| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○組織 (推奨): |
| ○著者 (必須): | 1. | (英) Ochi Arisa (日) (読)
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| 2. | 安倍 知紀
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| 3. | (英) Nakao Reiko (日) (読)
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| 4. | (英) Yamamoto Yoriko (日) (読)
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| 5. | (英) Kitahata Kanako (日) (読)
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| 6. | (英) Takagi Marina (日) (読)
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| 7. | 平坂 勝也
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| 8. | (英) Ohno Ayako (日) (読)
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| 9. | (英) Teshima-Kondo Shigetada (日) (読)
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| 10. | (英) Taesik Gwag (日) (読)
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| 11. | (英) Choi Inho (日) (読)
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| 12. | (英) Kawamura Tomoyuki (日) (読)
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| 13. | 根本 尚夫
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| 14. | 向井 理恵 ([徳島大学.大学院社会産業理工学研究部.生物資源産業学域.食料科学系.食料科学分野])
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| 15. | 寺尾 純二
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| 16. | 二川 健 ([徳島大学.大学院医歯薬学研究部.医学域.栄養科学部門.医科栄養学系.生体栄養学])
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| ○題名 (必須): | □ | (英) N-myristoylated ubiquitin ligase Cbl-b inhibitor prevents on glucocorticoid-induced atrophy in mouse skeletal muscle. (日)
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| ○要約 (任意): | □ | (英) A DGpYMP peptide mimetic of tyrosine(608)-phosphorylated insulin receptor substrate-1 (IRS-1), named Cblin, was previously shown to significantly inhibit Cbl-b-mediated IRS-1 ubiquitination. In the present study, we developed N-myristoylated Cblin and investigated whether it was effective in preventing glucocorticoid-induced muscle atrophy. Using HEK293 cells overexpressing Cbl-b, IRS-1 and ubiquitin, we showed that the 50% inhibitory concentrations of Cbl-b-mediated IRS-1 ubiquitination by N-myristoylated Cblin and Cblin were 30 and 120M, respectively. Regarding the DEX-induced atrophy of C2C12 myotubes, N-myristoylated Cblin was more effective than Cblin for inhibiting the DEX-induced decreases in C2C12 myotube diameter and IRS-1 degradation. The inhibitory efficacy of N-myristoylated Cblin on IRS-1 ubiquitination in C2C12 myotubes was approximately fourfold larger than that of Cblin. Furthermore, N-myristoylation increased the incorporation of Cblin into HEK293 cells approximately 10-folds. Finally, we demonstrated that N-myristoylated Cblin prevented the wet weight loss, IRS-1 degradation, and MAFbx/atrogin-1 and MuRF-1 expression in gastrocnemius muscle of DEX-treated mice approximately fourfold more effectively than Cblin. Taken together, these results suggest that N-myristoylated Cblin prevents DEX-induced skeletal muscle atrophy in vitro and in vivo, and that N-myristoylated Cblin more effectively prevents muscle atrophy than unmodified Cblin. (日)
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| ○キーワード (推奨): |
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| ○誌名 (必須): | □ | Archives of Biochemistry and Biophysics ([Elsevier])
(pISSN: 0003-9861, eISSN: 1096-0384)
| ○ISSN (任意): | □ | 1096-0384
ISSN: 0003-9861
(pISSN: 0003-9861, eISSN: 1096-0384) Title: Archives of biochemistry and biophysicsTitle(ISO): Arch Biochem BiophysPublisher: Academic Press Inc. (NLM Catalog)
(Scopus)
(CrossRef)
(Scopus information is found. [need login]) | [継承] |
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| ○巻 (必須): | □ | 570
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| ○号 (必須): | □ |
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| ○頁 (必須): | □ | 23 31
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| ○都市 (任意): |
| ○年月日 (必須): | □ | 西暦 2015年 2月 14日 (平成 27年 2月 14日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1016/j.abb.2015.02.006 (→Scopusで検索)
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| ○PMID (任意): | □ | 25689493 (→Scopusで検索)
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| ○CRID (任意): |
| ○WOS (任意): | □ | 000352332400004
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| ○Scopus (任意): | □ | 2-s2.0-84924084788
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| ○指導教員 (推奨): |
| ○備考 (任意): | 1. | (英) (日) 「国際会議」として別データ有り EID=295332
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| 2. | (英) Article.ELocationID: 10.1016/j.abb.2015.02.006 (日)
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| 3. | (英) Article.ELocationID: S0003-9861(15)00068-5 (日)
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| 4. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
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| 5. | (英) KeywordList.Keyword: Cbl-b (日)
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| 6. | (英) KeywordList.Keyword: IRS-1 (日)
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| 7. | (英) KeywordList.Keyword: Mice (日)
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| 8. | (英) KeywordList.Keyword: Skeletal muscle atrophy (日)
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| 9. | (英) KeywordList.Keyword: Ubiquitin ligase inhibitor (日)
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