『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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登録内容 (EID=291058)

EID=291058EID:291058, Map:0, LastModified:2015年3月3日(火) 22:00:11, Operator:[山田 久嗣], Avail:TRUE, Censor:0, Owner:[山田 久嗣], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
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審査 (推奨):
カテゴリ (推奨):
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組織 (推奨):
著者 (必須): 1.山田 久嗣 ([徳島大学.大学院社会産業理工学研究部.生物資源産業学域.応用生命系.応用生物資源学分野]/[徳島大学.生物資源産業学部.生物資源産業学科.応用生命講座])
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学籍番号 (推奨):
[継承]
2. (英) Tanabe Kazuhito (日) (読)
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学籍番号 (推奨):
[継承]
3. (英) Nishimoto Sei-ichi (日) (読)
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学籍番号 (推奨):
[継承]
題名 (必須): (英) Fluorometric identification of 5-methylcytosine modification in DNA: combination of photosensitized oxidation and invasive cleavage.  (日)    [継承]
副題 (任意):
要約 (任意): (英) An efficient fluorometric detection system of DNA methylation has been developed by a combination of a photooxidative DNA cleavage reaction with 2-methyl-1,4-naphthoquinone (NQ) chromophore and an invasive cleavage reaction with human Flap endonuclease-1. Enzymatic treatment of a mixture of photochemically fragmented target oligodeoxynucleotides (ODNs) at 5-methylcytosine mC) and hairpin-like probe oligomer possessing a fluorophore (F) and a quencher (D) resulted in a dramatic enhancement of fluorescence. In contrast, fluorescence emission for the ODN containing cytosine but not mC at the target sequence was extremely weak. In addition, by monitoring the fluorescence change, this system allows for the detection of mC in DNA at subfemtomole amounts. This system would provide a highly sensitive protocol for determining the methylation status in DNA by fluorescence emission.  (日)    [継承]
キーワード (推奨): 1. (英) 5-Methylcytosine (日) (読) [継承]
2. (英) Base Sequence (日) (読) [継承]
3. (英) DNA Cleavage (日) (読) [継承]
4. (英) DNA Methylation (日) (読) [継承]
5. (英) Flap Endonucleases (日) (読) [継承]
6. (英) Fluorometry (日) (読) [継承]
7. (英) Humans (日) (読) [継承]
8. (英) Oligonucleotides (日) (読) [継承]
9. (英) Oxidation-Reduction (日) (読) [継承]
10. (英) Photochemistry (日) (読) [継承]
11. (英) Temperature (日) (読) [継承]
12. (英) Vitamin K 3 (日) (読) [継承]
発行所 (推奨):
誌名 (必須): Bioconjugate Chemistry ([アメリカ化学会])
(pISSN: 1043-1802, eISSN: 1520-4812)

ISSN (任意): 1043-1802
ISSN: 1043-1802 (pISSN: 1043-1802, eISSN: 1520-4812)
Title: Bioconjugate chemistry
Title(ISO): Bioconjug Chem
Publisher: American Chemical Society
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 19 [継承]
(必須): 1 [継承]
(必須): 20 23 [継承]
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年月日 (必須): 西暦 2007年 11月 27日 (平成 19年 11月 27日) [継承]
URL (任意):
DOI (任意): 10.1021/bc7003318    (→Scopusで検索) [継承]
PMID (任意): 18038964    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) PublicationType: Journal Article  (日)    [継承]
2.(英) PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Hisatsugu Yamada, Kazuhito Tanabe and Sei-ichi Nishimoto : Fluorometric identification of 5-methylcytosine modification in DNA: combination of photosensitized oxidation and invasive cleavage., Bioconjugate Chemistry, Vol.19, No.1, 20-23, 2007.
欧文冊子 ● Hisatsugu Yamada, Kazuhito Tanabe and Sei-ichi Nishimoto : Fluorometric identification of 5-methylcytosine modification in DNA: combination of photosensitized oxidation and invasive cleavage., Bioconjugate Chemistry, Vol.19, No.1, 20-23, 2007.

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