| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○学究種別 (推奨): |
| ○組織 (推奨): |
| ○著者 (必須): | 1. | (英) Ando Akinori (日) (読)
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| 2. | 櫻谷 英治 ([徳島大学.大学院社会産業理工学研究部.生物資源産業学域.食料科学系.食料科学分野]/[徳島大学.生物資源産業学部.生物資源産業学科.食料科学コース])
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| 3. | (英) Horinaka Kota (日) (読)
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| 4. | (英) Ogawa Jun (日) (読)
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| 5. | (英) Shimizu Sakayu (日) (読)
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| ○題名 (必須): | □ | (英) Transformation of an oleaginous zygomycete Mortierella alpina 1S-4 with the carboxin resistance gene conferred by mutation of the iron-sulfur subunit of succinate dehydrogenase. (日)
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| ○要約 (任意): | □ | (英) The sdhB gene encoding an iron-sulfur (Ip) subunit of succinate dehydrogenase (SDH, EC 1.3.99.1) complex was cloned from Mortierella alpina 1S-4. The deduced amino acid sequence of SdhB from M. alpina 1S-4 showed high similarity to those of SdhB from other organisms. The mutated sdhB (CBXB) gene encodes a modified SdhB with an amino-acid substitution (a highly conserved histidine residue within the third cysteine-rich cluster of SdhB replaced by a leucine residue) and is known to confer carboxin resistance. We succeeded in transforming M. alpina 1S-4 by using the CBXB gene as a selectable marker gene and expressing the heterologous uidA gene encoding beta-glucuronidase of Escherichia coli. Moreover, transformation efficiency was up to 40-50 transformants per 4.0 x 10(8) spores. This carboxin-transformation system, characterized by marginal background growth and mitotic stability in M. alpina 1S-4, is considered to be widely useful for the wild strain, M. alpina 1S-4, and various derivative mutants without laborious preparation of auxotrophic mutants as a host strain. (日)
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| ○キーワード (推奨): | 1. | (英) Amino Acid Sequence (日) (読)
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| 2. | (英) Blotting, Southern (日) (読)
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| 3. | (英) Carboxin (日) (読)
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| 4. | (英) DNA, Fungal (日) (読)
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| 5. | (英) Drug Resistance, Fungal (日) (読)
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| 6. | (英) Fungicides, Industrial (日) (読)
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| 7. | (英) Glucuronidase (日) (読)
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| 8. | (英) Molecular Sequence Data (日) (読)
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| 9. | (英) Mortierella (日) (読)
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| 10. | (英) Mutation (日) (読)
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| 11. | (英) Protein Subunits (日) (読)
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| 12. | (英) Sequence Analysis, DNA (日) (読)
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| 13. | (英) Sequence Homology, Amino Acid (日) (読)
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| 14. | (英) Succinate Dehydrogenase (日) (読)
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| 15. | (英) Transformation, Genetic (日) (読)
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| ○発行所 (推奨): |
| ○誌名 (必須): | □ | Current Genetics ([Springer-Verlag])
(pISSN: 0172-8083, eISSN: 1432-0983)
| ○ISSN (任意): | □ | 1432-0983
ISSN: 0172-8083
(pISSN: 0172-8083, eISSN: 1432-0983) Title: Current geneticsTitle(ISO): Curr GenetSupplier: Springer Online Journal ArchivePublisher: Springer (NLM Catalog)
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| ○巻 (必須): | □ | 55
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| ○号 (必須): | □ | 3
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| ○頁 (必須): | □ | 349 356
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| ○年月日 (必須): | □ | 西暦 2009年 5月 23日 (平成 21年 5月 23日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1007/s00294-009-0250-1 (→Scopusで検索)
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| ○PMID (任意): | □ | 19466416 (→Scopusで検索)
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| ○備考 (任意): | 1. | (英) PublicationType: Journal Article (日)
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| 2. | (英) PublicationType: Research Support, Non-U.S. Gov't (日)
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