『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=278986EID:278986, Map:0, LastModified:2017年11月27日(月) 18:34:04, Operator:[大家 隆弘], Avail:TRUE, Censor:0, Owner:[湯浅 智之], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨): Peer Review [継承]
カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1.湯浅 智之 ([徳島大学.大学院医歯薬学研究部.医学域.連携研究部門(医学域).寄附講座系(医学域).実践地域診療・医科学])
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学籍番号 (推奨):
[継承]
2. (英) Amo Kikuko (日) (読)
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貢献度 (任意):
学籍番号 (推奨):
[継承]
3. (英) Ishikura Shuhei (日) (読)
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学籍番号 (推奨):
[継承]
4. (英) Nagaya Hisao (日) (読)
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学籍番号 (推奨):
[継承]
5.内山 圭司 ([徳島大学.先端酵素学研究所.次世代酵素学研究領域])
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学籍番号 (推奨):
[継承]
6. (英) Hashida Seiichi (日) (読)
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貢献度 (任意):
学籍番号 (推奨):
[継承]
7. (英) Ebina Yousuke (日) (読)
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貢献度 (任意):
学籍番号 (推奨):
[継承]
題名 (必須): (英) Development of in vitro model of insulin receptor cleavage induced by high glucose in HepG2 cells.  (日)    [継承]
副題 (任意):
要約 (任意): (英) Soluble insulin receptor (sIR), the ectodomain of IR, has been detected in human plasma, and its concentration parallels that of blood glucose in patients with diabetes. IR has a pivotal role in glucose homeostasis and diabetes development; therefore, cleavage of IR promoted by hyperglycemia is involved in insulin resistance and glucose toxicity. To elucidate the physiology of sIR, we developed an in vitro model mimicking the changes in sIR levels in plasma from patients with diabetes. Among four human cell lines that expressed IR, spontaneous cleavage of IR occurred only in HepG2 cells. The molecular characteristics of sIR derived from HepG2 cells were similar to those of sIR detected in human plasma. The concentration of sIR in the medium did not differ between basal and high-glucose conditions in the initial 24-h period, but increasing the duration of pre-stimulation (>48 h) led to a significant increase in sIR levels in cells exposed to high glucose. Additionally, glucose-dependent increment of sIR was reversible in this model. These results are consistent with the observation of plasma sIR in patients with diabetes. Using this model, O-linked N-acetylglucosamine modification was determined to be involved in high-glucose-induced IR cleavage. A calcium-dependent protease was shown to cleave IR extracellularly. These findings show that this in vitro model could be useful for determining the molecular mechanism underlying IR cleavage.  (日)    [継承]
キーワード (推奨): 1. (英) soluble insulin receptor (日) (読) [継承]
2. (英) Insulin resistance (日) (読) [継承]
3. (英) shedding (日) (読) [継承]
4. (英) O-GlcNAc (日) (読) [継承]
5. (英) Calcium dependent protease (日) (読) [継承]
6. (英) Diabetes (日) (読) [継承]
7. (英) Hep G2 (日) (読) [継承]
発行所 (推奨):
誌名 (必須): Biochemical and Biophysical Research Communications ([Elsevier])
(pISSN: 0006-291X, eISSN: 1090-2104)

ISSN (任意): 1090-2104
ISSN: 0006-291X (pISSN: 0006-291X, eISSN: 1090-2104)
Title: Biochemical and biophysical research communications
Title(ISO): Biochem. Biophys. Res. Commun.
Publisher: Elsevier Inc.
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 445 [継承]
(必須): 1 [継承]
(必須): 236 243 [継承]
都市 (任意):
年月日 (必須): 西暦 2014年 2月 6日 (平成 26年 2月 6日) [継承]
URL (任意):
DOI (任意): 10.1016/j.bbrc.2014.01.187    (→Scopusで検索) [継承]
PMID (任意): 24508798    (→Scopusで検索) [継承]
NAID (任意):
WOS (任意): 000332749400041 [継承]
Scopus (任意): 2-s2.0-84896697427 [継承]
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備考 (任意): 1.(英) PublicationType: Journal Article  (日)    [継承]

標準的な表示

和文冊子 ● Tomoyuki Yuasa, Kikuko Amo, Shuhei Ishikura, Hisao Nagaya, Keiji Uchiyama, Seiichi Hashida and Yousuke Ebina : Development of in vitro model of insulin receptor cleavage induced by high glucose in HepG2 cells., Biochemical and Biophysical Research Communications, Vol.445, No.1, 236-243, 2014.
欧文冊子 ● Tomoyuki Yuasa, Kikuko Amo, Shuhei Ishikura, Hisao Nagaya, Keiji Uchiyama, Seiichi Hashida and Yousuke Ebina : Development of in vitro model of insulin receptor cleavage induced by high glucose in HepG2 cells., Biochemical and Biophysical Research Communications, Vol.445, No.1, 236-243, 2014.

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