| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○カテゴリ (推奨): |
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| ○著者 (必須): | 1. | (英) Yasuhiro Morita (日) (読)
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| 2. | (英) Masayasu Taniguchi (日) (読)
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| 3. | 谷原 史倫 ([徳島大学.バイオイノベーション研究所])
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| 4. | (英) Aya Ito (日) (読)
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| 5. | (英) Zhao Namula (日) (読)
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| 6. | (英) Lanh Thi Kim DO (日) DO THI KIM LANH (読) ど てぃ きむ らん
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| ○学籍番号 (推奨): | □ | ****
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| 7. | (英) Mitsuhiro Takagi (日) (読)
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| 8. | 竹本 龍也 ([徳島大学.先端酵素学研究所.基幹研究部門])
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| 9. | 音井 威重 ([徳島大学.バイオイノベーション研究所])
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| ○題名 (必須): | □ | (英) The optimal period of Ca-EDTA treatment for parthenogenetic activation of porcine oocytes during maturation culture (日)
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| ○要約 (任意): | □ | (英) The changes triggered by sperm-induced activation of oocytes, which are required for normal oocyte development, can be mediated by other agents, thereby inducing the parthenogenesis. In this study, we exposed porcine oocytes to 1 mM Ca-EDTA, a metal-ion chelator, at various intervals during 48 hr of in vitro maturation to determine the optimum period of Ca-EDTA treatment for parthenogenetic activation. When the oocytes were cultured with or without Ca-EDTA from 36 hr (post-12), 24 hr (post-24), 12 hr (post-36) and 0 hr (post-48) after the start of maturation culture, the blastocyst formation rates were significantly higher (P<0.05) in the post-24, post-36 and post-48 groups (3.3%, 4.0%, and 2.6%, respectively) than those in the control group without treatment (0%). Furthermore, when the oocytes were cultured with Ca-EDTA for 0 hr (control), 12 hr (pre-12), 24 hr (pre-24), 36 hr (pre-36) and 48 hr (pre-48) from the start of maturation culture, the oocytes formed blastocysts only in the pre-36 and pre-48 groups (0.4% or 0.8%, respectively). Pronuclei (<66.7%) were observed only when the periods of Ca-EDTA treatment were more than 12 hr during maturation culture. In the control group, no pronuclei were detected. Our findings demonstrate that porcine immature oocytes can be parthenogenetically activated by Ca-EDTA treatment for at least 24 hr to 36 hr during maturation culture, leading to pronucleus formation followed by the formation of blastocysts. (日)
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| ○キーワード (推奨): |
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| ○誌名 (必須): | □ | The Journal of Veterinary Medical Science ([社団法人 日本獣医学会])
(pISSN: 0916-7250, eISSN: 1347-7439)
| ○ISSN (任意): | □ | 1347-7439
ISSN: 0916-7250
(pISSN: 0916-7250, eISSN: 1347-7439) Title: The Journal of veterinary medical scienceTitle(ISO): J Vet Med SciSupplier: 社団法人日本獣医学会Publisher: Japanese Society of Veterinary Science (NLM Catalog)
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| ○巻 (必須): | □ |
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| ○頁 (必須): | □ |
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| ○年月日 (必須): | □ | 西暦 2016年 3月 4日 (平成 28年 3月 4日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1292/jvms.15-0658 (→Scopusで検索)
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| ○PMID (任意): | □ | 26947170 (→Scopusで検索)
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| ○備考 (任意): | 1. | (英) Article.PublicationTypeList.PublicationType: JOURNAL ARTICLE (日)
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