| ○巻 (必須): | □ | 8
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| ○号 (必須): | □ | 20
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| ○頁 (必須): | □ | 10649 10658
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| ○年月日 (必須): | □ | 西暦 2016年 5月 19日 (平成 28年 5月 19日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1039/c5nr08365f (→Scopusで検索)
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| ○PMID (任意): | □ | 27145993 (→Scopusで検索)
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| ○CRID (任意): |
| ○Scopus (任意): | 1. | 2-s2.0-84971232259
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| ○備考 (任意): | 1. | (英) Article.ELocationID: 10.1039/c5nr08365f (日)
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| 2. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
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