| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○カテゴリ (推奨): |
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| ○学究種別 (推奨): |
| ○組織 (推奨): |
| ○著者 (必須): | 1. | (英) Hashimoto Masakazu (日) (読)
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| 2. | 竹本 龍也 ([徳島大学.先端酵素学研究所.基幹研究部門])
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| ○貢献度 (任意): |
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| ○題名 (必須): | □ | (英) Electroporation enables the efficient mRNA delivery into the mouse zygotes and facilitates CRISPR/Cas9-based genome editing (日)
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| ○副題 (任意): |
| ○要約 (任意): | □ | (英) Recent use of the CRISPR/Cas9 system has dramatically reduced the time required to produce mutant mice, but the involvement of a time-consuming microinjection step still hampers its application for high-throughput genetic analysis. Here we developed a simple, highly efficient, and large-scale genome editing method, in which the RNAs for the CRISPR/Cas9 system are electroporated into zygotes rather than microinjected. We used this method to perform single-stranded oligodeoxynucleotide (ssODN)-mediated knock-in in mouse embryos. This method facilitates large-scale genetic analysis in the mouse. (日)
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| ○キーワード (推奨): | 1. | (英) Animals (日) (読)
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| 2. | (英) CRISPR-Cas Systems (日) (読)
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| 3. | (英) Electroporation (日) (読)
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| 4. | (英) Female (日) (読)
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| 5. | (英) Gene Knock-In Techniques (日) (読)
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| 6. | (英) Genome (日) (読)
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| 7. | (英) Mice (日) (読)
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| 8. | (英) Mice, Inbred ICR (日) (読)
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| 9. | (英) RNA, Messenger (日) (読)
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| 10. | (英) Zygote (日) (読)
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| ○発行所 (推奨): |
| ○誌名 (必須): | □ | Scientific Reports ([Nature Publishing Group])
(eISSN: 2045-2322)
| ○ISSN (任意): | □ | 2045-2322
ISSN: 2045-2322
(eISSN: 2045-2322) Title: Scientific reportsTitle(ISO): Sci RepPublisher: Nature Portfolio (NLM Catalog)
(Scopus)
(CrossRef)
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| ○巻 (必須): | □ | 5
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| ○号 (必須): | □ |
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| ○頁 (必須): | □ | 11315 11315
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| ○都市 (任意): |
| ○年月日 (必須): | □ | 西暦 2015年 6月 11日 (平成 27年 6月 11日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1038/srep11315 (→Scopusで検索)
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| ○PMID (任意): | □ | 26066060 (→Scopusで検索)
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| ○備考 (任意): | 1. | (英) Article.ELocationID: 10.1038/srep11315 (日)
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| 2. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
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| 3. | (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't (日)
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| 4. | (英) OtherID: PMC4463957 (日)
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