| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○招待 (推奨): |
| ○審査 (推奨): |
| ○カテゴリ (推奨): |
| ○共著種別 (推奨): | □ | 国内共著 (徳島大学内研究者と国内(学外)研究者との共同研究 (国外研究者を含まない))
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| ○学究種別 (推奨): |
| ○組織 (推奨): |
| ○著者 (必須): | 1. | (英) Hasan Mahadi (日) (読)
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| ○学籍番号 (推奨): |
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| 2. | (英) Nishimoto Akinori (日) (読)
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| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
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| 3. | (英) Ohgita Takashi (日) (読)
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| 4. | (英) Hama Susumu (日) (読)
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| ○学籍番号 (推奨): |
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| 5. | (英) Kashida Hiromu (日) (読)
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| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
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| 6. | (英) Asanuma Hiroyuki (日) (読)
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| ○学籍番号 (推奨): |
| [継承] |
| 7. | 小暮 健太朗 ([徳島大学.大学院医歯薬学研究部.薬学域.薬科学部門.創薬科学系.衛生薬学])
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| ○題名 (必須): | □ | (英) Faint electric treatment-induced rapid and efficient delivery of extraneous hydrophilic molecules into the cytoplasm (日)
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| ○要約 (任意): | □ | (英) Effective delivery of extraneous molecules into the cytoplasm of the target cells is important for several drug therapies. Previously, we showed effective in vivo transdermal delivery of naked siRNA into skin cells induced by faint electric treatment (ET) iontophoresis, and significant suppression of target mRNA levels (Kigasawa et al., Int. J. Pharm., 2010). This result indicates that electricity promoted the delivery of siRNA into cytoplasm. In the present study, we analyzed the intracellular delivery of naked anti-luciferase siRNA by faint ET, and found that the luciferase activity of cells expressing luciferase was reduced by in vitro ET like in vivo iontophoresis. Cellular uptake of fluorescent-label siRNA was increased by ET, while low temperature exposure, macropinocytosis inhibitor amiloride and caveolae-mediated endocytosis inhibitor filipin significantly prevented siRNA uptake. These results indicate that the cellular uptake mechanism involved endocytosis. In addition, voltage sensitive fluorescent dye DiBAC4 (3) penetration was increased by ET, and the transient receptor potential channel inhibitor SKF96365 reduced siRNA uptake, suggesting that faint ET reduced membrane potentials by changing intracellular ion levels. Moreover, to analyze cytoplasmic delivery, we used in-stem molecular beacon (ISMB), which fluoresces upon binding to target mRNA in the cytoplasm. Surprisingly, cytoplasmic ISMB fluorescence appeared rapidly and homogeneously after ET, indicating that cytoplasmic delivery is markedly enhanced by ET. In conclusion, we demonstrated for the first time that faint ET can enhance cellular uptake and cytoplasmic delivery of extraneous molecules. (日)
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| ○キーワード (推奨): |
| ○発行所 (推奨): |
| ○誌名 (必須): | □ | Journal of Controlled Release (Controlled Release Society)
(pISSN: 0168-3659, eISSN: 1873-4995)
| ○ISSN (任意): | □ | 1873-4995
ISSN: 0168-3659
(pISSN: 0168-3659, eISSN: 1873-4995) Title: Journal of controlled release : official journal of the Controlled Release SocietyTitle(ISO): J Control ReleasePublisher: Elsevier B.V. (NLM Catalog)
(Scopus)
(CrossRef)
(Scopus information is found. [need login]) | [継承] |
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| ○巻 (必須): | □ | 228
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| ○号 (必須): | □ |
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| ○頁 (必須): | □ | 20 25
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| ○都市 (任意): |
| ○年月日 (必須): | □ | 西暦 2016年 3月 2日 (平成 28年 3月 2日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1016/j.jconrel.2016.02.048 (→Scopusで検索)
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| ○PMID (任意): | □ | 26944781 (→Scopusで検索)
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| ○CRID (任意): |
| ○WOS (任意): | □ | 000373429600003
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| ○Scopus (任意): | □ | 2-s2.0-84961631654
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| ○機関リポジトリ : | □ | 110029
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| ○評価値 (任意): |
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| ○指導教員 (推奨): |
| ○備考 (任意): | 1. | (英) Article.ELocationID: 10.1016/j.jconrel.2016.02.048 (日)
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| 2. | (英) Article.ELocationID: S0168-3659(16)30111-0 (日)
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| 3. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
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| 4. | (英) KeywordList.Keyword: Cellular uptake (日)
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| 5. | (英) KeywordList.Keyword: Cytoplasmic delivery (日)
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| 6. | (英) KeywordList.Keyword: Faint electric treatment (日)
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