| ○種別 (必須): | □ | 学術論文 (審査論文)
| [継承] |
| ○言語 (必須): | □ | 英語
| [継承] |
| ○招待 (推奨): |
| ○審査 (推奨): | □ | Peer Review
| [継承] |
| ○カテゴリ (推奨): | □ | 研究
| [継承] |
| ○共著種別 (推奨): | □ | 学内共著 (徳島大学内研究者との共同研究 (学外研究者を含まない))
| [継承] |
| ○学究種別 (推奨): | □ | 博士後期課程学生による研究報告
| [継承] |
| ○組織 (推奨): | 1. | 徳島大学
| [継承] |
| ○著者 (必須): | 1. | 長谷 栄治 ([徳島大学.ポストLEDフォトニクス研究所])
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): | □ | ****
| [ユーザ] |
| [継承] |
| 2. | (英) Matsubara Oki (日) 松原 央樹 (読) まつばら おうき
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): | □ | ****
| [ユーザ] |
| [継承] |
| 3. | 南川 丈夫
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| 4. | 佐藤 克也 ([徳島大学.大学院社会産業理工学研究部.理工学域.機械科学系.知能機械学分野]/[徳島大学.理工学部.理工学科.機械科学コース.知能機械学講座])
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| 5. | 安井 武史 ([徳島大学.ポストLEDフォトニクス研究所]/[徳島大学.理工学部.理工学科.機械科学コース.生産工学講座])
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| ○題名 (必須): | □ | (英) In situ time-series monitoring of collagen fibers produced by standing-cultured osteoblasts using a second-harmonic-generation microscope. (日)
| [継承] |
| ○副題 (任意): |
| ○要約 (任意): | □ | (英) In bone tissue engineering and regeneration, there is a considerable need for an unstained method of monitoring collagen fibers produced by osteoblasts. This is because collagen fibers play an important role as a bone matrix and continuous monitoring of their temporal dynamics is important in clarifying the organization process toward forming bone tissue. In the work described here, using a second-harmonic-generation (SHG) microscope, we performed in situ time-series monitoring of collagen fibers produced by cultured osteoblasts without the need for staining. Use of the 19 fs near-infrared pulsed light enables us to visualize the temporal dynamics in a thin layer of collagen fibers produced by a single layer of osteoblasts in high-contrast SHG images. While the collagen fibers were produced and stored inside the osteoblasts at an early stage of culturing, the network structure of collagen fibers was formed and locally condensed at a late stage. Furthermore, we extracted a quantitative parameter of collagen maturity degree in the cultured sample by use of image analysis based on a two-dimensional Fourier transform of the SHG image. The proposed method will be useful for in situ quality and quantity control of collagen fibers in bone tissue engineering and regeneration. (日)
| [継承] |
| ○キーワード (推奨): |
| ○発行所 (推奨): | □ | Optical Society of America
| [継承] |
| ○誌名 (必須): | □ | Applied Optics ([Optical Society of America])
(pISSN: 1559-128X, eISSN: 1539-4522)
| ○ISSN (任意): | □ | 1539-4522
ISSN: 1559-128X
(pISSN: 1559-128X, eISSN: 1539-4522) Title: Applied opticsTitle(ISO): Appl OptPublisher: Optica Publishing Group (formerly OSA) (NLM Catalog)
(Scopus)
(CrossRef)
(CrossRef)
(Scopus information is found. [need login]) | [継承] |
| [継承] |
| ○巻 (必須): | □ | 55
| [継承] |
| ○号 (必須): | □ | 12
| [継承] |
| ○頁 (必須): | □ | 3261 3267
| [継承] |
| ○都市 (任意): |
| ○年月日 (必須): | □ | 西暦 2016年 4月 20日 (平成 28年 4月 20日)
| [継承] |
| ○URL (任意): |
| ○DOI (任意): | □ | 10.1364/AO.55.003261 (→Scopusで検索)
| [継承] |
| ○PMID (任意): | □ | 27140096 (→Scopusで検索)
| [継承] |
| ○CRID (任意): |
| ○WOS : | □ | 000374388600052
| [継承] |
| ○Scopus (任意): | 1. | 2-s2.0-84964507940
| [継承] |
| ○researchmap (任意): |
| ○評価値 (任意): |
| ○被引用数 (任意): |
| ○指導教員 (推奨): |
| ○備考 (任意): | 1. | (英) PublicationType: Journal Article (日)
| [継承] |