『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=308317EID:308317, Map:0, LastModified:2016年2月24日(水) 19:16:31, Operator:[馬場 麻人], Avail:TRUE, Censor:0, Owner:[馬場 麻人], Read:継承, Write:継承, Delete:継承.
種別 (必須):
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨):
カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1. (英) Tseng Yung-Che (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
2. (英) Huang Chang-Jen (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
3. (英) Chang Joshua Chia-Hsi (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
4. (英) Teng Wen-Yuan (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
5.馬場 麻人 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.基礎歯学系.口腔顎顔面形態学])
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
6. (英) Fann Ming-Ji (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
7. (英) Hwang Pung-Pung (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
題名 (必須): (英) Glycogen phosphorylase in glycogen-rich cells is involved in the energy supply for ion regulation in fish gill epithelia.  (日)    [継承]
副題 (任意):
要約 (任意): (英) The molecular and cellular mechanisms behind glycogen metabolism and the energy metabolite translocation between mammal neurons and astrocytes have been well studied. A similar mechanism is proposed for rapid mobilization of local energy stores to support energy-dependent transepithelial ion transport in gills of the Mozambique tilapia (Oreochromis mossambicus). A novel gill glycogen phosphorylase isoform (tGPGG), which catalyzes the initial degradation of glycogen, was identified in branchial epithelial cells of O. mossambicus. Double in situ hybridization and immunocytochemistry demonstrated that tGPGG mRNA and glycogen were colocalized in glycogen-rich cells (GRCs), which surround ionocytes (labeled with a Na(+)-K(+)-ATPase antiserum) in gill epithelia. Concanavalin-A (a marker for the apical membrane) labeling indicated that GRCs and mitochondria-rich cells share the same apical opening. Quantitative real-time PCR analyses showed that tGPGG mRNA expression levels specifically responded to environmental salinity changes. Indeed, the glycogen content, glycogen phosphorylase (GP) protein level and total activity, and the density of tGPGG-expressing cells (i.e., GRCs) in fish acclimated to seawater (SW) were significantly higher than those in freshwater controls. Short-term acclimation to SW caused an evident depletion in the glycogen content of GRCs. Taken altogether, tGPGG expression in GRCs is stimulated by hyperosmotic challenge, and this may catalyze initial glycogen degradation to provide the adjacent ionocytes with energy to carry out iono- and osmoregulatory functions.  (日)    [継承]
キーワード (推奨): 1. (英) Amino Acid Sequence (日) (読) [継承]
2. (英) Animals (日) (読) [継承]
3. (英) Astrocytes (日) (読) [継承]
4. (英) Blotting, Western (日) (読) [継承]
5. (brain) [継承]
6. (英) Cloning, Molecular (日) (読) [継承]
7. (英) DNA, Complementary (日) (読) [継承]
8.エネルギー代謝 (energy metabolism) [継承]
9. (英) Epithelial Cells (日) (読) [継承]
10. (英) Gills (日) (読) [継承]
11. (英) Glycogen (日) (読) [継承]
12. (英) Glycogen Phosphorylase (日) (読) [継承]
13.免疫組織化学 (immunohistochemistry) [継承]
14. (英) In Situ Hybridization (日) (読) [継承]
15. (英) Isoenzymes (日) (読) [継承]
16. (英) Molecular Sequence Data (日) (読) [継承]
17. (英) RNA, Messenger (日) (読) [継承]
18. (英) Reverse Transcriptase Polymerase Chain Reaction (日) (読) [継承]
19. (英) Sodium Chloride (日) (読) [継承]
20. (英) Sodium-Potassium-Exchanging ATPase (日) (読) [継承]
21. (英) Tilapia (日) (読) [継承]
22. (英) Water-Electrolyte Balance (日) (読) [継承]
発行所 (推奨):
誌名 (必須): American Journal of Physiology. Regulatory, Integrative and Comparative Physiology ([アメリカ生理学会])
(pISSN: 0363-6119, eISSN: 1522-1490)

ISSN (任意): 0363-6119
ISSN: 0363-6119 (pISSN: 0363-6119, eISSN: 1522-1490)
Title: American journal of physiology. Regulatory, integrative and comparative physiology
Title(ISO): Am J Physiol Regul Integr Comp Physiol
Publisher: American Physiological Society
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
[継承]
[継承]
(必須): 293 [継承]
(必須): 1 [継承]
(必須): R482 91 [継承]
都市 (任意):
年月日 (必須): 西暦 2007年 3月 15日 (平成 19年 3月 15日) [継承]
URL (任意):
DOI (任意): 10.1152/ajpregu.00681.2006    (→Scopusで検索) [継承]
PMID (任意): 17363679    (→Scopusで検索) [継承]
NAID (任意):
WOS (任意):
Scopus (任意):
評価値 (任意):
被引用数 (任意):
指導教員 (推奨):
備考 (任意): 1.(英) PublicationType: Journal Article  (日)    [継承]
2.(英) PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● (種別) : Yung-Che Tseng, Chang-Jen Huang, Chia-Hsi Joshua Chang, Wen-Yuan Teng, Otto Baba, Ming-Ji Fann and Pung-Pung Hwang : Glycogen phosphorylase in glycogen-rich cells is involved in the energy supply for ion regulation in fish gill epithelia., American Journal of Physiology. Regulatory, Integrative and Comparative Physiology, Vol.293, No.1, R482-91, (発行所), (都市), March 2007.
欧文冊子 ● (種別) : Yung-Che Tseng, Chang-Jen Huang, Chia-Hsi Joshua Chang, Wen-Yuan Teng, Otto Baba, Ming-Ji Fann and Pung-Pung Hwang : Glycogen phosphorylase in glycogen-rich cells is involved in the energy supply for ion regulation in fish gill epithelia., American Journal of Physiology. Regulatory, Integrative and Comparative Physiology, Vol.293, No.1, R482-91, (発行所), (都市), March 2007.

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