『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=311611EID:311611, Map:0, LastModified:2016年4月26日(火) 18:43:21, Operator:[大家 隆弘], Avail:TRUE, Censor:0, Owner:[鬼塚 正義], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨):
カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1. (英) Matsuyama Rima (日) (読)
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[継承]
2. (英) Tsutsui Tomomi (日) (読)
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学籍番号 (推奨):
[継承]
3. (英) Lee Kyoung Ho (日) (読)
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[継承]
4.鬼塚 正義 ([徳島大学.大学院社会産業理工学研究部.生物資源産業学域.応用生命系.生体分子機能学分野]/[徳島大学.生物資源産業学部.生物資源産業学科.応用生命講座])
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[継承]
5.大政 健史
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[継承]
題名 (必須): (英) Improved gene amplification by cell-cycle engineering combined with the Cre-loxP system in Chinese hamster ovary cells.  (日)    [継承]
副題 (任意):
要約 (任意): (英)   (日) The dihydrofolate reductase gene amplification system is widely used in Chinese hamster ovary (CHO) cells for the industrial production of therapeutic proteins. To enhance the efficiency of conventional gene amplification systems, we previously presented a novel method using cell-cycle checkpoint engineering. Here, we constructed high-producing and stable cells by the conditional expression of mutant cell division cycle 25 homolog B (CDC25B) using the Cre-loxP system. A bispecific antibody-producing CHO DG44-derived cell line was transfected with floxed mutant CDC25B. After inducing gene amplification in the presence of 250 nM methotrexate, mutant CDC25B sequence was removed by Cre recombinase protein expression. Overexpression of the floxed mutant CDC25B significantly enhanced the efficiency of transgene amplification and productivity. Moreover, the specific production rate of the isolated clone CHO Cre-1 and Cre-2 were approximately 11-fold and 15-fold higher than that of mock-transfected clone CHO Mock-S. Chromosomal aneuploidy was increased by mutant CDC25B overexpression, but Cre-1 and Cre-2 did not show any changes in chromosome number during long-term cultivation, as is the case with CHO Mock-S. Our results suggest that high-producing and stable cells can be constructed by conditionally controlling a cell-cycle checkpoint integrated in conventional gene amplification systems.   [継承]
キーワード (推奨):
発行所 (推奨):
誌名 (必須): Journal of Bioscience and Bioengineering ([日本生物工学会])
(pISSN: 1389-1723, eISSN: 1347-4421)

ISSN (任意): 1347-4421
ISSN: 1389-1723 (pISSN: 1389-1723, eISSN: 1347-4421)
Title: Journal of bioscience and bioengineering
Title(ISO): J Biosci Bioeng
Supplier: 公益社団法人日本生物工学会
Publisher: Elsevier BV
 (NLM Catalog  (Webcat Plus  (医中誌Web  (J-STAGE  (Scopus  (CrossRef (Scopus information is found. [need login])
[継承]
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(必須): 120 [継承]
(必須): 6 [継承]
(必須): 701 708 [継承]
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年月日 (必須): 西暦 2015年 6月 21日 (平成 27年 6月 21日) [継承]
URL (任意):
DOI (任意): 10.1016/j.jbiosc.2015.04.009    (→Scopusで検索) [継承]
PMID (任意): 26108159    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) PublicationType: Journal Article  (日)    [継承]
2.(英) PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Rima Matsuyama, Tomomi Tsutsui, Ho Kyoung Lee, Masayoshi Onitsuka and Takeshi Omasa : Improved gene amplification by cell-cycle engineering combined with the Cre-loxP system in Chinese hamster ovary cells., Journal of Bioscience and Bioengineering, Vol.120, No.6, 701-708, 2015.
欧文冊子 ● Rima Matsuyama, Tomomi Tsutsui, Ho Kyoung Lee, Masayoshi Onitsuka and Takeshi Omasa : Improved gene amplification by cell-cycle engineering combined with the Cre-loxP system in Chinese hamster ovary cells., Journal of Bioscience and Bioengineering, Vol.120, No.6, 701-708, 2015.

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