| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○組織 (推奨): | 1. | 徳島大学.大学院ヘルスバイオサイエンス研究部.発達予防医歯学部門.病態予防医学講座.生理機能学 (2004年4月1日〜2015年3月31日)
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| 2. | 徳島大学.医学部.医学科.病態予防医学講座.細胞生物学分野
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| ○著者 (必須): | 1. | (英) Hayashi Mikio (日) 林 美樹夫 (読) はやし みきお
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| 2. | 稲垣 明浩 ([徳島大学.大学院医歯薬学研究部.医学域.特定研究部門.教育支援系.医学教育支援])
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| 3. | (英) Ivana Novak (日) (読)
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| 4. | (英) Matsuda Hiroko (日) 松田 博子 (読) まつだ ひろこ
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| ○題名 (必須): | □ | (英) The adenosine A2B receptor is involved in anion secretion in human pancreatic duct Capan-1 epithelial cells. (日)
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| ○要約 (任意): | □ | (英) Adenosine modulates a wide variety of biological processes via adenosine receptors. In the exocrine pancreas, adenosine regulates transepithelial anion secretion in duct cells and is considered to play a role in acini-to-duct signaling. To identify the functional adenosine receptors and Cl(-) channels important for anion secretion, we herein performed experiments on Capan-1, a human pancreatic duct cell line, using open-circuit Ussing chamber and gramicidin-perforated patch-clamp techniques. The luminal addition of adenosine increased the negative transepithelial potential difference (V te) in Capan-1 monolayers with a half-maximal effective concentration value of approximately 10 M, which corresponded to the value obtained on whole-cell Cl(-) currents in Capan-1 single cells. The effects of adenosine on V te, an equivalent short-circuit current (I sc), and whole-cell Cl(-) currents were inhibited by CFTRinh-172, a cystic fibrosis transmembrane conductance regulator (CFTR) Cl(-) channel inhibitor. The adenosine A2B receptor agonist, BAY 60-6583, increased I sc and whole-cell Cl(-) currents through CFTR Cl(-) channels, whereas the A2A receptor agonist, CGS 21680, had negligible effects. The A2B receptor antagonist, PSB 603, inhibited the response of I sc to adenosine. Immunohistochemical analysis showed that the A2A and A2B receptors colocalized with Ezrin in the luminal membranes of Capan-1 monolayers and in rat pancreatic ducts. Adenosine elicited the whole-cell Cl(-) currents in guinea pig duct cells. These results demonstrate that luminal adenosine regulates anion secretion by activating CFTR Cl(-) channels via adenosine A2B receptors on the luminal membranes of Capan-1 cells. The present study endorses that purinergic signaling is important in the regulation of pancreatic secretion. (日)
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| ○キーワード (推奨): | 1. | アデノシン受容体 (adenosine receptor)
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| 2. | (英) CFTR (日) (読)
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| 3. | (英) Cl channel (日) クロライドチャネル (読) くろらいどちゃねる
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| 4. | (英) Duct (日) 分泌腺導管 (読) ぶんぴせんどうかん
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| 5. | (英) Pancreas (日) 膵臓 (読) すいぞう
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| ○発行所 (推奨): | □ | Springer-Verlag
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| ○誌名 (必須): | □ | Pflügers Archiv : European Journal of Physiology ([Springer-Verlag])
(pISSN: 0031-6768, eISSN: 1432-2013)
| ○ISSN (任意): | □ | 1432-2013
ISSN: 0031-6768
(pISSN: 0031-6768, eISSN: 1432-2013) Title: Pflugers Archiv : European journal of physiologyTitle(ISO): Pflugers ArchSupplier: Springer Online Journal ArchivePublisher: Springer (NLM Catalog)
(Scopus)
(CrossRef)
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| ○巻 (必須): | □ | 468
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| ○号 (必須): | □ | 7
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| ○頁 (必須): | □ | 1171 1181
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| ○年月日 (必須): | □ | 西暦 2016年 7月 1日 (平成 28年 7月 1日)
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| ○DOI (任意): | □ | 10.1007/s00424-016-1806-9 (→Scopusで検索)
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| ○PMID (任意): | □ | 26965147 (→Scopusで検索)
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| ○備考 (任意): | 1. | (英) Article.Affiliation: Medical Research Project, Institute of Biomedical Sciences, Tokushima University Graduate School, 3-18-15 Kuramoto-cho, Tokushima, 770-8503, Japan. (日)
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| 2. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
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| 3. | (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't (日)
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