| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○招待 (推奨): |
| ○審査 (推奨): |
| ○カテゴリ (推奨): | □ | 研究
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| ○共著種別 (推奨): | □ | 国内共著 (徳島大学内研究者と国内(学外)研究者との共同研究 (国外研究者を含まない))
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| ○学究種別 (推奨): |
| ○組織 (推奨): |
| ○著者 (必須): | 1. | 刑部 祐里子
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| 2. | 渡辺 崇人 ([徳島大学.バイオイノベーション研究所])
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| 3. | (英) Sugano SS (日) (読)
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| 4. | (英) Ueta R (日) (読)
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| 5. | (英) Ishihara R (日) (読)
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| 6. | (英) Shinozaki K (日) (読)
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| 7. | 刑部 敬史 ([徳島大学.大学院社会産業理工学研究部.生物資源産業学域.生物生産系.生物資源生産科学分野]/[徳島大学.生物資源産業学部.生物資源産業学科.生物生産システムコース])
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| ○題名 (必須): | □ | (英) Optimization of CRISPR/Cas9 genome editing to modify abiotic stress responses in plants. (日)
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| ○要約 (任意): | □ | (英) Genome editing using the CRISPR/Cas9 system can be used to modify plant genomes, however, improvements in specificity and applicability are still needed in order for the editing technique to be useful in various plant species. Here, using genome editing mediated by a truncated gRNA (tru-gRNA)/Cas9 combination, we generated new alleles for OST2, a proton pump in Arabidopsis, with no off-target effects. By following expression of Cas9 and the tru-gRNAs, newly generated mutations in CRIPSR/Cas9 transgenic plants were detected with high average mutation rates of up to 32.8% and no off-target effects using constitutive promoter. Reducing nuclear localization signals in Cas9 decreased the mutation rate. In contrast, tru-gRNA Cas9 cassettes driven by meristematic- and reproductive-tissue-specific promoters increased the heritable mutation rate in Arabidopsis, showing that high expression in the germ line can produce bi-allelic mutations. Finally, the new mutant alleles obtained for OST2 exhibited altered stomatal closing in response to environmental conditions. These results suggest further applications in molecular breeding to improve plant function using optimized plant CRISPR/Cas9 systems. (日)
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| ○キーワード (推奨): |
| ○発行所 (推奨): |
| ○誌名 (必須): | □ | Scientific Reports ([Nature Publishing Group])
(eISSN: 2045-2322)
| ○ISSN (任意): | □ | 2045-2322
ISSN: 2045-2322
(eISSN: 2045-2322) Title: Scientific reportsTitle(ISO): Sci RepPublisher: Nature Portfolio (NLM Catalog)
(Scopus)
(CrossRef)
(Scopus information is found. [need login]) | [継承] |
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| ○巻 (必須): | □ | 6
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| ○号 (必須): | □ |
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| ○頁 (必須): | □ | 26685 26685
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| ○年月日 (必須): | □ | 西暦 2016年 5月 26日 (平成 28年 5月 26日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1038/srep26685 (→Scopusで検索)
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| ○PMID (任意): | □ | 27226176 (→Scopusで検索)
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| ○WOS : | □ | 000376613800001
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| ○Scopus (任意): | 1. | 2-s2.0-84971280492
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| ○機関リポジトリ : | □ | 110151
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| ○備考 (任意): | 1. | (英) Article.ELocationID: 10.1038/srep26685 (日)
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| 2. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
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| 3. | (英) OtherID: PMC4880914 (日)
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