『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=280273EID:280273, Map:0, LastModified:2014年9月17日(水) 09:59:11, Operator:[松井 栄里], Avail:TRUE, Censor:0, Owner:[木戸 淳一], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
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審査 (推奨):
カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1.中島 由紀子 ([徳島大学.病院.診療科.歯科.歯周病科(第二保存科)])
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2.稲垣 裕司 ([徳島大学.病院.診療科.歯科.歯周病科(第二保存科)])
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3.廣島 佑香 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.基礎歯学系.口腔微生物学])
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4.木戸 淳一 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.臨床歯学系.歯周歯内治療学])
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学籍番号 (推奨):
[継承]
5.永田 俊彦
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題名 (必須): (英) Advanced glycation end-products enhance calcification in cultured rat dental pulp cells  (日)    [継承]
副題 (任意):
要約 (任意): (英) Amorphous calcification frequently appears in dental pulp tissues of diabetic patients; however, its pathologic process has not been fully elucidated. We previously found that pulp stones and thickened predentin occurred more frequently in diabetic rats. Recent findings demonstrated that accumulation of advanced glycation end-products (AGE) might be involved in vascular calcification complicated with diabetes. The aim of this study was to determine the effect of AGE on calcified nodule formation by rat dental pulp cells in culture. Rat dental pulp cells and gingival fibroblasts were independently cultured with 50 and 100 μg/mL AGE. Alkaline phosphatase activity and calcified nodule formation were measured. Expressions of receptor for AGE, osteopontin (OPN), and osteocalcin (OCN) mRNA were determined by quantitative real-time polymerase chain reaction. Protein levels of OPN and OCN secreted in culture medium were quantified by enzyme-linked immunosorbent assay. AGE (50 and 100 μg/mL) markedly increased both alkaline phosphatase activity and calcified nodule formation in dental pulp cells (P < .01), whereas it did not affect those in gingival fibroblasts. Real-time polymerase chain reaction analysis revealed that AGE increased mRNA expressions of receptor for AGE, OPN, and OCN in dental pulp cells (P < .05). Enzyme-linked immunosorbent assay analysis revealed that the protein levels of OPN and OCN produced by dental pulp cells were higher in AGE-treated than in untreated cells (P < .05). AGE enhanced the calcification potentials of rat dental pulp cells, suggesting that it may stimulate pathologic calcification of diabetic dental pulp tissues.  (日)    [継承]
キーワード (推奨): 1. (英) Alkaline Phosphatase (日) (読) [継承]
2. (英) Animals (日) (読) [継承]
3. (英) Cell Culture Techniques (日) (読) [継承]
4. (英) Cells, Cultured (日) (読) [継承]
5. (英) Dental Pulp (日) (読) [継承]
6. (英) Dental Pulp Calcification (日) (読) [継承]
7. (英) Fibroblasts (日) (読) [継承]
8. (英) Gingiva (日) (読) [継承]
9. (英) Glycosylation End Products, Advanced (日) (読) [継承]
10. (英) Male (日) (読) [継承]
11. (英) Osteocalcin (日) (読) [継承]
12. (英) Osteopontin (日) (読) [継承]
13. (英) Rats (日) (読) [継承]
14. (英) Rats, Wistar (日) (読) [継承]
15. (英) Receptors, Immunologic (日) (読) [継承]
16. (英) Time Factors (日) (読) [継承]
発行所 (推奨):
誌名 (必須): Journal of Endodontics ([The American Association of Endodontics])
(pISSN: 0099-2399, eISSN: 1878-3554)

ISSN (任意): 1878-3554
ISSN: 0099-2399 (pISSN: 0099-2399, eISSN: 1878-3554)
Title: Journal of endodontics
Title(ISO): J Endod
Publisher: Elsevier Inc.
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 873 878 [継承]
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年月日 (必須): 西暦 2013年 1月 16日 (平成 25年 1月 16日) [継承]
URL (任意):
DOI (任意): 10.1016/j.joen.2012.11.027    (→Scopusで検索) [継承]
PMID (任意): 23791254    (→Scopusで検索) [継承]
NAID (任意):
WOS (任意): 000321881100005 [継承]
Scopus (任意): 2-s2.0-84879412695 [継承]
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備考 (任意): 1.(英) Article.ELocationID: 10.1016/j.joen.2012.11.027  (日)    [継承]
2.(英) Article.ELocationID: S0099-2399(12)01085-0  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
4.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Yukiko Nakajima, Yuji Inagaki, Yuka Hiroshima, Jun-ichi Kido and Toshihiko Nagata : Advanced glycation end-products enhance calcification in cultured rat dental pulp cells, Journal of Endodontics, Vol.39, 873-878, 2013.
欧文冊子 ● Yukiko Nakajima, Yuji Inagaki, Yuka Hiroshima, Jun-ichi Kido and Toshihiko Nagata : Advanced glycation end-products enhance calcification in cultured rat dental pulp cells, Journal of Endodontics, Vol.39, 873-878, 2013.

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